186802-Model hc1

HumaClot Junior
| Service Manual
Cat No. 18680/02

REVISION LIST OF THE MANUAL
Rev. /DATE. REVISION DESCRIPTION
01/2018-01 First edition
SYSTEM VERSION
COPYRIGHT
Copyright 2017, Human Gesellschaft für Biochemica und Diagnostica mbH, Wiesbaden,
Germany. All rights reserved.
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of HUMAN in
writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
SERVICE UND SUPPORT

CONTENTS
TABLE OF CONTENTS
1 SAFETY INSTRUCTIONS 5
1.1 INTRODUCTION 5
1.2 USER WARRANTY 5
1.3 INTENDED USE OF THE INSTRUMENT 5
1.4 GENERAL SAFETY WARNINGS 6
1.5 DISPOSAL MANAGEMENT CONCEPT 6
1.6 BIOHAZARD WARNING 7
1.7 INSTRUMENT DISINFECTION 7
1.8 REPAIRS 7
2 INTRODUCTION 9
2.1 SERVICE MODE 9
2.1.1 Access to the service menu 9
2.1.2 Service functions 10
2.1.3 Quit service mode 23
2.2 USER MODE 24
2.2.1 UTILITIES menu 24
2.2.2 Access to the UTILITIES Menu 24
2.2.3 Functions of the UTILITIES Menu 25
2.2.4 Quit UTILITIES menu 33
2.2.5 PIN-Code forgotten 34
3 ADJUSTMENTS 37
3.1 REPAIR MATRIX 37
3.2 SERVICE CHECK 38
3.3 LCD CONTRAST 39
3.4 SPEED SENSOR DISTANCE 40
3.5 MIXER SPINNING 41
3.6 CHECK THERMAL BLOCK TEMPERATURE 41
3.7 CALIBRATE THERMAL BLOCK TEMPERATURE 43
3.8 SOFTWARE UPDATE 47
3.9 PARAMETER UPDATE 52
3.9.1 Parameter Import 52
3.9.2 Automatic Parameter Import 58
3.9.3 Parameter Export 64
3.9.4 Parameter Error 70
3.10 PARAMETER PROTOCOL 71
3.11 PRINTER CONNECTION 82
3.12 DPU 414 PRINTER SETUP 85
3.13 LOAD CUVETTECARD 89
3.14 PHOTOMETER CALIBRATION 93
3.15 VIRTUAL COMPORT DRIVER 96
4 INSTRUMENT ERRORS 97
5 DISASSEMBLING / ASSEMBLING 117
6 HARDWARE COMPONENTS 123

6.1 PRINTED CIRCUIT BOARDS 123
6.2 MEMBRANE KEYBOARD 125
6.3 HOUSINGS 126
6.4 CABLES 128
6.5 THERMAL BLOCK 133
6.6 SMALL PARTS 134
6.7 BLOCK WIRING DIAGRAM 136
6.8 CONNECTORS PINOUT 137
6.9 PIN ASSIGNMENT 138
7 APPENDIX 143
7.1 SOFTWARE STRUCTURE 143
CONTENTS
Safety Instructions 5
1 SAFETY INSTRUCTIONS
1.1 Introduction
This manual is considered part of the instrument and must be available to the
operator and the maintenance personnel. For accurate installation, use and
maintenance, please read the following instructions carefully.
In order to avoid damage to the instrument or personal injury, carefully read
the ”GENERAL SAFETY WARNINGS”, describing the appropriate operating pro-
cedures. Please contact your HUMAN authorised local Technical Service in the
event of instrument failure or other difficulties with the instrument.
1.2 User Warranty

HUMAN warrants that instruments sold by one of its authorised representa-
tives shall be free of any defect in material or workmanship, provided that this
warranty shall apply only to defects which become apparent within one year
from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item within this
warranty period at no charge, except for transportation expenses to the point
of repair.
This warranty excludes the HUMAN representative from liability to replace any
item considered as expendable in the course of normal usage, e.g.: lamps,valves,
syringes, glassware, fuses, tubing etc.
The HUMAN representative shall be relieved of any liability under this warranty
if the product is not used in accordance with the manufacturer‘s instructions,
altered in any way not specified by HUMAN, not regularly maintained, used with
equipment not approved by HUMAN or used for purposes for which it was not
designed.
1.3 Intended Use of the Instrument

The instrument must be used for its intended purpose (see paragraph 2). It must
be operated in perfect technical conditions, by qualified personnel, in such [IVD]
working conditions and maintained as described in this manual, in the GENERAL
SAFETY WARNINGS. This manual contains instructions for qualified professional
operators.
6
1.4 General Safety Warnings

Use only chemical reagents and accessories specified and supplied by HUMAN
and/or mentioned in this manual. Place the product so that it has proper ven-
tilation.
The instrument should be installed on a flat, stationary working surface, that is
free of vibrations.
Do not operate in area with excessive dust.
Operate at temperature and at a humidity level in accordance with the specifi-
cations listed in this manual.
Do not operate this instrument with covers and panels removed.
Use only the power cord specified for this product, with the grounding
conductor of the power cord connected to earth ground.
Use only the fuse type and rating specified by the manufacturer for this instru-
ment.
The use of fuses with improper ratings may pose electrical and fire hazards.
To avoid fire or shock hazard, observe all ratings and markings on the instru-
ment.
Do not power the instrument in environments that are potentially explosive or
at risk of fire.
Prior to cleaning and/or performing maintenance on the instrument, switch off
the instrument and remove the power cord.
Only cleaning materials described in this manual may be used, as other mate-
rials may damage parts. It is recommended to always wear protective clothing
and eye protection while using this instrument.
All warning symbols that appear in this manual must be carefully observed.
1.5 Disposal Management Concept

The applicable local regulations governing disposal must be observed. It is the
user‘s responsibility to arrange for proper disposal of the individual components.
All parts which may contain potentially infectious materials must be disinfect-
ed by suitable, validated procedures (autoclaving, chemical treatment) prior to
disposal. Applicable local regulations for disposal must be carefully observed.
The instruments and electronic accessories (without batteries, power packs etc.)
must be disposed of according to the applicable local regulations for the dispos-
al of electronic components.
Batteries, power packs and similar power sources must be removed from elec-
tric/electronic parts and disposed of in accordance with applicable local regula-
tions.
HumaClot Junior | Service manual

Safety Instructions 7
1.6 Biohazard Warning

Analytical instruments for in vitro diagnostic application involve the handling of
human samples and controls which should be considered at least potentially in-
fectious. Therefore every part and accessory of the respective instrument which
may have come into contact with such samples must equally be considered as
potentially infectious.
The „BIOHAZARD“ warning label must be affixed to the instrument prior to first
use with biological material!
Figure 1
Biological Hazard Symbol
1.7 Instrument Disinfection

Before performing any servicing on the instrument it is very important to thor-
oughly disinfect all possibly contaminated parts. Before the instrument is re-
moved from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination must be performed by authorised well-trained personnel, and
in observance of all necessary safety precautions.
1.8 Repairs
-- For all the operations described in this manual, wear latex

or nitrile gloves and wash hands thoroughly after comple-
tion of work.
-- Be careful with the sharp edges of the metal sheets after
removal of the covers.
8

Introduction 9
2 INTRODUCTION
2.1 SERVICE MODE

This section describes the possibility to check individual modules and functions
of the HumaClot Junior operated by authorized staff using the service code.
2.1.1 ACCESS TO THE SERVICE MENU

The service mode is part of the installed software. Special number code grants
accesses for the service technician, to access the hidden service menu do the
following steps:
-- Switch on the Analyzer by plugging the power supply, the below message
will be displayed:
-- While the line is scrolling then it is the only possible way to enter the service
menu by entering the service code 22926.
-- If the service code was entered correctly this message below will be dis-
played:
! Note: Each keystroke is confirmed by a beep tone.
It is not possible to correct the service code.
To re-enter the service code the Analyzer should be turned off

then turned on.
By pressing the left and the right cursor keys you can select be-
tween test functions, calibration functions, and parameter func-
tions by confirm it with Enter Key
10
Warning
The Service Code is confidential and may not be accessed by unauthorized
person.
2.1.2 SERVICE FUNCTIONS

The service mode has different test functions, calibration functions, and param-
eter functions, by pressing on Right or Left cursor key you can select any of the
test functions, calibration functions, and parameter function by pressing Enter
key.
Test Functions
1. Photometer calibration
2. Optic-test
3. Channel-test
4. Mixer-test
5. Heating-test
6. Port A-test
7. Port B-test
8. Keyboard-test
9. Display-test
10. Chip-card-test
11. SD-card-test
12. Ext 5V-upply-test
13. Nebenlicht-test (ambient light test)
Calibration functions
1. Calibration temperature
2. Default parameters
Parameter functions
1. Parameter export
2. Parameter import

Introduction 11
Photometer calibration
-- Press Enter key when < photometer calibr. > is displayed.
-- When this message below is displayed remove the cuvette from the meas-
uring channel, close the light protection cap and keep the cap closed while
processing then press Enter key.
-- When this message below is displayed press Enter key to complete the test
or you can exit the function by pressing the ESC key.
For detailed procedure please see chapter 3.14.
Optic-test
-- Press Enter key when <optic-test> is displayed.
-- The below message will be displayed after the optic test is completed (The
below values are an example only).
12
CH1 Measuring channel 1

L1 Light Source 1
55617 Actual value for measuring channel [digits]
41873 Actual value for reference channel [digits]
100, 00% Actual value for transmission [%]
0,000E Actual value for extinction [E]
160 Actual photometer calibration value for light source
-- You can leave this function by pressing the ESC key and the LED will be
switched off.
Channel-test
-- Press Enter key when < channel-test > is displayed.
-- The below message will be displayed after the optic test is completed (The
below values are an example only).
LEDx = 0 Actual target value for LED

G = OFF Actual status for (Gain) amplifier for measuring signal
Ref = 267 Actual value for Reference channel
Meas = 183 Actual value for measuring channel
! Note: The values can be diffe-

rent from Analyzer to Analy-
zer and for each channel.
-- Enter the target value for the LED and confirm it with the Enter key and it
will have the necessary brightness, also the received signal from the photo
sensor for the Reference and Measuring channels will be displayed.
-- You can leave this function press ESC key and the LED will be switched OFF.
The acceptable ranges:
Table 1
Minimum Maximum Unit
LED 0 1000 Digit 0 = off
Meas 0 65535 Digit
Ref 0 65535 Digit

Introduction 13
Mixer-test
-- Press Enter key when < mixer-test > is displayed.
-- The below message will be displayed after the Mixer-test is completed (The
below values are an example only), to leave this function press ESC key and
the motor will keep spinning.
Motor 0  The actual target value for mixer motor speed [rpm]
rpm 0  The present value of mixer motor speed [rpm]
-- Enter the target motor revolution value and confirm with Enter.
-- The motor will turn accordingly.
-- The receiving signal of the hall-sensor on PCB will displayed as the revolu-
tions.
The acceptable ranges:
Minimum Maximum Unit Table 2

Motor 0 0 = off
200 999 Rpm
Table 3
Minimum Maximum Unit Tolerance
Motor 200 999 Rpm + / - 50
Heating-test
With this function you can check the heating and the temperature sensor in the
thermal block.
14
-- Press Enter key when < heating-test > is displayed.
-- The below message will be displayed after the heating test is completed (The
below values are an example only), to leave this function press ESC key, but
before leaving the function put the heating back to 0% and it will switch it
off.
Heating 0%  Actual target value heating power [%]

NTC: 54399  Present value of NTC temperature sensor [digits]
-- Enter the target heating power value [%] and confirm it with Enter.
-- The heater will start to heat up the thermal block according the selected
value.
-- The receiving signal of the NTC temperature sensor on PCB will be displayed
as digits.
-- Increasing values = thermal block is getting colder.
-- Decreasing values = thermal block is getting warmer.
-- When the test starts the heating will be turned off which means it will be 0%.
-- Enter a value between 1 and 100% and the heating will be switched on by
checking the pulse-pause relation.
-- By reading the NTC value you can check whether the temperature sensor
and the heating are working, if this value decreases while the thermal block
temperature rises then the heating is switched on.
Important:
In this mode it is possible to heat the thermal block up to 60°C.
The Acceptable ranges:
Table 4
Minimum Maximum Unit
Heating 0 100 % 0 = off

Introduction 15
Port A-test
With this function the serial port (Port A) can be checked, therefore special tests
plug which can be ordered as „service tool“.
-- Press Enter key when < Port A-test > is displayed
-- The below message will be displayed after the Port A-test is completed (The
below values are an example only), to leave this function press ESC key.
-- The functions TxD => RxD will be checked (loop back).

