Cell Respiration Lab

https://www.youtube.com/watch?v=r9o_VdToClE

Aerobic cellular respiration is the release of energy from organic compounds by metabolic chemical
oxidation in the mitochondria within each cell. Cellular respiration involves a series of enzyme‐mediated
reactions. The equation below shows the complete oxidation of glucose. Oxygen is required for this
energy‐releasing process to occur

C6H12O6 + 6O2 6CO2 + 6H2O +686 kilocalories of energy

mole of oxidized glucose
1. Using the equation above, how could you measure the rate of cell respiration?
2.
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3. How is the rate of respiration measured in this

lab?____________________________________________________________________

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4. Draw and label a respirometer.

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5. Explain how the respirometer is used to measure oxygen consumption? Include specifics

regarding the set‐up.

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6. Why is KOH necessary to accurately measure oxygen consumption?

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7. Describe the contents of the three respirometers used in this lab? Which one is the
control?
Respirometer #1‐

Respirometer #2‐

Repsirometer #3‐

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8. Why does water move into the respirometer?

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9. Imagine that you have been given 25 germinating pea seeds that have been placed in

boiling water for 5 minutes. You place these seeds in a respirometer and collect data.

Predict the rate of oxygen consumption for these seed and explain your reasons.

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10. Imagine that you are asked to measure the rate of respiration for a 25 g reptile and a
25 g mammal at 10oC. Predict how the results would compare and justify your
prediction. (*Hint: reptiles are ectothermic and mammals are endothermic)
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11. Iff respiration in a small mammal
m (mo
ouse) and larrge mammall (elephant) were studieed at
10 degrees Ce
elsius, which
h organism would
w have a higher respiration ratee and why?
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12. In
nterpretation of Graphicc Analysis

nating and non‐germinating

a. Calculate and compare the reacction rates of the germin
corn at each tempe
erature (slop
pe). What ca n you concluude from this informatio
on?

b. What can
n you conclud
de from these results?

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