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1 Supplementary material
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3 Inula helenium and Grindelia squarrosa as a source of compounds with anti-
4 inflammatory activity in human neutrophils and cultured human respiratory epithelium
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6 Barbara Michalak1*, Wojciech Gierlikowski2, Katarzyna Bekier1, Krystyna Skalicka-
7 Woźniak3, Monika E. Czerwińska1, Anna K. Kiss1*
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9 Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of
10 Warsaw, Banacha 1, 02-097 Warsaw, Poland,
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11 Department of Internal Medicine and Endocrinology, Medical University of Warsaw,
12 Banacha 1a, 02-097 Warsaw, Poland
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13 Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin,
14 Chodzki 1, 20-093 Lublin, Poland
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17 Corresponding authors:
18 Barbara Michalak, Department of Pharmacognosy and Molecular Basis of Phytotherapy,
19 Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland, phone +48-22-572-09-
20 85, barbara.gierlikowska@wum.edu.pl
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22 Anna Karolina Kiss, Department of Pharmacognosy and Molecular Basis of Phytotherapy,
23 Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland, phone +48-22-572-09-
24 85, akiss@wum.edu.pl
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39 Figure S1. HPLC-DAD chromatograms recorded at 254 nm (black line) and 325 nm (green
40 line) and MS data for alkamides and triterpenes glycosides in A. pyrethrum, A. ptarmica and
41 H. annuus extracts.
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68 Figure S2. The influence of extract treatment on neutrophils membrane integrity [%] of
69 propidium iodide positive cells PI (+). Data were expressed as the mean ± SEM; at least three
70 independent experiments were conducted, and they were assayed in triplicate. Experiments
71 were performed using cells from different donors.

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75 Figure S3. The influence of extract treatment on A549 cells membrane integrity [%] of
76 propidium iodide positive cells IP (+). Data were expressed as the mean ± SEM; at least three
77 independent experiments were conducted, and they were assayed in triplicate.

Death cells [%]

NST 9.3 ± 2.2
ST (+LPS) 16.8 ± 1.2
ST(+LPS) + I.helenium extract
25 µg/mL 12.1 ± 1.8
50 µg/mL 21.1 ± 1.2
100 µg/mL 22.8 ± 2.3
ST(+LPS) + G.squarrosa extract
25 µg/mL 11.6 ± 7.4
50 µg/mL 11.6 ± 1.0
100 µg/mL 11.2 ± 1.9
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