-- After plugging in the test plug the buttons of the membrane keyboard can
be pressed and will be confirmed by a beeping signal.
-- If the serial port works the key code will be displayed in combination with
second beep.
-- With the Enter key you will start or stop repeat mode which will continuous-
ly transmit the code for the last pressed key to the serial port.
The key codes:
Key Display Key Display Table 5

0 Number 0 Start Channel 1
1 Number 1
2 Number 2
3 Number 3
4 Number 4
5 Number 5
6 Number 6
7 Number 7
8 Number 8
9 Number 9
< Cursor left
> Cursor right
Mode Mode
Enter Enter
ESC ESC Test ends now
16
Port B-test
With this function the USB Port (virtual ComPort) can be checked and the com-
munication between HumaClot Junior and the Computer can be tested, There-
fore an USB Cable, a computer, a virtual ComPort driver and terminal software
are required. If the USB port works a character wise communication between
terminal software and HumaClot Junior is possible.
-- Press Enter key when < Port B-test > is displayed.
-- The below message will be displayed after the Port B-test is completed(The
below values are an example only), to leave this function press ESC key.
-- With the Enter key you will start or stop repeat mode which will continuous-
ly transmit the code for the last pressed key to the serial port.
The key codes:
Table 6
Key Display Key Display
1 Number 1
2 Number 2
3 Number 3
4 Number 4
5 Number 5
6 Number 6
7 Number 7
8 Number 8
9 Number 9
< Cursor left
> Cursor right
Mode Mode
Enter Enter

Introduction 17
Keyboard- test
With this function the keyboard can be tested, the keys of the membrane key-
board can be pressed and will be confirmed by a beeping signal and shown in
the display.
-- Press Enter key when < keyboard-test > is displayed.
-- Press any key when the message below is displayed, to leave this function
press ESC key.
The key codes:
Key Display Key Display Table 7

1 Number 1
2 Number 2
3 Number 3
4 Number 4
5 Number 5
6 Number 6
7 Number 7
8 Number 8
9 Number 9
< Cursor left
> Cursor right
Mode Mode
Enter Enter
18
Display-test
With this function the display can be tested.
-- Press Enter key when < display-test > is displayed:
-- Press any key when the message below is displayed, to leave this function
press ESC key:
-- The test will automatically fill the display screen with single characters.
-- After that a character set will be shown automatically.
ChipCARD-test
! Note: A valid CuvetteCARD

(CuvCARD) or a Chipcard with
application parameters will be
With this function the chip card reading unit on the right side of the instrument
can be tested. Press Enter key when < chipcard-test > is displayed:
erased and devaluated by this

test.
-- When this message below is displayed please insert the chip card, to leave
this function press ESC key:
-- The readers unit will be tested by inserting and removing the chip card.
Table 8
Display shows Explanation / Reason
No card inserted
insert card
No card detected
Test failed
Reading or writing or card detect not possible
test failed
Check for correct direction/insertion of card
Check for correct card type
Card detected
please wait
Access to card in process
Test result OK
test ok
read / write access to card worked
Card detected
may remove card
Card can be removed

Introduction 19
Ext 5V-supply-test
The HumaClot Junior provides (5VDC) voltage for an external Barcode Reader
plugged at (RS232) port, for this test a (RS232-test plug) is required to check the
outgoing signal.
-- Press Enter key when < ext 5V-supply-test > is displayed:
-- When this message below is displayed:
Pressing 1 key switches 5VDC on (indicator LED ON)

Pressing 0 key switches 5VDC off (indicator LED OFF)
-- To leave this function press ESC key.
Nebenlicht test (ambient light test)

The HumaClot Junior photometer is equipped with an ambient light compen-
sation.
-- Press Enter key when < nebenlicht test > is displayed:
-- This message below will be displayed (To leave this function press ESC key):
LED =0  Target value for LED

G =0  Actual status for (Gain) amplifier for measuring signal
B 184  Actual bright value
D 184  Actual dark value
=0  Actual value of measuring channel
20
Example about the previous test:

-- Assume the target value for LED=100.
-- Press Enter key to confirm .
-- The Actual Bright value “B” will rise up  B=347xx
The Actual Dark value “D” should be stable  D=184
-- The measuring value should be the actual bright value minus the actual dark
value.
-- Light into the measuring channel with a lamp (without using an LED or en-
ergy saving lamp).
-- Depending on this amount of light the actual bright value and the actual
dark value will rise up.
-- Light compensation should keep the value of the measuring channel nearly
stable.
-- The measuring value should be the actual bright value minus the actual dark
value. The actual bright values − the actual dark value = 345xx.
Calibration functions
Calibration temperature
This function is intended to calibrate the thermal block temperature to a target
value of 37.4 °C, for detailed calibration process please see chapter 3.7.
-- Press Enter key when < calib. temperature > is displayed:
-- This message below will be displayed (to leave this function press ESC key):
HumaClot Junior actual adjustment temperatures which

Intern 37.4 
will be adjusted to 37.4°C automatically.
NTC 54302  Actual value of NTC temperature sensor.
Input box for externally measured temperature of ther-
Extn 37.4 
mal block.

Introduction 21
Default parameters
This function is intended to load a default parameter setup from the memory of
the Analyzer, but this function will be processed without a 2nd safety query, so
any existing balanced cuvette will be deleted!
-- Press Enter key when < default parameters > is displayed:
-- This message below will be displayed:
-- By pressing > key the displayed message will change as shown below:
-- Press Enter key.
-- When this message below is displayed, that’s mean the factory defaults
parameters have been loaded back to EEPROM:
-- Press ESC key when this below message is displayed:

22
-- Check / calibrate thermal block temperature.

-- Calibrate photometer.
-- Check application parameters.
-- Check / re-enter conversion parameters.
-- Load new cuvette balance.
-- Contact distributor regarding cuvettes without cuvette card left on stock.
-- To leave this function pres ESC key.
Known Problem
Table 9
Problem Possible solution
Default parameter setup could not be loaded.
Low Level setup has been loaded.
Display shows: -- Check actual parameter setup
universal default -- Import parameters from SD-Card
parameters loaded -- Check / calibrate thermal block temperature
-- Calibrate photometer
-- Check application parameters
-- Check / re-enter conversion parameters
Parameter function
Parameter export
This function is intended to export the instruments setup parameter to a
PAREXP.TXT file on SD-Card.
-- Press Enter key when < param. im/export > is displayed.
-- This below message will be displayed:
For detailed procedure please see chapter 3.9.3.

Introduction 23
Parameter import
This function is intended to import a parameter setup from PARIMP.TXT file on
SD-Card.
-- Press Enter key when < param. im/export > is displayed:
-- Press > key when < Import <- SD-Card > is displayed:
-- Press > key when < Import <- SD-Card > is displayed:
2.1.3 QUIT SERVICE MODE

It is always possible to exit the service mode when the selection of service func-
tion is displayed, just by pressing the ESC key. As shown below:
Press ESC key.
24
2.2 USER MODE

The user mode in HumaClot Junior is known as utilities menu, which can be ac-
cessed by the user as well as the service technician. In the user mode the instru-
ment can be adapted to the individual needs and the instrument parameters
can be printed.
2.2.1 UTILITIES MENU

The utilities menu is part of the installed software and each Operating Software
has pre-installed procedures into the Analyzer. You can refer to the User manu-
al for the pre-installed procedures.
2.2.2 ACCESS TO THE UTILITIES MENU

The utilities menu can be protected from any unauthorized access by a PIN code
to access the utilities menu follow the following steps:
-- Switch the Analyzer and the following messages will be displayed:
-- By pressing the > cursor key, the < UTILITIES > will be displayed:
-- Select < UTILITIES > by pressing the Mode key.

-- Enter the PIN code 1 1 1 1 1 (factory default), with the PIN code the utilities
menu can be protected against unauthorized access.
-- The functions of the utilities menu will be displayed, by pressing the cursors
keys you can select between the functions and confirm it with the Enter key.

Introduction 25
2.2.3 FUNCTIONS OF THE UTILITIES MENU

The UTILITIES menu has several functions; each function will be explained in the
following pages.
English Table 10
-- Off
-- On
-- AUTO
1 Printer -- Parameter-Protocol
• Global
• Actual Method
• All Parameter
-- Off
2 Port A
-- Ext. Printer
-- Off
3 Port B
-- Host
-- On
4 Beeper -- Off
-- Click
-- English
-- Deutsch
-- Espanol
5 Language
-- Francais
-- Portuges
-- Russian
6 Date/Time
-- on (= number)
7 PIN Code
-- off (= 0)
8 Cuvette-Test
-- Export -> SD-Card
9 Param. im/export
-- Import <- SD-Card
10 Photometer calibr.
26
Printer
-- When < Printer > is displayed, press the Enter key .
-- By pressing Enter key the selection will be activated and executed.

-- By pressing ESC key the selection will be executed.
Table 11
Display shows Explanation
DEFAULT
Off Disables printout to external Printer con-
nected to Port A
Enables manually started printout to
external printer connected at Port A
On
! Note: Port A has to be set to “ext.

printer”
Enables automatically started printout to
external printer connected at Port A
Auto

printer”
Printout function
Parameter Protocol
See next page
Parameter Protocol
-- By pressing the right cursor the function will be changed , when the function
parameter protocol is displayed press the Enter key.

Introduction 27

Initiates a printout of global parameters
Table 12
to external Printer connected to Port A
< Global >

printer”
Initiates a printout of Actual Method
parameters to external Printer connect-
< Actual Method > ed to Port A

printer”
Initiates a printout of All setup para-
meters to external Printer connected to
< All Parameter > Port A

printer”

Port A
-- When the function Port A is displayed, press Enter key.
-- This message below it will be displayed:

DEFAULT Table 13
< Off > Disables printout functions to an external print-
er connected to serial Port A
Enables printout functions to an external printer
< ext Printer >
connected to serial Port A


28
Port B
When the function Port B is displayed, press Enter key:
Table 14 Display shows Explanation

DEFAULT
< Off > Disables data output functions to an external
computer (Host) connected to USB Port B
Enables data output functions to an external
computer connected to serial USB Port B
Enables 5VDC supply for an external Barcode
Reader connected to serial Port A
< Host >
Enables Patient-ID functionality at HumaClot
Junior
! Note: Patient ID can entered either manually

by kepas or automatically by Barcode Reader

For detailed procedure please follow “LIS”.” described in LIS manual (LIS manual
available on request).
Beeper
When the function Beeper is displayed, press Enter key.

Introduction 29
Display shows Explanation Table 15

DEFAULT
< On >
Enables all beep tones.
< Off > Disables all beep tones.
< Click > Only key strokes will be confirmed by the beeper.

Language
In this function you will be able to change the language of the Analyzer expect
the language of the service menu, it will always be in English.
-- When the function Beeper is displayed, press Enter key:

DEFAULT Table 16
< English >
Set user interface to English language.
DEFAULT
< Deutsch >
Set user interface to German language.
DEFAULT
< Espanol >
Set user interface to Spanish language.
DEFAULT
< Francais >
Set user interface to French language.
DEFAULT
< Portuges >
Set user interface to Portugese language.
DEFAULT
< Russian >
Set user interface to Russian language.

