Issue No.

1
Title:

Document No: Page No.

SOP/QC/023 Page 1 of 3
SOP for Disposal of Media Containing Growth

Effective Date Revision Date

SOP for Disposal of Media Containing Growth

Prepared by： X X
Department Name Date

Checked by： Y Y
Department Name Date

Approved by： Z Z
Department Name Date

1. Purpose
 Issue No.
1
Title:

Document No: Page No.

SOP/QC/023 Page 2 of 3
SOP for Disposal of Media Containing Growth

Effective Date Revision Date

This procedure defines operating procedure to discard media containing growth in plates and
tubes obtained during analysis.

2. Scope

This procedure is applicable to applicable to discard media containing growth in plates and
tubes obtained during analysis. in

3. Validity

This SOP is valid only until next revision date and if it bears control seal.

4. Responsibility

It will be the responsibility of Tech.Assistant ( Microbiologist)to follow the procedure.

Quality assurance manager is responsible for SOP compliance.

5. Material and Equipment

Glassware, 0.1 % Teepol solution

6. Procedure:
6.1. Wear sterile gloves, goggles and mask before starting the work.
6.2. Place the discarded plates & tubes with media containing microbial growth in S.S.bowl.
6.3. Place the S.S.bowl in vertical autoclave for sterilization as per S.O.P.
6.4. After completion of sterilization cycle, dilute the liquidified media approximately ten
times with hot water & drain it in washing area.
6.5. Wash them with tap water.
6.6. Then wash the glassware with 0.1 % Teepol solution (prepared from Purified Water) .
6.7. Remove the marker ink present on glassware using methanol.
6.8. Rinse the glassware three times using Purified water.
6.9. Mop the outer surface of the glassware with a clean cotton cloth.
 Issue No.
1
Title:

Document No: Page No.

SOP/QC/023 Page 3 of 3
SOP for Disposal of Media Containing Growth

Effective Date Revision Date

6.10. Place them into the oven for drying.

6.11. Use dry glassware.
6.12. Clean the glass wares using hot nitric acid as per S.O.P, whenever require.
6.13. Disposal of Media filled Ampoule/Vial
6.13.1. Wear gloves, goggles & mask before starting the work.
6.13.2. Arrange the media filled ampoules/vials along with tray in the sterilizer.
6.13.3. Close the lid of sterilizer properly and run the sterilization cycle as per SOP (more than
one cycle may require in case if sterilizer capacity does not sufficient to accommodate all
ampoules/vials)
6.13.4. After completion of sterilization cycle allow the article to cool down to normal
temperature & take out the media ampoule/vial out to the wash basin platform.
6.13.5. Remove the seal, closure / break the ampoule/vial & collect the media in a suitable size .
6.13.6. Dilute the collected media in a ratio approx 1:10 with tap water in a between and drain it
in basin. Continue the practice during disposal of entire quantity.
6.13.7. Collect the empty / broken ampoule/vial and seal in a separate waste bin in a plastic bag.
6.13.8. Tie the open end of plastic bag properly and transfer it to scrap disposal area.
6.13.9. Record the details of Sterilization cycle