CEUSUE

3 SPICe S i t e
S'SpHCa sile
lo ohat is SplieeaSe me 2
-hat
3 laigeRNA Prolein Complex.
h e Spltee osome s
Catalyse Ihe Penoval in foo om muatean Poe- mRNA.

Cuhu i-4 sound

Pmmail, it is found nmue leus of enk auo He ezl,
6son Exon 2

U4/uG SnRNP In 1947, uonk byJhe shanp and Roberts labs mevealed
US sn.RNNP

Laruar fopmakon lhat emun hihen Onganisms spu'+0n Pneenin

and 5'9pli 3Ae
US eleaya Seuenal dshinet SeqmeutS alm Jhe DNA mol t u t .

U2 The mtrono ar ex er se d omn h Peeun) on m RNAS

GExen 2 3 m a Proeem
shanp mamed "spliein

iga ion of ozon. Phillip shanp and Rie kand

J. Roberds wera. awande d +h
1013 Nobet PTe in- physro loa
Ex ens
axon h diseouiny o nbon and Pucny Poca.
EtG.URE (A):- 9teps in h
intom
PGeina o# nu elaan
Pne-mpNA
OPe Tht retuo Pep o Splieso Sa man lne mpRNA.
Ma 3 pitaoiemas -

Thy aesaunt o ht
Th nemoval 9.5/.
ionmid rm
a
nr
i , U2, Uu, Us, Uc SnRNPs. spliaslem
Minn uceo SomaS->-hi at SnRNPs
Thuy ar to nm cowtisn t h e
6val 3.51. Eton on
mUi, U12, v4atae , U5, and ae.
5
UCatac Sn 2NPs.
Mechanus mS
rne -m RNA
Eised inmon
in lauak shapu.
nmr Spln Ccew uth
Cempu Gausd in a
lans mbe
Ths
spucae temei,
P eea te ne enaino
w hch anembls nuclcopoin 5
E20T .
z
on Pre- mRNA,
md
U5, wweh a r amooated SnRNAS U v2 and
Mawu m.RNAA-
+he U4fu6
non m 2NP
h Smal Complo
mbonuelean Proeino,
POn faesrn c. amd
Th sPuny
reae or is
miiakd oy OReFERENCES
2'- hjdo th
i'ea bond ot 5 P th braneh Lite ha
nutlusphi ie attaek
adde no tYna on he Brochem'stny »nd Molacan bisloan *
-

day and the bnane 3Pea c

Th5 Auans Sina ha 3'>5' phespho Db
Plams tsaeand sdrton
h
ae rii ne tohn enon-
.
buekauan, wlhdm Gmuissan- and
tonm a 2' sTnton bcun
- epii bo nd, Russell L. Jonun).
9runahrha. ariat Inaami diate
Phosphodi 3true huu and
Assomby 0t
spuerrz is Com PlRd uiih *a eend and
neuanrrr he ' erzon 4h
3plieeo Lemal Sn. RNPs
3' hydul arup h nu elsophi be astaeh k.Noga, Y Mu DA.
Pomor.an krumm, C. kambaeh
5"ezen on h by t h s. walke and ,T.ImJakue,
2' sPUe e hie h
Phos pho dieeslen bo nd at ih A,
kuglstatton)
h u enon amd -th
aniat. nelusning J h intron- Phasphoy! "Tnawnfen Paaeton im Pne- mRNA
.Maiano A. Gareia Ltan to...
-

spluein
w'kireda Saganmoy hah).