30
Clock (date / time)

-- When the function (date / time) is displayed, press Enter key:
-- Set the date, it has to be entered in the following form:

DD <Enter> MM <Enter> YY <Enter>
-- Set the time, it has to be entered in the following form:

HH <Enter> MM <Enter> SS <Enter>
-- By pressing the Enter key the selection will be activated or executed.
PIN Code
With this function the user will be able to change the PIN Code for the access to
the utilities menu and the setup menu for test applications.
-- When the function PIN Code is displayed, press Enter key:

Table 17 DEFAULT
11111
Factory default PIN Code
! Note: If a printer is connected

and set to „AUTO“or „MANU-
AL“ any changes of the PIN Code
00000
Disables Requests for PIN Code for configura-
tions in UTILITIES menu and test applications
menu.
will be printed for reference. The range between 00001 and 59999 is allowed.
00001 to 59999
The code has always to be 5 digits
-- By pressing the Enter key the selection will be activated or executed.

Introduction 31
Cuvette test
With this function it is possible to check the cuvette detection.
-- When the function Cuvette test is displayed, press Enter key:

Function cuvette detection is not ready yet. Table 18
Wait until Analyzer has warmed up properly

??? Wait until Analyzer has reached “Standby” state
Process “Photometer check” in measurement
mode.
No Cuvette in measuring channel present.
---
No cuvette in measuring channel detected.
Cuvette in measuring channel present.
Cuv
Cuvette in measuring channel detected.
-- By pressing the ESC key this function will be executed. ! Note: Make sure that are no
cuvettes in the measuring
channels while the instrument is
If any problem occurred with the detection of the cuvette during a measuring switched on and that are no cu-
run or during this test menu remove all cuvettes from the measuring channels vettes in the measuring channels
and switch the instrument off and on again. Then recheck this function. while Photometer check.
32
Parameter export
With this function you will be able to export the instruments setup parameter
to a PAREXP.TXT file on SD-Card.
-- When the function Parameter export is displayed, press Enter key.
Parameter import
With this function you will be able to import the instruments setup from PA-
RIMP.TXT file on SD-Card.
-- When the function Parameter import is displayed, press Enter key:
-- Press > key when this below message is displayed:
-- Press > key when this below message is displayed:

Introduction 33
Photometer calibration
The HumaClot Junior is equipped with a 1 channel / 1 wavelength photome-
ter. This photometer is calibrated by factory. With this function it is possible to
re-calibrate the photometer.
-- When the function Photometer calibration is displayed, press Enter key:
-- Remove cuvette from measuring channel and Close Light Protection Cap.
Also Keep Light protection cap closed while processing.
-- Press Enter key to calibrate and the ESC key to leave this function.
2.2.4 QUIT UTILITIES MENU

To quit the utilities menus there are two cases:
Case1: If nothing was changed

-- Press ESC key when this message below is displayed:
OR
-- Press ESC key when this message below is displayed:
34
-- This message below will pop up:
-- Standby indicates system is ready to be used.
Case2: If there were a change in the UTILITIES setup

-- When this message below is displaced either by pressing the Enter key to
save the new parameter or by pressing the ESC key to quit without saving.
-- This below message will pop up for few seconds:
-- Standby indicates system is ready to be used.
2.2.5 PIN-CODE FORGOTTEN

If the User has forgotten the PIN-Code it is possible to reset the PIN-Code by
Service.- To reset the PIN-Code follow the following steps:
-- Switch on the Analyzer

Introduction 35
-- Select the < UTILITIES > by pressing the Mode key.
-- Key in „Superior Security Code“ (35001) and you will get an access to the
utilities menus.
-- Press > key until the function < PIN Code > is displayed and confirm it with
the Enter key.
-- Key in a new PIN-Code for example (11111).
-- Press ESC key when the below message is displayed:
-- When the message below is displayed, press Enter key to save:
-- STANDBY indicates system is ready to be used.

36
Table 19
PIN Codes Explanation
DEFAULT
11111
Factory default PIN Code
Disables Requests for PIN Code for configurations in
00000
UTILITIES menu and test applications menu.
The range between 00001 and 59999 is allowed. The
00001 to 59999
code has always to be 5 digits.
ONLY FOR SERVICE
35001 Fixed „Superior Security Code“ in case PIN code was for-
gotten.
Important:
The „Superior Security Code“ must be kept confidential and should not be made
accessible to third parties.

ADJUSTMENTS 37
3 ADJUSTMENTS
3.1 Repair Matrix
Adjust / Calibrate Software / Parameter / Setup Test Functions Table 20

Calibrate Thermalblock temperature
Check conversion parameters
Enter conversion parameters

Check Speed sensor distance
Adjust Display Contrast
Check Softwareversion
Calibrate photometer
Check Mixer spinning
Check Parameter-ID
Parameter update
Software update
Set Date / Time
Keyboard test
Display test
Mixer test
Optic test
Replacement:
LCD-Module X
PCB Mainboard X X X X X X (X) X (X) X X (X) X X X X
Power Supply
Keyboard Overlay X
Display Cable X X
Thermal Block X X X
Thermal Switch
Mixer Motor X X X
Battery X
Housing X X X X X X (X) X (X) X X (X) X X X X
Light Protection Cap
Software / Parameter
Software Update (X) (X) X X (X) X (X)
Parameter Update X (X) X (X) X X (X)
Default Param. Loaded (X) (X) X X
38
3.2 Service check

The HumaClot Junior needs a less service and maintenance action, so the fol-
lowing checklist will give suggested preventive checks for a proper function.
Type: ______________ Serial-No.: ______________

Software Version:______________ Parameter-ID.: ______________
Pos. OK nOK done

1 Identification
Check the rating plate on the bottom site (Type, Serial num-
ber) write down in protocol --- ---
2 Known Problems
Are there any known problems reported by the user?
3 Visual control
Is the outside of the Analyzer in good conditions?
Are there any parts to be replaced?
Is the measuring channel clean (free from samples, reagents,
particles)?
Is the light protection cap clean? Can it move freely?
Is the ext. power supply in good condition?
Is the cable free from damages?
Does the DC plug fit into DC socket of Analyzer?
4 Parameter protocol
Print parameter protocol w. external printer, check printout --- ---
Does type and serial number of printout match to the rating
plate?
Actual date and time on printout
Software version
Parameter-ID
5 Replacements
Replace Li-Battery after 4 years (± 1 year)
6 Technical check (use service menu)
Optic test < optic-test >
Mixer test < mixer-test > / mixer spinning
Keyboard test < keyboard-test >
Check / Calibrate thermal block temperature (37,4°C ±0,4°C)
Check / Calibrate photometer (if required)
7 Analytical check
Run QC-Test with aPTT in double determination with Control
plasma N and Control plasma P
Service Check: Passed Not passed

_________________ _________________ _________________
Date Name Sign

ADJUSTMENTS 39
Pos. Quantity Order-No. Description
Remarks:
3.3 LCD contrast

Adjusting the LCD contrast will lead to the best combination of a good visibility
of characters and less grey shadows in the background.
Preparation:
-- Switch ON the Analyzer, wait until Analyzer warms up.
-- Wait until Analyzer has passed temperature stabilizing countdown.
-- When STANDBY is displayed, that’s mean the system is ready to be used.

40
Adjustment:
-- From the back side of the HumaClot Junior you will notice a potentiometer
inside a small hole.
-- Use a small screwdriver for adjusting the LCD-contrast.
Figure 2
Adjusting the LCD MODULE
3.4 Speed sensor distance

For a proper speed sensor function make sure that the space between magnet
and speed sensor is larger or equal 3mm (≥ 3mm). As shown in the following
figure.
Table 21
Space between magnet
and speed sensor
≤ 3mm Speed sensor malfunction
3-4mm Optimal distance
≥ 4mm Speed sensor malfunction
Figure 3
Adjusting the Hall sensor

ADJUSTMENTS 41
3.5 Mixer spinning

A proper function of the mixer motor and the mixer is required for a save func-
tion. Follow the following procedures to get an objective visual picture of the
spinning mixer.
-- Prepare a new cuvette with 200µl distilled water. ( It is required to use a new
cuvette with a new mixer to get an objective visual picture of the spinning
mixer).
-- Make sure that a new mixer is inside.
-- Make sure that there are no air bubbles inside.
-- Start Analyzer in service mode.
-- Select <mixer >.
-- Start motor with 200 rpm.
-- Put prepared cuvette into measuring channel.
-- Make a visual check:
1. Does the mixer spin in the centre of the cuvette.
2.
3. *
Does the spinning mixer seems to be a star like ( ).
In case it seems to be a triangle like () then check or replace the mag-
net / mixer motor unit.
3.6 Check thermal block temperature

Preparation:
-- Prefill a defined cuvette position (1) of incubation block with (¾) distilled
water.
-- Put the distilled water direct into the block.
-- Do NOT use any cuvette therefore.
42
Figure 4
Prefill postion(1) of inctubation
block with distilled water
-- Place external temperature sensor (probe) in the water filled position (1).
Figure 5
An External Probe is placed in
position (1)
-- Switch ON the Analyzer and wait until the Analyzer warms up.
-- Wait until the Analyzer passes temperature stabilizing countdown.
-- When STANDBY is displayed that’s mean that the system has warmed up
successfully.
-- Read the value from the external Thermometer. If thermal block tempera-
ture is out of range refer to “thermal block calibration”.

ADJUSTMENTS 43
Target temperatures: 37,4°C

Tolerance: +/- 0,4 °C
Figure 6
-- When check process is completed, Remove the probe first then remove the
distilled water from incubation position.
Figure 7
3.7 Calibrate thermal block temperature

Preparation procedures:
-- Prefill a defined cuvette position (1) of incubation block with (¾) distilled
water put the distilled water direct into the block. Therefore, do NOT use any
cuvette.
Figure 8
44
-- Place external temperature sensor (probe) in the water filled position.
Figure 9
-- Switch on the Analyzer and key in the service code while the text line is
scrolling.
-- The service code is accepted.
-- Press the arrow keys until < calib. temperature > is displayed:
-- Press Enter key.
Calibration procedures:
1. Wait until temperature has stabilized to 37.4 and it will be displayed in
intern field.

ADJUSTMENTS 45
2. Read the actual temperature of thermal block from digital multimeter.

3. Key in the actual temperature and it will be displayed in the extn field auto-
matically, the decimal point can be entered by pressing the > Key and press
the Enter key to confirm. The calibration value will be set in the Analyzer
automatically and it will be displayed automatically.
4. Wait again until the temperature has stabilized to 37, 4 in the intern line.
5. If the values deviate repeat the procedure from step 2, till the both values
are the same then you will be able to finish the calibration and save it by
pressing the ESC key.
6. After pressing ESC key following display will appear:
7. Leave the service functions by pressing the ESC key.

