T.C.

ÇUKUROVA UNIVERSIY
SCIENCE AND LITERATURE FACULTY
CHEMISTRY DEPARTMENT.
BIOCHEMISTRY LABORATORY I

Analysis of protein in a blood plasma/serum samples

Daniel Alejandro MALAVER RANGEL
2019144315
Purpouse of the experiment: Analyzing a sample of blood in order to determny the
quantity of protein that it contains.

Introduction
A blood protein test, commonly referred to as a serum protein test, serves as a fundamental
diagnostic tool, offering vital insights into the delicate equilibrium of proteins within the
bloodstream. These proteins, predominantly albumin and globulin, play pivotal roles in
maintaining overall health, and their quantification is essential for a comprehensive
understanding of physiological well-being. In conjunction with this analysis, the biuret
reagent, a copper-containing solution, is employed to enhance precision, adding a valuable
layer to the protein measurement process.
Albumin, a major component of blood serum, contributes significantly to the maintenance of
oncotic pressure and serves as a vital transporter for hormones and drugs. Deviations in
albumin levels may signal underlying conditions such as liver or kidney disease, malnutrition,
or inflammatory disorders.
Complementing albumin, globulins encompass a diverse group of proteins, including
antibodies crucial for immune function and other transport proteins. Anomalies in globulin
levels can point to immune system disorders or other underlying health issues.
The collaborative examination of these proteins, along with the application of the biuret
reagent, provides healthcare professionals with a nuanced perspective on an individual's
health status. This comprehensive approach facilitates the diagnosis of various medical
conditions, ranging from chronic inflammation and infections to autoimmune disorders or
nutritional defic
 Calculations
Most of the calculations were made in excel
 Conclusion
The experiment can be divided into several main parts, we can summarize the whole
procurement: the separation of the albumin from the serum, the preparation and calibration of
the model samples with a fixed solution of proteins. And finally, the analytical process of
measuring the U.V. absorption of each of the tubes of the solution.
As a reference point it is important to remark how a normal blood sample of a healthy person
might behave. Most of the time a value of protein in the blood system of 8d/dl or6d/dl is
accepted, which 4-3mg/dl of it is compoused of albumin. Most of the cases albumin forms
part of the %0% of the protein quantity of blood. Comparing these values with the values
obtained in the experiment we can see that the quantity of protein in the blood is quite close to
the one that a healthy human being might present, however, a 2g/dl of albumin in the blood
system it is not something that is normal in nature. ds
This facilitates the analysis due to the easiness of deduction of such abnormal value, since the
main protein value is pretty much accurate but the fact that albumin is alarmingly low might
show a systematic error at the moment to extract albumin from the serum.
At whole, the experience was concluded successfully.

References and articles

 C-W Yu, L-I Juan, M-H Wu, C-J Shen, J-Y Wu, C-C Lee, Systematic review and meta-
analysis of the diagnostic accuracy of procalcitonin, C-reactive protein and white blood
cell count for suspected acute appendicitis, British Journal of Surgery, Volume 100,
Issue 3, February 2013, Pages 322–329, https://doi.org/10.1002/bjs.9008
 G Basha, F Penninckx, P Yap, Influence of blood components and faeces on the in
vitro cancericidal activity of povidone–iodine, British Journal of Surgery, Volume 85,
Issue 4, April 1998, Pages 534–537, https://doi.org/10.1046/j.1365-2168.1998.00631.x
 Zheng Zhang, Min Zhang, Shengfu Chen, Thomas A. Horbett, Buddy D. Ratner,
Shaoyi Jiang, Blood compatibility of surfaces with superlow protein
adsorption,BiomaterialsVolume 29, Issue 32,2008