How does FACS measure the cell size?

a. Using SSC values

b. Using fluorescence values
c. Using FSC values
d. Using the FSC/SSC ratio

What is the interrogation point in FACS?

a. Where the cells intercept the laser beam trajectory
b. Where the cells are loaded into the fluidics system
c. Where the cells are sorted, depending on the software settings
d. Where the cells are mixed with the flow buffer

Why is it so important to set a good drop delay in FACS?

a. It allows the system to decide when to apply the electric charge to the droplets
b. It allows the system to decide when to define the interrogation point
c. It allows the system to prevent contamination
d. It allows the system to maintain a stable pressure

What does the droplet break-off point refer to in FACS?

a. It’s the point on the stream where it begins to be partitioned into droplets
b. It’s the point where the laser intercepts the stream
c. It’s the point where the cells are mixed with the flow buffer
d. It’s the point on the stream where droplets are sorted

Match the following descriptions with the appropriate type of biosensor described:

G. Measures the change in potential A. Colorimetric biosensor

difference due to mechanical stress.
B. Potentiometric biosensor
F. Measures the change in temperature
following an enzymatic reaction. C. Optical biosensor

A. Measures the intensity of color change. D. Amperometric biosensor

B. Measures the change in voltage using E. Photometric biosensor

pH meters.
F. Calorimetric biosensor
D. Measures the change in current.
G. Piezoelectric biosensors
This technique involves the use of a micropipette to form a seal with the cell membrane allowing
one to measure either voltage or current across it.
a. Pinch clamp
b. None of the above
c. Patch clamp
d. Tight clamp

___________ biosensors relate the concentration of the analyte to the resulting current produced.
a. Voltametric
b. Amperometric
c. Potentiometric
d. Piezoelectric

This equation allows the determination of the cell potential under non-standard conditions (i.e., not
at STP).
a. Nernst equation
b. Huxley equation
c. Hodgkin equation
d. Nerst equation

Which of the following are uses of FACS?

a. All of the above
b. Determine cell cycle phase
c. Sort the different white blood cells
d. Determine the presence/absence of specific enzymes

Wheelchairs and digital thermometers are classified as a:

a. Class I medical device
b. Class II medical device
c. Class IV medical device
d. Class III medical device

This technique was used to study the action potential in giant squid axons.
a. Voltage clamp
b. Current clamp
c. Resistance clamp
d. Pinch clamp
What is the flow buffer in FACS made of?
a. Distilled water
b. Saline solution
c. Ethanol
d. LB media

These are instruments that are used in the prevention, monitoring, or diagnosis of illnesses and
diseases.
a. diagnostic device
b. medical instrument
c. biomedical device
d. biosensor

In the Philippines, this is the organization responsible for approving new medical devices.

FDA

DOH

DTI

DOF

This characteristic refers to the ability of the biosensor to detect even low concentrations of a
specific analyte.
a. Linearity
b. Selectivity
c. Sensitivity
d. Response Time

This is the signal generated when neurons fire and send electrochemical signals across the nervous
system.
a. electric potential
b. membrane potential
c. action potential
d. voltage potential
This type of biosensor utilizes the difference in electrical signals as a result of applied mechanical
stress.
a. Optical
b. Thermoelectric
c. Calorimetric
d. Piezoelectric

What is the main purpose of the flow cell in FACS?

a. To sort the samples
b. To create a uniform stream for the samples in the flow buffer
c. To create a uniform stream of flow buffer
d. To add pressure to the flow

The following materials can serve as bioreceptors for biosensors, EXCEPT:

a. Antibodies
b. Enzymes
c. Microorganisms
d. Bioplastic

This classification of medical device features general controls only.