8. Wait until the parameters are checked.
9. Press Enter key for saving the new temperature calibration.
10. Thermal block calibration has finished now, remove probe and remove the
distilled water from the incubation position.
46
Figure 10

ADJUSTMENTS 47
3.8 Software update

To update the software of the HumaClot Junior a certain hardware and software
requirements are required:
Hardware Requirements:
-- SD-Card:
• Any SD-Card more than 32GB won’t work; also any SD-XD won’t work.
• The Acceptable Storage capacity for the SD-card is (128MB, 256MB,
512MB, 1 GB, 2 GB, 4 GB, 8 GB, 16 GB, and 32 GB).
-- CARD-Reading unit for PC
-- Desktop PC or Notebook if SD-Card is not prepared
-- Digital Photo Camera if SD-Card is not prepared
Software requirements (if SD-Card is not prepared):

-- Operating System (Windows 2000, Windows XP, Windows Vista, Windows7,
Windows 8, Windows 10).
-- PC Software (SD formatter V4.0 or later, https://www.sdcard.org/down-
loads/formatter_4/)
-- Software file for HumaClot Junior update (1-Channel Software)
-- Data file for parameter update (PARIMP.TXT)
General File structure on SD-Card
Type SD-Card Description Note Table 22

Root-Directory <ROOT> Filesystem: FAT
Filesystem: FAT32
Folder CD12BOOT
HC1_01v00.MX 1-Channel SW
File
File
File
Folder CD12DATA
File PARIMP.TXT
48
1. Formatting SD-Card:
For HumaClot Junior it is important to prepare the SD-Card at first.
Make sure that the file system is FAT or FAT32, Other file systems as NTFS
and others will not be accepted by the Analyzer. To format an SD-Card we
suggest using special software called SD Formatter which is provided by SD
Association www.sdcard.org or a digital photo camera. When the SD-cards
are formatted directly under windows or Linux operating systems will not be
accepted by the Analyzer.
In case of a strong fragmentation of the SD-card, it may be possible that the

Analyzer will suddenly not accept the card anymore. In this case the SD-card
needs to be formatted by a digital photo camera. Also defragmentation or
formatting with Windows or Linux operating systems is not acceptable by
the Analyzer.
For software update a special file- and folder structure is required, also it is
not allowed to have more than one pcs of *.MX file in CD12BOOT folder
at the same time.please follow the following procedures to update the soft-
ware:
Table 23
Step Action Folder-/ Filename Target
1 Create folder CD12BOOT SD-Card <ROOT>-Directory
2 File copy HC1_01v00.MX SD-Card CD12BOOT folder
-- Create a folder in the root of your SD-card called (CD12BOOT).
Figure 11
-- For updating the 1-Channel, select and copy the folder (HC1_01v00.mx) into
the folder (CD12BOOT).
Figure 12
-- Pick the prepared SD-Card for software update; make sure that the SD-Card
is placed on the “lock” position. This prevents any information on the card
from being altered or deleted.

ADJUSTMENTS 49
Figure 13
-- The write protection will enable the Boot-Loader routine in the Analyzer
software.
Figure 14
-- Turn Analyzer upside down.
Figure 15
-- Insert SD-Card into SD-Card reading unit.

50
Figure 16
-- Insert SD-Card completely and a “click” tone will be heard when the SD-card
is inserted completely.
Figure 17
-- Turn the Analyzer back to original position, and turn on the Analyzer by plug-
ging the power supply.
Figure 18

ADJUSTMENTS 51
-- The display shows activated Boot-Loader. If not refer to “known problems”

in the following pages.
-- Wait until the programming new software version is displayed:
-- Wait until the programming process is finished.
-- Switch OFF the Analyzer.

-- Remove the SD-Card.
-- Switch ON the Analyzer.
Known Problems:
Problem Possible solution Table 24

-- Check write protection of SD-Card is activated
-- Check folder structure on SD-Card
-- Check folder name
The BOOT-Loader does not
the folder name must written exactly as in-
start
Display shows not structed
BOOT-Loader screen - - Check file structure on SD-Card
-- Check file system FAT or FAT32 on SD-Card
format SD-Card with SD Formatter Software
in case use different photo camera model.
Wrong Analyzer software -- Check installation software file on SD-Card
installed
Wrong software version -- Check installation software file on SD-Card
installed
52
Update passes 2 times

-- Check installation software on SD-Card
programming proc.
make sure that only one software-.MX-File is
Programming Applic. is
in folder.
shown twice
-- Refer to special instruction for software
update
All other case
possibly special actions are requested.
Check of additional documentation.
3.9 Parameter Update
3.9.1 PARAMETER IMPORT

For importing a certain hardware and software requirements are required:
-- SD-Card:

Windows 8, Windows 10¬).
loads/formatter_4/)
-- Software file for HumaClot Junior update (1-Channel Software).
-- Data file for parameter update (PARIMP.AUT)

ADJUSTMENTS 53
General Filestructure on SD-Card

Filesystem: FAT32
Folder CD12BOOT
File
File
File
Folder CD12DATA
File PARIMP.TXT
For HumaClot Junior it is important to prepare the SD-Card at first, make
sure that the file system is FAT or FAT32.Other file systems as NTFS and oth-
ers will not be accepted by the Analyzer. To format a SD-Card we suggest us-
ing special software called SD Formatter which is provided by SD Association
www.sdcard.org or a digital photo camera.
Also SD-Cards directly formatted under Windows or Linux operating sys-

tems will not be accepted by the Analyzer. When the SD-cards are formatted
directly under windows or Linux operating systems will not be accepted by
the Analyzer. In case of a strong fragmentation of the SD-card, it may be
possible that the Analyzer will suddenly not accept the card anymore. In this
case the SD-card needs to be formatted by a digital photo camera.
Also defragmentation or formatting with Windows or Linux operating sys-

tems is not acceptable by the Analyzer. For a parameter update a special
file- and folder structure is required. Also it is not suggested to have more
than one pcs of *.TXT or PARIMP.* file in CD12DATA folder at the same time.
Please follow the following procedures to update the parameter:
Step Action Folder-/ Filename Target Table 26

1 Create folder CD12DATA SD-Card <ROOT> - Directory
2 File copy PARIMP.TXT SD-Card CD12DATA folder
54
-- Create a folder in the root of your SD-card called (CD12DATA)
Figure 19
-- Copy the parameter file (PARIMP.TXT) and paste in the previous folder
(CD12DATA).
Figure 20
Figure 21
software.
Figure 22

ADJUSTMENTS 55
Figure 23
Figure 24
Figure 25
56
Figure 26
-- Switch on the Analyzer and wait until the start up procedure has passed.
-- Select < UTILITIES > by pressing Mode key.
-- Key in PIN code 1 1 1 1 1 (factory default).
-- Select < Param. im/export > by pressing Enter key.
-- By pressing the cursor key, select < Import <- SD-Card > by pressing Enter key.

ADJUSTMENTS 57
-- Parameter import is in process. Wait approx. 10 seconds.
-- Parameters have been imported, press Esc key to save the new parameters.
-- Press Enter key for saving the new Parameters.
-- New parameters have been installed now.
-- Remove SD-Card.
-- Printout parameter-protocol “global”.
-- Check Parameter-ID.
-- Check thermalblock temperature.
-- Check calibration (conversion) parameters.
58
Known Problems:
Table 27
Display message
BOOT-Loader V1.00
No application or firmware -- Reset write protection of SD-Card (switch)
update on the memory
card! Remove card.
the folder name must written exactly as in-
Display message structed
Couldn´t open ´parimp.txt´ -- Check file structure on SD-Card
-- Check file system FAT on SD-Card
format SD-Card with different photo camera
model.
Display message
-- No valid parameter in line x
WARNING Line : ´xxxxxx´
-- Press Enter to over jump message and proceed
ignored
-- Check parameters after import procedure
ENTER= continue
Wrong parameter version
-- Check installation parameter file on SD-Card
installed
-- Refer to special instruction for parameter up-
date
All other cases
possibly special actions are requested.
3.9.2 AUTOMATIC PARAMETER IMPORT

For importing Automatic Parameter, a certain hardware and software require-
ments are required:
-- SD-Card:

ADJUSTMENTS 59

-- Operating System (Windows 2000, Windows XP, Windows Vista, Windows
7, Windows 8, Windows 10).
loads/formatter_4/)
-- Data file for parameter update (PARIMP.TXT).

Filesystem: FAT32
Folder CD12BOOT
File
File
File
Folder CD12DATA
File PARIMP.TXT


60
Table 29
1 Create folder CD12DATA SD-Card <ROOT>-Directory
2 File copy PARIMP.AUT SD-Card CD12DATA folder
-- Create a folder in the root of your SD-card called (CD12DATA).
Figure 27
-- Copy the parameter file (PARIMP.TXT) and paste in the previous folder (CD-
12DATA).
Figure 28
Figure 29
software.

ADJUSTMENTS 61
Figure 30
Figure 31
Figure 32
62
Figure 33
Figure 34
-- Switch on the Analyzer and these below messages will be displayed:
-- The parameter import automatically will start.

ADJUSTMENTS 63
-- Parameter import is in process, please wait for a few seconds.
-- When this message below is displayed, that’s mean that the parameter have
be installed:
-- Remove SD-Card.
-- Remove PARIMP.AUT file from SD-Card.
-- Printout parameter-protocol “global”.
-- Check Parameter-ID.
-- Check thermal block temperature.
-- Check calibration (conversion) parameters.
Known Problems:

Display message
BOOT-Loader V1.00
update on the memory
card! Remove card.
The folder name must written exactly as
No automatic parameter instructed
import -- Check file structure on SD-Card
Format SD-Card with different photo camera
model.
Display message
-- No valid parameter in line x
WARNING Line: ´xxxxxx´
-- Press Enter to over jump message and proceed
ignored
-- Check parameters after import procedure
ENTER= continue
-- Check installation parameter file on SD-Card
installed
64
Automatic parameter
import every startup
After switching on Ana- -- Remove SD-Card
lyzer a parameter import -- Remove PARIMP.AUT file from SD-Card
starts automatically
-- Refer to special instruction for parameter up-

date
All other cases
Possibly special actions are requested.
3.9.3 PARAMETER EXPORT

For importing a certain hardware and software requirements are required:
-- SD-Card:

Windows 8, Windows 10¬).
loads/formatter_4/)
-- Data file for parameter update (PARIMP.TXT)

ADJUSTMENTS 65
General Filestructure on SD-Card

Filesystem: FAT32
Folder CD12BOOT
File
File
File
Folder CD12DATA
File PARIMP.TXT



1 Create folder CD12DATA SD-Card <ROOT>-Directory
-- Create a folder in the root of your SD-card called (CD12DATA).
Figure 35
66
Figure 36
software.
Figure 37
Figure 38

ADJUSTMENTS 67
Figure 39
Figure 40
Figure 41
-- Switch on the Analyzer and wait unit the start up procedure has passed.
68
-- Select < UTILITIES > by pressing Mode key.
-- Key in PIN cod 1 1 1 1 1 (factory default).
-- Select < Param. im/export > by pressing Enter key.
-- When < Export -> SD-Card > is displayed press Enter key:
-- Parameter export is in process. Please Wait approx. 10 seconds.
-- Parameters have been exported, press ESC key.
-- Wait until the parameters are checked.

ADJUSTMENTS 69
-- The export parameter fill will be stord on the SD-Card.
-- Switch OFF Analyzer.

-- Remove SD-Card.
-- Switch ON Analyzer.
-- Check if PAREXP.TXT file exists.
-- On SD-CARD.
Known Problems:

SD-Card is write protected
-- Reset write protection of SD-Card
Display shows SD-Card is write-pro-
(switch)
tected
-- Analyzer will ceate folder by itself
-- Make sure that there is enough
No Folder CD12DATA on SD Card free memory on SD-Card
-- Format SD-Card filesystem as FAT
or FAT32
-- Analyzer will ceate folder by itself
-- Make sure that there is enough
No file PAREXP.TXT on SD-Card free memory on SD-Card
-- Format SD-Card filesystem as FAT
or FAT32
70
3.9.4 PARAMETER ERROR

The parameter setup of the Analyzer is stored in an internal EEPROMT and the
system is checking the parameters with checksum. In case of a checksum error
the HumaClot Junior will display a “Parameter Error” and will stop working. The
following description guides to handle this case in order to reload the factory
setup.
-- This below message will be displayed, press Enter key (“weiter”: means con-
tinue):
-- When this message below is displayed, press Enter key:
-- When this message below is displayed, press 7 key:
-- When this message below is displayed, press Enter key (“weiter” means con-
tinue):

ADJUSTMENTS 71
-- The factory default parameters have been loaded back to EEPROM.

