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Culture Documents
مايكرو عملي فصل اول
مايكرو عملي فصل اول
Autoclave
✦ Use / used for sterilizing culture media, glassware, and other materials that
are not spoiled by moist heat .
✦principle /Moist heat & pressure.
Incubator
Use / used for providing microorganism with optimum temperature for living .
Principle / electrical heat .
Hot air oven
Use / used for sterilizing glassware & other materials thar are spoiled by moist heat .
Principle / Dry heat
Inoculating loop
Use / used to heat or melt sample and reagents , also used to water heating in
beaker .
Principle / indirect heat
Hot plate
Use /used for observing microscopic items such as cells, crystals and cell organelles .
Refrigerator
Use / used for sterilizing loop, plating out culture and transferring culture .
Spirit lamp
Use/The same as burner but lamb is portable abs used as fuel) produces free smock
flam .)
Lab balance
Sterelization methods
✦ Physical method
✦mechanical method
✦chemical method
By filteration technique
Used to sterilize sera, solutions of sugar , vaccines, toxins and antibiotics .
Culture media /Solutions containing all of nutriebts and physical parameters necessary for microbial growth .
Q/Classification of media
A/Based on composition
•Natural media consist of natural materials such as meat and peptone .
•Synthetic media consist of chemical materials
•Semisynthetic media consist synthetic materials in addition to natural materials .
•Basic media /contains peptone, salt, water, source of carbon and source of nitrogen .
Ex .Nutrient broth, Nutrient agar
•Enrchied media /basic media that are supplmented with vitamin, body fluid, amino acid
and protein .
Ex .Blood agar and chocolate agar
Types of hemolysis of bacteria
Differential media / used for diffrentiatin between types of bacteria such as diffrentiation between lactose
_fermenting&non _lactose fermenting bacteria .
اذا اكو تخمر يكون الوسط لونه وردي واذا مااتخمر الالكتوز يكون الوسط
Colorless .
•Selectiv e media /used for growth of gram negative bacteria only such as macconky agar
T. B. C. S /selectiv e media , used for growth of v ibrio cholera
•S_S agar/differential ➡️differs between salmonella and shigella .
✦✦Differential media
•Macconky agar
•S_S agar
•Blood agar
Mannitol salt agar
Indicator media /contains ph indicator that change the colorr of media according to ph.)Mannitol salt agar)