a. Class IV
b. Class I
c. Class II
d. Class III

Biosensors are comprised of the following general parts, EXCEPT:

a. Transducer
b. Bioreceptor
c. None of the given choices
d. Reaction Layer

The resting membrane potential of neurons is:

a. -70 mV
b. -65 mV
c. -60 mV
d. -75 mV
What is the function of the closed-loop nozzle in FACS?
a. Ensures a good fluidic system preparation
b. Creates a uniform stream to sort cells
c. Ensures a good fluidic system start-up and shutdown procedure
d. Maintains the pH of the flow buffer

What is the number of events per second in FACS?

a. The amount of sample in microliters per second that is loaded into the fluidics system
b. The number of cells per second that are being loaded into the fluidics system
c. The number of times that the laser beam intercepts a single cell
d. The number of cells that is going directly to waste

___________ biosensors relate the change in charge distribution using ion-selective probes.
a. Amperometric
b. Voltametric
c. Curremetric
d. Potentiometric

Why is pressure so important in FACS?

a. To move both the sample and the flow buffer through the system
b. To avoid contaminants passing through the column
c. To keep the solutions stable in the tanks
d. To avoid mixing the sample with the solutions

All of the following are Class III medical devices, EXCEPT:

coronary stents

pacemakers

orthopedic implants

catheters

Which of these components is not part of the FACS optics system?

a. The collection lenses
b. The lazer beam
c. The nozzle
 d. The dichroic mirrors

Which is a Class IIA medical device?

a. an elastic bandage
b. a breast implant
c. a ventilator
d. a blood transfusion tube

DNA hybridization detection is considered which type of biosensor:

piezoelectric

potentiometric

calorimetric

amperometric

A new moisture meter is tested using test weights of varying masses and is performed on the same
day, in the same lab, with the same conditions. Which of the following is most likely the result we
can get from this test?
a. Selectivity
b. Sensitivity
c. Response Time
d. Accuracy

___________ biosensors relate a change in color to the concentration of a given analyte.

a. Colorimetric
b. Optical
c. Calorimetric
d. Photometric

This refers to the time taken by the biosensor to change its output from an initial state to a final
settled value, usually 95% of the response.
a. Response Time
b. Sensing Time
 c. Reaction Time
d. Characteristic Time

These devices are comprised of specific biological elements and a transducer to detect specific
analytes.
a. biotransducer
b. medical device
c. biosensor
d. biomedical device

These more advanced medical devices require strict regulation and approval prior to application.
a. Class I
b. Class II
c. Class III
d. Class IV

Changes in the oscillation frequency of cantilever are used to detect changes in the mass onto them.
This is true for which type of sensor?
a. piezoelectric
b. impedimetric
c. amperometric
d. optical

The currenctly accepted model for transmission of information across nerve membranes is the:
a. Huxley Model
b. Hodgkin-Huxley Model
c. Hodgkin-Hershey Model
d. None of the above

The ___________ produces the electric response through a physical, chemical, or biological change,
which is then measured by the transducer.

analyte

sensor

bioreceptor
 signal

This refers to the ability of biosensors to only detect the specific analyte of interest, minimizing
chemical interference.
a. Response Time
b. Selectivity
c. Sensitivity
d. Linearity

This parameter of biosensor design is the interval between upper and lower levels.
a. Range
b. Resolution
c. Stability
d. Precision

This characteristic of biosensors states that the higher the concentration of the substrate, the higher
the detected signal.
a. Response Time
b. Linearity
c. Sensitivity
d. Selectivity

__________ biosensors relate the change in intensity of photon output to the concentration of an
analyte.
a. Colorimetric
b. None of the choices
c. Photometric
d. Optical

To design biomedical devices, one must understand both __________ and __________ aspects of the
device.

biological; electrical

biological; mechanical

mechanical; electrical
 None of the choices

This biosensor relates the measured intensity of light with the concentration of the analyte.
a. Electrochemical
b. Optical
c. Calorimetric
d. Piezoelectric

A biosensor may be classified according to their:

a. both A and B
b. bioreceptor used
c. neither A nor B
d. transducer used

These medical devices often require the implementation of special controls as they present a
moderate risk.
a. Class I
b. Class II
c. Class IV
d. Class III