-- Check / calibrate thermal block temperature
-- Load new cuvette balance
-- Contact distributor regarding cuvettes without cuvette card left on stock.
Known Problems:

Default parameter setup could not be loaded.
Low Level setup has been loaded.
-- Check actual parameter setup
Display shows:
-- Import parameters from SD-Card
universal default
-- Check / calibrate thermal block temperature
parameters loaded
3.10 Parameter protocol

The printout of the protocol parameter is an overview of the structure of the cur-
rent Analyzer (example). Regarding the size of a complete parameter protocol
the Analyzer offers the possibility to printout separate sections:
1. Global
2. Actual method
3. All Parameter
72
Printout global
-- Wait until the start procedures ends.
-- Key in the PIN code 1 1 1 1 1 (factory default).
-- Press Enter key when < Printer > is displayed:
-- By pressing the arrow keys, select < Parameter-Protocol > by pressing Enter
key.
-- Press Enter key when < Global > is displayed and printout will start right now
(See example 1 in the following pages):

ADJUSTMENTS 73
-- Press ESC key.

74
Figure 42

ADJUSTMENTS 75
Printout Actual Method

-- By pressing the arrow keys, select < Parameter-Protocol > by pressing Enter
key.
-- Press Enter key when < Actual Method > and printout will start right now
(see example 2 in the following pages )
76
-- Press ESC key.

ADJUSTMENTS 77
Example2: Actual method printout
Figure 43
78
Explanations about Example 2:
Mixer Setup:
Figure 44
Case1: onstant speed

Mixer:
rpm1 = 500
rpm2 = 500
rpm1->2 = 10 s
Figure 45
Case 2: Reduced speed

Mixer:
rpm1 = 400
rpm2 = 200
rpm1->2 = 10 s
Clotting (pre phases and measurement):
Case 1: lag > adjust + learn

Figure 46 adjust = 2 s (B)
learn = 3 s (C)
lag = 8 s (A)
Sensitive for clot detec-

tion after 5 sec. until max.
meas. Time =
< Meas. time > sec. (D)
-- adjust is fixed
pre-set by 2 sec.

ADJUSTMENTS 79
Case 2: lag = adjust + learn

adjust = 2 s (B)
learn = 3 s (C)
Figure 47
lag = 5 s (A)

meas. Time =
< Meas. time > sec. (D)
-- adjust is fixed pre-set by

2 sec.
Case 3: lag < adjust + learn

adjust = 2 s (B) Figure 48
learn = 3 s (C)
lag = 1 s (A)

meas. =
< Meas. time > sec.
-- adjust is fixed pre-set by

2 sec.
All Parameters
Printout all parameters:

80
-- Select < Paramete-rProtocol > by pressing the arrow keys, and confirm it with
Enter key.
-- Press Enter key when < All Parameter > and printout will start right now (See
Example 3 in the following pages).
-- Press ESC key.

ADJUSTMENTS 81
Example 3: all parameters printout:
Figure 49
82
3.11 Printer connection

The HumaClot Junior can be connected to an external serial thermal printer,
Human GmbH suggest using Seiko Epson DPU-414 thermal printer. To connect
the DPU-414 printer with the HumaClot Junior a special cable is required (See
chapter 6). Before using the printer with HumaClot Junior, the serial port of the
printer must be configured.
Figure 50
DPU-414 printer
Follow the following procedures to connect the HumaClot Junior with an exter-
nal printer:
-- Connect the printer cable to the serial port connector of the Analyzer.
Figure 51
-- When the Analyzer is connected to an external printer the results can be

printed automatically.

ADJUSTMENTS 83
Figure 52
Setup:
Port A: <ext. printer>
Port B: <Off
-- The printout at the Analyzer has to be enabled at first.

-- Select < Auto > by pressing the arrow keys, and confirm it with Enter key.
84
-- Press Enter key.
-- When < Port A > is displayed confirm it with Enter key:
-- By pressing the arrow keys, select < ext. Printer > and confirm it with Enter
key.
-- Press ESC key.

ADJUSTMENTS 85
-- Press Enter key to save the new parameter setup.
3.12 DPU 414 printer setup

Print a list of current setting:
1. Slide the power switch to ON while pressing the ON/OFFLINE/PAPER END
button.
2. Red and green lamps blink alternately.
3. Release the button after a list of current settings starts printing out (as
shown in the following table).
-- For changing the setting of the Dip Switches, please refer to the printer’s
user manual.
Figure 53
86
Set Up the thermal printer DPU-414

This section describes how to change the settings of the Dip switches of the
Thermal Printer DPU-414 with the use of the buttons ONLINE and FEED.
Button: ONLINE Dip Switch = ON

Button: FEED Dip Switch = OFF
Set the status of a Dip switch to “ON” by pressing ONLINE button or to “OFF” by
pressing FEED button as indicated in the tables below. After the ONLINE button
has been pressed the GREEN lamp indicates that the button operation has been
confirmed. When the FEED button has been pressed, the button operation is
confirmed by the RED lamp.
Alternately blinking of both lamps indicates that the new settings are written
to memory.
WARNING: Never turn the printer OFF while it is writing new settings to mem-
ory.
After each Dip switch setting via button, the status of the actual Dip switch is
printed out automatically. Wait until “DIP SW setting complete!!” is printed out
and the printer returns to the ONLINE mode before turn off the power.
Procedure
Table 34 Step Action Lamps Printer prints:

1 Slide the power switch OFF
Slide the power switch RED and GREEN
Starting to print
2 ON while pressing ONLINE lights blinking
out
button alternately
Release the button after
3 a list of current settings
starts printing out
Continue?: Push
The print out of the current “On-line-SW”
3
settings is followed by: Write?: Push
“Paper feed SW”
Move to chart Dip SW-1
4
Next page

ADJUSTMENTS 87
DIP SW -1
Step Action Lamps Printer prints: Table 35

5 Press ONLINE button. RED lamp lights Dip Switches-1
1 (OFF): Input =
6 Press FEED button. GREEN lamp lights
Serial
2 (ON): Printing
7 Press ONLINE button. RED lamp lights
Speed = High
3 (ON): Auto
Loading = ON
4 (OFF): Auto LF =
9 Press FEED button. GREEN lamp lights
OFF
5 (ON): Setting
Command = Enable
11 Press FEED button. GREEN lamp lights 6 (OFF): Printing
12 Press ONLINE button. RED lamp lights 7 (ON): Density
8 (ON): 100%
Continue?:
13 Press ONLINE button. RED lamp lights Push “On-line-SW”
Write ?: Push
“Paper feed SW”
14 Press ONLINE button.
15
Next page
DIP SW -2
Step Action Lamps Printer prints: Table 36

With Dip Switches-2 show-
ing on the printer paper
1 (ON): Printing
Colums = 40
2 (ON): User Font
Back-up = ON
3 (ON): Character
Select = Normal
4 (ON): Zero =Nor-
mal
5 (ON): Internatio-
nal
21 Press ONLINE button. RED lamp lights 6 (ON): Character
22 Press ONLINE button. RED lamp lights 7 (ON): Set
88
8 (OFF): = USA
Continue?:
23 Press FEED button GREEN lamp lights Push “On-line-SW”
Write?: Push
“Paper feed SW”
24 Press ONLINE button.
25
Next page
DIP SW -3
Table 37
Step Action Lamps Printer prints:
With Dip Switches-3 show-
ing on the printer paper
1 (ON): Data Length
= 8 bits
2 (ON): Parity Set-
ting = No
3 (ON): Parity Con-
dition = odd
4 (ON): Busy Con-
trol = XON/XOFF
30 Press FEED button GREEN lamp lights 5 (OFF): Baud
31 Press ONLINE button. RED lamp lights 6 (ON): Rate
32 Press ONLINE button. RED lamp lights 7 (ON): Select
8 (ON): = 9600 bps
Continue ?: Push
“On-line-SW”
Write ?: Push “Pa-
per feed SW”
RED and GREEN

lights blinking
alternately Dip Switch settings
34 Press FEED button
complete!
GREEN lamp lights
permanent
35 Slide the power switch OFF

ADJUSTMENTS 89
3.13 Load CuvetteCARD

The HumaClot Junior is equipped with a CuvetteCARD system.
Figure 54
Right view of HumaClot junior
-- Select STANDBY -Mode.
-- The CuvetteCARD reading unit is placed on the right side of the Analyzer.
Figure 55
-- Insert the chip end of the CuvetteCARD into the Card reader Port.
Figure 56
90
-- When the CuvetteCARD is fully inserted into the Analyzer, it will identify a
valid cuvette card by:
• Showing a valid cuvette credit  bookable : > 500 <
• Entering a quantity at  debit: > 500 <
-- By pressing the Enter key the number of cuvettes will be loaded to the Hu-
maClot Junior, but we suggest loading fully 500 cuvettes in one step to the
Analyzer.
-- Wait until this below message is displayed (it may take up to 20 seconds).
-- When this message below is displayed, remove the CuvetteCARD from the
reading unit. (Keep CuvetteCARD and save it if there is a credit of cuvettes
left on the card.)
-- After loading the cuvettes to the HumaClot Junior an automatic printout will
appear when the printer is connected.

ADJUSTMENTS 91
========================
========================
CuvCARD balance
loaded to
HumaClot Junior
V01.00
SerNo. M1360008
Date/Time:
17.10.2017, 08:22:27
-- CuvCARD Info --
Lot Number =’12345A67’
Balance = 500
CuvCARD devalued.
========================
========================
Known Problems:

CuvetteCard inserted in wrong direc-
Display shows:
tion
Warning: Please remove ChipCard
-- Remove Card, press Enter key, retry
Enter: continue
as instructed
Display shows: CuvetteCard already devaluated
Warning: Please remove ChipCard -- Remove Card, press Enter key retry
Enter: continue a different Card
Display shows: This card is not a CuvetteCARD
Warning: Please remove ChipCard -- Remove Card, press Enter key, retry
Enter: continue a CuvetteCARD
There is a remaining balance of “n”
cuvettes still left on the HumaClot
Display shows:
Junior
Remaing.balan.= 500
-- Check for existing cuvettes in
< keep >
stock if available remove
CuvCARD, press ESC key.
92
There is a remaining balance of “n”

cuvettes still left on the HumaClot
Display shows: Junior
Remaing.balan.= 500 -- Check for existing cuvettes in stock
< keep > if not available select < overwrite >,
press Enter key, continue proceed-
ing
While start up procedure of Huma-
Clot Junior the system indicates that
Display shows:
100 cuvettes or less are available.
Cuvette balance:
-- Check for further cuvettes and
100 measurements
CuvetteCARDs on stock.
-- Order new cuvettes
Wrong cuvette type
Display shows: -- Remove Card, press Enter key,
Warning -- Use matching cuvettes / Cuvette
Wrong cuvette type CARD
-- Contact service
Wrong distributor ID code
Display shows:
-- Remove Card, press Enter key,
Warning
-- Contact distributor
Invalid customer ID
-- Contact service
Only <100 cuvettes left on HumaClot
System loads cuvettes directly from Junior.
card without showing remaining System loads new volume automati-
balance. cally from inserted CuvCARD without
user communication
Cuvette Balance fully used
Printout Message:
Balance fully used! Cuvette volume used.
Only xx reserve measurm. -- Check for further cuvettes and
remain possible! CuvetteCARDs on stock.
======================== -- Order new cuvettes
*** Please *** -- Proceed by loading new cuvette
*** order new *** volume
*** Cuvettes ***
========================

ADJUSTMENTS 93
Balance fully used

Printout Message:
Cuvette volume completely used.
Balance and reserve
fully used.
========================
*** Please ***
-- Proceed by loading new cuvette
*** order new ***
volume
*** Cuvettes ***
========================
Cuvette volume completely used.
Display shows:
Locked!
Insert new CuvCard
-- Proceed by loading new cuvette
volume
3.14 Photometer calibration

The HumaClot Junior is equipped with a 1 channel / 1 wavelenght photometer.
this photometer is calibrated by factory.
In case a new calibration is required the please follow this procedure:
-- Switch ON the Analyzer and wait until the Analyzer warm up
-- Wait until the Analyzer passes temperature stabilizing countdown.
-- When STANDBY is displayed that’s mean that the system has warmed up
successfully and ready to use.
-- Check thermal block temperature as described in this chapter, if thermal

block temperature is OK, please follow the Photometer calibration proce-
dure (We suggest to do a printout of parameter protocol “global” before
calibrating).
94
-- Wait at least 1 hour in order to stabilize temperature in the whole Analyzer.

-- Select < photometer calibr > by pressing the arrow keys, and confirm it with
Enter key.
-- When this message below is displayed remove the Cuvette from the meas-
uring channel,close the light protection cap and keep the cap closed while
processing then press Enter key:
-- Press Enter key.
-- Make sure to keep the cap closed while processing.
-- If printer is connected a printout message will appear.

ADJUSTMENTS 95
------------------------
channel 1
------------------------
--- wavelength 405nm:
previous calibr. values
LED = 160
m-bright = 56518
r-bright = 42079
m-dark = 0
r-dark = 0
ext. gain = OFF
new calibration values

LED = 160
m-bright = 55448
r-bright = 41971
m-dark = 0
r-dark = 0
ext. gain = OFF
-- By pressing ECS key you will be able to finish the calibration.
-- Press ESC key.
-- Press Enter key to save the new parameter setup.

96
-- When this message below is displayed that’s mean that the new photome-
ter calibration have been saved.
Known Problems:
Table 39
Display shows: Preparation procedure has not been followed
PHOTOMETER CALIBRAT. correctly.
WARM UP 10:27 -- Start process from beginning
Calibration procedure failed.
-- Keep printout saved
Display shows:
-- Check for cuvettes in the measuring channel
Bright calib failed!
-- Remove cuvette from meas. Channel
405nm LED ENERGY LOW
-- Retry calibration process by pressing Enter key
-- Contact Human s service
Calibration procedure failed.
Display shows:
-- Check for dust/dirt in the measuring channel
-- Clean measuring channel
-- Retry calibration process by pressing Enter key
3.15 Virtual ComPort driver

The HumaClot Junior can be connected to a Computer (Host/LIS System) via
USB; the Analyzer would be able to detect an Virtual ComPort by installing the
matching driver.
Known Problems:
Table 40
Problems with STM Driver installation.
At Windows the USB is Unzip the file, run the executable, and then go to
detected as C:\Program Files (x86)\STMicroelectronics\Soft-
STM32 Virtual ComPort in ware\Virtua¬l comport driver in Windows Ex-
FS Mode plorer and double-click either dpinst_amd64.exe
for 64 bit systems, or dpinst_x86.exe for 32 bit.

INSTRUMENT ERRORS 97
4 INSTRUMENT ERRORS

-- Check mains power
suggestion: wait approx.. 15 minutes after
mains power returns to be sure that AC mains
The Analyzer does not is stable again.
start -- Check power connections
-- Check power supply 12VDC
Cannot start Analyzer Change power supply
-- Check internal fuse on PCB mainboard
-- Check PCB mainboard
-- Change PCB mainboard
suggestion: wait approx.. 15 minutes after
is stable again.
Analyzer fails during -- Check power connections
operation -- Check power supply 12VDC
-- Change power supply
The display shows no
suggestion: wait approx. 15 minutes after
characters
is stable again.
There are no beep tones
-- Check power connections
by pressing keyboard
-- Check power supply 12VDC
Change power supply
There are no beep tone
while starting up
characters
-- Check display contrast
There are single beep
tones while starting up
98
-- Check display cable inside Analyzer

-- Change display cable
characters
-- Check LCD-Module (display)
-- Change display
There is a continuous
beep tone every second
-- Recalibrate thermal block temperature
-- Check thermal block Connector to PCB Main-
board
-- Check thermal block heating
The Analyzer does not
-- Check thermal block sensor (100KΩ at 25°C)
heat up (Thermal Block)
-- Check thermal switch (for overtemp. protection)
-- Change thermal switch
-- Change thermal block
-- Change PCB Mainboard
-- Check thermal block Connector to PCB Main-
The Analyzer gets very
board
hot
-- Check thermal block sensor (100KΩ at 25°C)
(Thermal Block)
-- Check if Analyzer is placed in direct sunlight
COOL DOWN -- Check if Analyzer is placed next to a heating
-- Check present thermal block temperature with
Thermal block is too external thermometer
warm. -- Recalibrate thermal block temperature
-- Check thermal block connector to PCB Main-
Thermal block tempera- board
ture calibration value is -- Check thermal block sensor (100KΩ at 25°C)
not valid. -- Change thermal block
WARN: temp instable
-- Check if Analyzer is placed in direct sunlight
If during a measurement -- Check if Analyzer is placed next to a heating
the temperature of the -- Check present thermal block temperature with
thermal block deviates external thermometer
significantly from set -- Recalibrate thermal block temperature
value, the measurement -- Check thermal block connector to PCB Main-
is not cancelled. board
Instead this warning ap- -- Check thermal block sensor (100KΩ at 25°C)
pears in the display and -- Change thermal block
is also printed out via the -- Change PCB Mainboard
active printer.


-- Check if keyboard is damaged
-- Check if keyboard is connected properly
(inside Analyzer)
No correct Keyboard func-
-- Check if keyboard cable is damaged
tion
(inside Analyzer)
-- Change keyboard

Date and time setup are -- Check date and time in UTILITIES menu
not OK -- Change PCB Mainboard
Date and time setup are
-- Check date and time in UTILITIES menu
not OK
-- Replace battery
When Analyzer was
switched off

-- Check if printer is switched ON Table 44
-- Check if printer is ONLINE

-- Check printer connection (cable connection)
No automatic printout
-- Check Analyzer setup
Results should be printed
printer = Auto
when clotting is found
Port A = ext. Printer
-- Check correct setup of external printer
-- Check Port A interface (service menu)
-- Check if printer is switched ON
-- Check if printer is ONLINE
No manual printout -- Check printer connection (cable connection)
Results should be printed -- Check Analyzer setup
when displayed and Start- printer = Auto
key is pressed Port A = ext. Printer
printer = Auto
Printer prints “crazy”
Port A = ext. Printer
characters
100
Table 45
-- Check if LIS system is ONLINE
-- Check cable connection
No LIS data output
-- Check Virtual ComPort driver on computer
Results should be send
when clotting is found
Port B = Host
-- Check correct setup of LIS System
-- Check Port B interface (service menu)
Table 46 Problem Possible solution

No Patient-ID from -- Check barcode reader is powered on
Barcode -- Check cable connection to Port A
Patient ID should be -- Check Analyzer setup
read from Barcode when Port B = Host
requested by software -- Check for correct setup of barcode reader
indication -- Check Port A interface (service menu)
Problem /
Table 47 Possible solution
Display message
Parameter error!
Set to defaults -- See chapter 3.9.4
Parameter Error detected -- Contact Human service
in EEPROM memory
ERROR: xxx -- Contact Human service
-- Contact Human service
ERROR recursive
Problem /
Display message
break time -- Check for correct pipetting sample and reagent
The measuring time -- Check for existing clot formation inside
reached cuvette
“max. measuring time” -- Check for correct test application in setup
-- Check for clear fluid inside cuvette
-- Check for direct ambient light
break top
from lamp or direct sunlight
Exceeded measuring
-- Close light protection cap
range is too low
-- Change Analyzers placement
-- Check for air bubbles inside cuvette


-- Check for correct test volume inside cuvette
-- Check for pipetting direct into cuvette
rinsing fluid on cuvette wall is not allowed
break drift -- Check for a clean measuring chamber
Measuring signal drifted -- Check for foreign parts in bottom of measuring
after adding start reagent channel (example: mixer-bar)
break adjust -- Check for a clean measuring chamber
Light value is too low -- Check for foreign parts in bottom of measuring
while adjustment channel (example: mixer-bar)
break noise -- Check for a clean measuring chamber
Measuring Signal is too -- Check for foreign parts in bottom of measuring
noisy. channel (example: mixer-bar)
102
Table 49
Problem /
Possible solution
Display message
break
Measurement cancelled
n/a
by user with ESC + Start
Key
bright value failed
-- Check for cuvette inside measuring channel
Measuring signal too low
remove cuvette inside measuring channel
while Photometer check
-- Check for a clean measuring chamber
procedure.
-- Check for foreign parts in bottom of measuring
Measuring signal too low
channel
while Photometer check
procedure.
Calibrated photometer
bright value could not be
found.
-- Check of dirt in measuring channel
-- Check of dirt on magnet (inside Analyzer)
-- check if parts at magnet block the motor
break motor
example: mixing-bar
Mixer Motor or motor
-- Check if motor is spinning in service mode
speed sensor is not
-- Check if speed sensor is detecting correctly
working properly.
-- Check distance magnet vs. sensor
-- Change motor
Table 50
Problem /
Possible solution
Display message
Self starting -- Repeat startup process, make sure there is no
measurement cuvette inside measuring channel, keep light
Incubation timer starts protection cap closed while processing
without cuvette -- Calibrate Photometer, see chapter 3.14
Self starting measurement
Incubation timer starts
-- Repeat startup process, make sure there is no
without cuvette
cuvette inside measuring channel, keep light
protection cap closed while processing
-- Calibrate Photometer, see chapter 3.14


-- Check for foreign parts in bottom of measuring
Self starting
channel (example: mixer-bar)
measurement
Measuring timer starts -- Check for direct ambient light
without adding start from lamp or direct sunlight
reagent -- Close light protection cap
-- Repeat startup process, make sure there is no
cuvette inside measuring channel, keep light
protection cap closed while processing
-- Calibrate Photometer, see chapter 3.14

-- Aspirate fluid with a pipette out of the channel
-- Disassemble Analyzer
-- Clean thermal block with alcohol and fleece
Liquid has pipetted direct
-- Clean optics on PCB with alcohol and fleece
into measuring channel
carefully
There was no cuvette
-- Clean magnet with alcohol and fleece
inside before pipetting
-- Check / adjust distance between magnet and
speed sensor
-- Reassemble Analyzer
104
Table 52
Problem /
Possible solution
Display message
-- Check conversion parameters
ERR over -- Check calibration curve
Calculation overflow -- Check application setup
ERR div0 -- Check calibration curve
Calculation error -- Check application setup
-- Remove cuvette and check if a clot is visible
ERR no clot inside cuvette
No clotting detected dur- -- Check application setup
ing measurement -- Repeat measurement
ERR log0 -- Check calibration curve
Calculation error -- Check application setup
min-value reached- -- Check range of calibration
no more points -- Check conversion parameters
While input of calibration -- Check calibration curve
curve minimum value is -- Check application setup
reached. -- Contact Human service
max-value reached- -- Check range of calibration
no more points -- Check conversion parameters
While input of calibration -- Check calibration curve
curve maximum value is -- Check application setup
reached. -- Contact Human service
Problem /
Table 53
Possible solution
Display message
(Channel) locked
The measuring channel
is locked due to no re- -- Load new cuvette balance to the Analyzer
maining cuvette balance
available
CuvCARD read error -- Check for correct CuvetteCARD
Reading error while read- -- Try a different CuvetteCARD
ing CuvetteCARD -- Check Reading Unit for Chip- and CuvetteCards
Inserted card may be in service mode
defect. -- Replace PCB mainboard

CuvCARD Checksum error

-- Check for correct CuvetteCARD
Checksum error while
-- Try a different CuvetteCARD
reading CuvetteCARD
Warning: Please remove

ChipCard
-- Remove Card, press Enter key, retry as
Enter: continue
instructed
CuvetteCard inserted in
wrong direction
ChipCard
-- Remove Card, press Enter key retry a different
Enter: continue
Card
CuvetteCard already
devaluated
ChipCard
-- Remove Card, press Enter key, retry a Cuvette-
Enter: continue
CARD
This card is not a Cuvette-
CARD
Remaing.balan.= 500
< keep >
There is a remaining bal- -- Check for existing cuvettes in stock
ance of “n” cuvettes if available remove CuvCARD, press ESC key
still left on the HumaClot
Junior
Remaing.balan.= 500
< keep >
-- Check for existing cuvettes in stock
There is a remaining bal-
ance of “n” cuvettes if not available select < overwrite >,
still left on the HumaClot Press Enter key, continue proceeding
Junior
106
Table 54
Problem /
Possible solution
Display message
Cuvette balance:
100 measurements
While start up procedure of -- Check for further cuvettes and Cuvette-
HumaClot Junior CARDs on stock.
the system indicates that -- Order new cuvettes
100 cuvettes or less
are available.
Warning -- Remove Card, press Enter key,
Wrong cuvette type -- Use matching cuvettes / CuvetteCARD
Wrong cuvette type -- Contact Human service
Warning
-- Remove Card
Invalid customer ID
Wrong distributor ID code
Illegal card!
Please remove it -- Remove Card
Not certified Card or data on -- Contact Human service
Card
Empty/invalid card!
-- Remove Card
Empty or not certified Card or
data on Card
System loads cuvettes Less than 100 cuvettes left on HumaClot
directly from card Junior. System loads new volume automat-
without showing ically from inserted CuvCARD without user
remaining balance communication
Balance fully used!
Only xx reserve measurm.
remain possible!
======================== -- Check for further cuvettes and Cuvette-
*** Please *** CARDs on stock.
*** order new *** -- Order new cuvettes
*** Cuvettes *** -- Proceed by loading new cuvette volume
========================
Cuvette balance on Analyzer
fully used.
No Card!
There is no ChipCARD inserted -- Insert ChipCARD into Card reading unit
in the Card reading unit.
-- Check for further cuvettes and Cuvette-
Balance and reserve fully used
There is no cuvette CARDs on stock.
balance on Analyzer. -- Load new cuvette balance from CuvCARD

Problem / Table 55
Possible solution
Display message
Balance fully used
CARDs on stock.
There is no cuvette balance
-- Load new cuvette balance from CuvCARD
on Analyzer.
CuvCARD devaluated
All cuvettes on CuvCARD
CARDs on stock.
are loaded to Analyzer. No
cuvettes left on CuvCARD.
Only xx measurements remai-
ning.
Warning:
CARDs on stock.
is getting low. Only xx
measurements possible.
Please order new cuvettes
Warning:
CARDs on stock.
Cuvette balance is getting
low.
Only xx reserve measurements
remain possible.
CARDs on stock.
Warning:
-- Load new cuvette balance from CuvCARD
used up.
108
Table 56
Problem /
Possible solution
Display message
ChipCARD Checksum error
-- Check for correct ChipCARD
Checksum error while reading
-- Try a different ChipCARD
ChipCARD
-- Check for correct ChipCARD
ChipCARD read error
-- Try a different ChipCARD
Reading error while reading
-- Check Reading Unit for Chip- and Cuvette-
ChipCARD.
Cards in service mode
Inserted card may be defect.
-- Replace PCB mainboard
ChipCard
-- Remove Card, press Enter key, retry as
Enter: continue
instructed
CuvetteCard inserted in
wrong direction
ChipCard
-- Remove Card, press Enter key retry a
Enter: continue
different Card
CuvetteCard already
devaluated
ChipCard
-- Remove Card, press Enter key, retry a
Enter: continue
CuvetteCARD
This card is not a
CuvetteCARD
Warning: incompatible me-
-- Remove Card, press Enter key
thod
-- Contact distributor
Enter: continue
Incompatible Method CARD
No Card!
There is no CuvetteCARD
-- Insert CuvetteCARD into Card reading unit
inserted in the Card reading
unit.

Problem / Table 57
Possible solution
Display message
-- Reset write protection of SD-Card (switch)
SD-Card is write protected
-- Check for correct SD-Card type/size
Checksum error while reading
-- Check if SD-Card is formatted correctly
ChipCARD
-- Try a different SD-Card
BOOT-Loader V1.00
update on the memory card! -- Remove SD-Card
Remove card.
the folder name must written exactly as
instructed
Couldn´t open ´parimp.txt´
-- Check file structure on SD-Card
format SD-Card with different photo
camera model.
WARNING Line : ´xxxxxx´
ignored -- Press Enter to over jump message and
ENTER= continue proceed
No valid paramter in line xxx -- Check parameters after import procedure
of PARIMP.* file while param- -- Contact Human s service
eter import from SD-Card
-- Check for proper insertion of SD-Card in
reading unit.
-- Remove SD-Card from reading unit and
No SD-Card detected!
retry to insert SD-Card
SD-Card could not be detect-
ed in SD-Card reading unit.
camera model.
No SD-Card found!
reading unit.
SD-Card could not be detect-
-- Remove SD-Card from reading unit and re-
ed in SD-Card reading unit.
try to insert SD-Card
110

reading unit
-- Remove SD-Card from reading unit and re-
try to insert SD-Card
Write error! -- Try a different SD-Card
Error while writing to -- Check folder structure on SD-Card
SD-Card. -- Check folder name
instructed
camera model
Problem /
Display message
reading unit
SD-Card: init error -- Try a different SD-Card
Initialisation Error while -- Check folder structure on SD-Card
writing data to SD-Card. -- Check folder name
instructed
camera model


reading unit
-- Try a different SD-Card
SD-Card: Root directory error!
Error while Access to SD-Card.
instructed
camera model
Blinking P in the right upper -- Reset write protection of SD-Card (switch)
display corner -- Check for correct SD-Card type/size
Write protection of SD-Card -- Check if SD-Card is formatted correctly
detected -- Try a different SD-Card
-- Analyzer will create folder by itself
-- Make sure that there is enough free mem-
No Folder CD12DATA on SD
ory on SD-Card
Card
-- Format SD-Card filesystem as FAT or
FAT32
-- Analyzer will create folder by itself
-- make sure that there is enough free mem-
No file PAREXP.TXT on SD-
ory on SD-Card
Card
-- Format SD-Card filesystem as FAT or
FAT32
112
Table 59
Problem /
Possible solution
Display message
Paramters are not imported instructed
from PARIMP.AUT file -- Check file structure on SD-Card
camera model.
Paramters are not imported instructed
from PARIMP.DEL file -- Check file structure on SD-Card
format SD-Card with different photo cam-
era model.
-- Check installation parameter file on
SD-Card
installed
-- See chapter 3.8
Table 60 Problem /
Possible solution
Display message
Invalid method param.
-- Check method parameters
Method parameters (Applica-
tion parameters) are incorrect
New name cancelled
-- Enter method name again
Method name was
entered incorrectly
Problem /
Possible solution
Table 61 Display message
-- Check setup in UTILITIES / Port B
set to OFF if no Host System available
-- Ignore message if no Host system availa-
ble BUT Patient ID is required.
HOST not connected
-- Check cable connection to Host System
Connection to HOST system
Port B, USB connection to PC
could not detected.
-- Check for correct virtual ComPort driver
on PC
-- Check if HOST/LIS application is properly
working on HOST computer

Problem / Table 62
Possible solution
Display message
-- Check for cuvette inside measuring
channel remove cuvette inside measuring
channel
(Channel) locked
-- Check for foreign parts in bottom of meas-
Error during photometer
uring channel
check.
-- Check for direct ambient light from lamp
or direct sunlight
-- Check for cuvette inside measuring chan-
nel remove cuvette inside measuring chan-
nel
ERR bright calib ch xx
-- Check for foreign parts in bottom of meas-
uring channel
check.
or direct sunlight
-- Check for cuvette inside measuring
channel remove cuvette inside measuring
channel
ERR dark calib ch xx
-- Check for foreign parts in bottom of
measuring channel
check.
or direct sunlight
-- Wait until Analyzer has warmed up prop-
PHOTOMETER CALIBRAT.
erly.
WARM UP 10:27
-- Start process from beginning pay attention
Analyzer not ready for
to
calibration process.
-- See chapter 3.14
Display shows:
-- Check for cuvettes in the measuring channel
-- Remove cuvette from meas. channel
Calibration procedure failed. -- Retry calibration process
114
Table 63
Problem /
Possible solution
transmitted message
E -- Check conversion parameters
Raw value [sec] [mE] -- Check calibration curve
[mE/Mn] faulty or range -- Check application setup
exceeded -- Contact Human service
-- Check if Analyzer is placed in direct sun-
light
-- Check if Analyzer is placed next to a heat-
ing
-- Check present thermal block temperature
with external thermometer
T
WARN: temp instable
-- Check thermal block connector to PCB
Mainboard
-- Check thermal block sensor (100KΩ at
25°C)
OK
Everything was OK, no errors n/a
or warnings appeared
Table 64 Problem /
Possible solution
transmitted message
< 1234 ** -- - Check conversion parameters
** example threshold value = -- - Check calibration curve
1234 -- - Check application setup
Calculated result < min value -- - Contact Human service
> 1234 ** -- Check conversion parameters
** example threshold value = -- Check calibration curve
1234 -- Check application setup
Calculated result > max value -- Contact Human service
-- Check calibration curve
ERR_lin
-- Check application setup
ERR_no_clot
channel 1 break time
R_DIV0
Division by zero during calcu-
lation


ERR_LOG0
Logarithm of negative value
during calculation
ERR_OVER
Overflow during conversion
into target format.
116

Disassembling / Assembling 117
5 DISASSEMBLING / ASSEMBLING
-- Remove all cables to the Analyzer before opening the instrument.
-- Remove all vials from the thermal block.
-- Make sure that no cuvettes are in the measuring channels.
-- Make sure that no chip card is in the card reader.
-- Make sure that no SD-card is in the card reader.
-- Make sure to work on a clean and soft surface.
-- Collect all screws, clamps and clips in an appropriate container.
-- Make sure that no parts will disappear.
-- Immediately remove all parts which fell into the Analyzer by accident.
-- Leave no loose parts in the Analyzer.
-- During and upon completion of your work make sure that no loose parts are
in the Analyzer.
-- Make sure that no demounted parts will be left over after mounting the
instrument, except exchanged parts.
-- Make sure that exchanged modules have the proper program version. See
the information to the software version in chapter „Software status“.
Assembly
-- Assembling Light Protection Cap
-- Assembling Top- and Bottom
-- Assembling Top Housing w. Thermal Block & Mainboard
-- Assembling LCD-Module
-- Assembling Top Housing
-- Assembling Mainboard w. Thermal Block
118
Assembling Light Protection Cap
Figure 57
Assembling Light Protection Cap
1
3
4
Pos. Description Type Quantity

1 Light protection cap 1
2 Screw M3 x 12 Philips 2
3 Cap holder 1
4 1-Channel Instrument assembled 1

5 2
Figure 58
Assembling Top- and Bottom
1
Housing
7
6

1 Screw 3,0 x 10 Torx 4
2 Rubber feet, black 4
3 Housing bottom 1
4 Housing side part left 1
5 Housing side part right 1
6 Hoursing part red colored 1
7 Housing top (assembled) 1
120
Assembling Top Housing w. Thermal Block & Mainboard
Figure 59
1
Assembling Top Housing w.
2
Thermal Block & Mainboard
5
4
6
7

2 PCB Mainboard w. Thermalblock 1
3 Housing top (mounted) 1
4 Display Cable 1
5 Hex-Screw M3 x 6 1
5.1 Washer S3 1
6 Thermal Switch 1
7 Clamping spring 1

1 2 4
Figure 60
Assembling LCD-Module

2 LCD-Module 1
3 Transparent display foil 1
4 Top Housing (assembled) 1
122

Hardware components 123
6 HARDWARE COMPONENTS
HumaClot Junior consists of Printed circuit Boards, Keyboard Overlays, Housing,
Cables, Thermal Block and small parts, each component will be described in de-
tails in the following pages.
6.1 Printed circuit boards

-- PCB Mainboard
-- LCD Module
-- Power supply
PCB Mainboard
Controls most of the hardware and the software functions
Figure 61
PCB Mainboard
Hardware functions:
-- Display control
-- Keyboard control
-- Control of measuring channel
1. Mixer-motor control
2. Light-source and –sensor control
Serial and USB port control

-- ChipCARD and SD-Card control
Software functions:
-- Control of the analytical measurement range
-- Method selection
-- Calculation of the results
-- Output of the final results
124
HumaClot Junior serial number could only be programmed at manufacturer’s

site, so if the serial number was known Human GmbH will program the serial
number during production, if not it will be programmed using the PCB spare
part serial number and because of that the programmed serial number will start
with the letter “S” (Refers to spare part), then the instruments printout will show
the PCB´s serial number instead of the original serial number of the Analyzer.
LCD-Module
Figure 62
LCD-Module
Hardware function:
-- For a better communication with the HumaClot Junior everything will be
displayed on the LCD-module.
Power supply
Figure 63
Power Suppply
Hardware function:
-- Transform the mains voltage into small voltage

Technical data: Table 65

Input voltage: Ue 100-240VAC 50/60Hz
Output voltage: Ua 12VDC 1,0A
6.2 Membrane Keyboard
Figure 64
Hardware Function:
-- Responsible for the data Entries by pressing the keys to communicate with
the Analyzer.
Keyboard matrix
Figure 65
Figure 66
126
6.3 Housings
-- Housing unit
-- Display foil
-- Rubber feet
Housing Unit
Consist of top and bottom housing, left and right side housing
Figure 67
Housing Unit
Pos. Description Quantity / unit

1 Including bottom housing 1 pcs
2 Including top housing 1 pcs
3 Including Housing side part right 1 pcs
4 Including Housing side part left 1 pcs
5 Including transparent display foil 1 pcs
6 Including Hosing part red colored 1 pcs
7 Including lettering 1 set
8 Including rubber feet, black 4 pcs
9 Screws (for assembling top- and bottom housing) 1 set
Comment: Refer to:

Without PCB MAINBOARD
Without LCD-MODULE
Without MEMBRANE KEYBOARD
Without RATING PLATE
Without CABLE FOR DISPLAY
Without THERMAL BLOCK

Display foil
Figure 68
Dispaly foil
Specifications:
Indicator: Comment: Refer to:

Basic colour: transparent
Quantity: 10 pcs. per set
Rubber feet
Figure 69
Indicator: Comment: Refer to:

Basic colour: black
Quantity: 20 pcs. per set
128
6.4 CABLES
-- Cable for display
-- Data cable Mini-DIN / SUB-D
-- Data cable DPU-414
-- Data Cable for BC-Reader
-- USB Cable
-- Barcode Reader
-- RS232 TEST Plug
Cable for display

This cable is responsible for electrical connection between the PCB mainboard
and the LCD module.
Figure 70
Cable for display
Data cable Mini-DIN / SUB-D

This Cable is responsible for connecting the HumaClot Junior with the Comput-
er, by connecting the serial port (SUB-D) with the computer and the Cable data
(MiniDIN) with the Anlyzer.
Figure 71
Data Cable Mini-DIN/SUB-D

Pin Assignment for Data cable Mini-DIN / SUB-D:
Figure 72
Data Cable DPU-414

This cable is responsible for connecting the HumaClot Junior with (DPU-414)
printer, by connecting the serial port with the printer and the other end with
the Analyzer.
Figure 73
Data Cable DPU-414
Pin Assignment for Data Cable DPU-414:
Figure 74
130
Data cable for BC-Reader

This Cable is responsible for connecting the HumaClot Junior with barcode
reader, by connecting the serial port with the barcode reader and the other end
with the Analyzer.
Figure 75
Data cable for BC-reader
Pin Assignment for Data cable for BC-Reader:
Figure 76
USB CABLE
This cable is responsible for connecting the HumaClot Junior with the computer,
by connecting one of USB Ports with computer and the other with the Analyzer.

Figure 77
USB Cable
Barcode Reader
This cable is used to import the patient IDs into the HumaClot Junior.
Figure 78
RS232 Test plug

A loopback connector with RS232 and an LED indicator to check the serial port
of the Analyzer.
Figure 79
RS232 Test plug
132
Pin Assignment for Data cable for RS232 Test plug:
Figure 80
Y-CABLE
This cable is responsible for connecting the HumaClot Junior with the DPU-414
printer and the barcode reader at the same time.
Figure 81
Y- Cable

Pin Assignment for Data cable for Y-Cable:
Figure 82
6.5 Thermal Block

Temperature controlled unit consist of heating foil and temperature sensor, to
pre warm the cuvettes and pick up test cuvettes that are filled with sample and
reagent.
Figure 83
Thermal Block
134
Specifications:
Comment:
Incl. heating foil
Incl. Temperature sensor
Incl. Temperature switch
Incl. Clamp
Without light protection caps and holders 115-93-150-00
Colour: Cuvette type:

Black FL45-cuvette
6.6 Small Parts

Thermal Switch
A safety switch for an over temperature protection for the thermal block.
Figure 84
Thermal Switch
Mixer and Stirrer Motor

This motor is located inside the measuring cuvette for mixing and stirrer the
sample and the reagent.
Figure 85
Mixer and Stirrer Motor

Light Protection cap

The light protection cap covers the measuring cell and reduces the risk of user
error by adapting the pipette tips inside the black hole.
Figure 86
Light Protection Cap
Specifications:
Indicator: Cuvette type:

Basic colour: Black
Marking / Indicator: n/a
Hole diameter for pipette: 5,0 mm
Suitable for: yellow Eppendorf Tip
Contents:
Pos. Description Quantity / unit

1 Light protection cap, black 1 pcs
2 Cap holder, black 1 pcs
3 Screw 3x12mm Philips, black 1 pcs
Set of Fuses
These spare fuses are used for an electrical protection for the Analyzer and the
power supply.
Figure 87
Set of Fuses
136
6.7 Block wiring diagram
Figure 88

6.8 Connectors Pinout

PCB main board connectors pinout
Figure 89
Top view of PCB Mainboard
connector pin out
Figure 90
PCB MAIN BOARD FOR HumaClot
Junior - bottom view
LCD-Module connector pinout
Figure 91
Top view of the LCD-Module
138
Figure 92
Bottom view of the LCD-Module
6.9 Pin Assignment

Pin Assignment for PCB Mainboard
Table 66
SD-Card connector (ST 1) Pin Assignment
1:CS
2:Data IN
3:GND
4:VCC
Figure 93 5:CLK
6:GND
7:Data Out
8:n.c.
9:n.c.
DET
WP
COM
RS232-Connector (ST 3) Serial Port A Pin Assignment
1:+5VDC
2:GND
Figure 94 3:CTS
4:n.c.
5:TxD
6:RTS
7:n.c.
8:RXD

USB (ST 4) USB-B

Figure 95
1:+5V
2: D-
3: D+
4:GND
LCD-Module (ST5) Pin Assignment

1:GND 11:D1 Figure 96
2:+5VDC 12:D2
3:Contrast 13:D3
4:A0 14:D4
5:E1 15:D5
6: E2 16:D6
7:n.c. 17:D7
8:n.c. 18:/RES
9: R/W 19: LED A
10: D0 20: LED K
Keyboard Connector 1 (ST6) Pin Assignment

1:Z1
2:Z2 Figure 97
3:Z3
4:Z4
5:Z5
6:S1
7:S2
8:S3
(see keyboard matrix chapter 4)
Keyboard Connector 2 (ST7) Pin Assignment

1:Z4 Figure 98
2:Z5
3:S4
4:Z3
(See keyboard matrix in chapter 4)
140
Pin Assignment
C1:VCC
C2:RES
C3:SCL
C4:n.c.
CARD reader (ST8) C5:GND
C6:VPP
C7:SDA
C8:n.c.
S1:SW
S2:SW
Power Supply Connector (ST9) Pin Assignment

Figure 99
+ 12VDC
– GND
Thermal Switch Connector (ST10) Pin Assignment

Figure 100
1:12VDC
2:to heater
Not used (STR11) Pin Assignment
Thermalblock Connector(STR12) Pin Assignment
Figure 101
1: Heat +
2: Heat +
3: Heat -
4: Heat -
5: NTC +
6: NTC -

Pin Assignment LCD-MODULE FOR HumaClot Junior
LCD-Module Connector (X1) Pin Assignment Table 67

1:GND 11:D1
2:+ 5VDC 12:D2
Figure 102
3:Contrast 13:D3
4:A0 14:D4
5:E1 15:D5
6:E2 16:D6
7:n.c. 17:D7
8:n.c. 18:RES
9:R/W 19:LED A
10:D0 20:LED K
142

APPENDiX 143
7 APPENDIX
7.1 Software Structure

Figure 103
144

HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 • 65205 Wiesbaden • Germany
Tel.: +49 6122/9988 0 • Fax: +49 6122/9988 100
eMail: human@human.de • www.human.de

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HumaClot Junior

Cat No. 18680/02

SERVICE UND SUPPORT

5 DISASSEMBLING / ASSEMBLING 117

6 HARDWARE COMPONENTS 123

1.2 User Warranty

1.3 Intended Use of the Instrument

1.4 General Safety Warnings

1.5 Disposal Management Concept

HumaClot Junior | Service manual

1.6 Biohazard Warning

1.7 Instrument Disinfection

-- For all the operations described in this manual, wear latex

HumaClot Junior | Service manual

2.1 SERVICE MODE

2.1.1 ACCESS TO THE SERVICE MENU

! Note: Each keystroke is confirmed by a beep tone.

It is not possible to correct the service code.

To re-enter the service code the Analyzer should be turned off

2.1.2 SERVICE FUNCTIONS

HumaClot Junior | Service manual

For detailed procedure please see chapter 3.14.

CH1 Measuring channel 1

LEDx = 0 Actual target value for LED

! Note: The values can be diffe-

The acceptable ranges:

HumaClot Junior | Service manual

The acceptable ranges:

Minimum Maximum Unit Table 2

-- Press Enter key when < heating-test > is displayed.

Heating 0%  Actual target value heating power [%]

The Acceptable ranges:

HumaClot Junior | Service manual

-- Press Enter key when < Port A-test > is displayed

-- The functions TxD => RxD will be checked (loop back).

The key codes:

Key Display Key Display Table 5

-- Press Enter key when < Port B-test > is displayed.

The key codes:

HumaClot Junior | Service manual

-- Press Enter key when < keyboard-test > is displayed.

The key codes:

Key Display Key Display Table 7

! Note: A valid CuvetteCARD

erased and devaluated by this

HumaClot Junior | Service manual

-- Press Enter key when < ext 5V-supply-test > is displayed:

-- When this message below is displayed:

Pressing 1 key switches 5VDC on (indicator LED ON)

-- To leave this function press ESC key.

Nebenlicht test (ambient light test)

LED =0  Target value for LED

Example about the previous test:

-- Press Enter key when < calib. temperature > is displayed:

HumaClot Junior actual adjustment temperatures which

HumaClot Junior | Service manual

-- Press Enter key when < default parameters > is displayed:

-- This message below will be displayed:

-- Press Enter key.

-- Press ESC key when this below message is displayed:

-- Check / calibrate thermal block temperature.

-- This below message will be displayed:

-- To leave this function press ESC key.

5 DISASSEMBLING / ASSEMBLING 117

6 HARDWARE COMPONENTS 123