KWAME NKRUMAH UNIVERSITYY

SCHOOL OF NATURAL SCIENCES

DEPARTMENT OF LIFE SCIENCES

NAME : JOHN MANDONA

KNU : 120230252

COURSE CODE : BIO 5511

COURSE NAME : ADVANCED MICROBIOLOGY

LECTURER : MS. HANYUMA

QUESTION : “A reorganization of the phylogenetic system of classification of

organisms to include the Domain (a taxonomic category above the Kingdom level) has been
proposed by scientists in recent years.
Discuss the composition of this classification system and explain why it is more inclusive
than the Robert Whittaker five-kingdom classification system. “
 QUESTION ONE

Gene recombination, also known as genetic recombination, plays a crucial role in genetic
research and has contributed significantly to our understanding of biology and medicine.
Here are some of the key contributions and applications of gene recombination in research:

Creation of Genetically Modified Organisms (GMOs), The utilization of gene

recombination methods, exemplified by recombinant DNA technology, has empowered
scientists to insert particular genes into the genetic material of organisms. This has resulted in
the development of genetically modified crops that exhibit improved characteristics,
including heightened resilience to pests and enhanced nutritional value.

Recombinant DNA technology has revolutionized agriculture by enabling the creation of

genetically modified organisms (GMOs), particularly crops with enhanced traits. Through
this technology, scientists can selectively introduce specific genes into the genomes of plants,
leading to the development of crops with improved resistance to pests, diseases, and
environmental stressors. For instance, the incorporation of genes encoding insecticidal
proteins, such as those from Bacillus thuringiensis (Bt), has allowed the creation of
genetically modified crops with inherent resistance to certain pests. Additionally, researchers
have utilized gene recombination to enhance the nutritional content of crops, exemplified by
Golden Rice, which produces beta-carotene to address vitamin A deficiency in populations
reliant on rice.

The benefits of genetically modified crops extend beyond pest resistance and nutritional
enhancement. Some crops are engineered for herbicide tolerance, enabling more effective
weed control without harming the main crop. This not only simplifies agricultural practices
but also contributes to increased crop yield. The ability to tailor crops for specific traits
through gene recombination holds great promise for addressing global food security
challenges by providing farmers with tools to produce more resilient and productive crops.

Production of Recombinant Proteins, Gene recombination is widely used to produce

recombinant proteins for various purposes. By inserting a gene coding for a particular protein
into a host organism, researchers can leverage the host's cellular machinery to produce large
quantities of the desired protein. This has applications in medicine, where recombinant
proteins are used as therapeutic agents, and in industry for the production of enzymes and
other valuable proteins.
Gene Therapy, Recombinant DNA technology is a foundation for gene therapy, a field
aimed at treating or preventing genetic disorders by introducing, removing, or modifying
specific genes. Gene recombination allows for the precise manipulation of genes to correct
genetic defects, and it holds great potential for the treatment of various diseases.

Functional Genomics, Gene recombination is instrumental in functional genomics studies,

where researchers investigate the functions of genes and their interactions within biological
systems. Techniques such as RNA interference (RNAi) use gene recombination to selectively
silence or knock down specific genes, helping researchers understand the role of those genes
in various cellular processes.

Creation of Knockout and Transgenic Animals, Gene recombination has been used to
create knockout mice and other transgenic animals. Knockout animals have specific genes
inactivated, allowing researchers to study the effects of gene loss on development and
physiology. Transgenic animals carry foreign genes, providing insights into the functions of
those genes and their impact on the organism.

Vaccine Development, Recombinant DNA technology has played a crucial role in the
development of vaccines. For example, the expression of viral proteins in yeast or bacteria
using gene recombination allows for the production of vaccine antigens, leading to safer and
more effective vaccines.

Study of Evolution and Phylogenetics, Comparative genomics, facilitated by gene

recombination studies, allows researchers to trace the evolutionary relationships between
different species. By analysing gene sequences, scientists can infer evolutionary patterns and
understand the genetic basis of diversity among organisms.

Drug Development, Understanding the genetic basis of diseases through gene recombination
has implications for drug development. Targeted therapies that address specific genetic
factors associated with diseases can be developed based on the knowledge gained from
studying gene function and expression patterns.

In summary, gene recombination has revolutionized molecular biology, enabling researchers

to manipulate and understand genetic material with unprecedented precision. This has led to
advancements in various fields, from agriculture and medicine to basic biological research
and the development of new therapeutic approaches.
 QUESTION TWO

Biosensors, Biosensors are analytical devices that integrate a biological component (such as
enzymes, antibodies, or whole cells) with a physicochemical detector to detect the presence
of specific substances or changes in biological conditions. These devices are designed to
provide rapid and accurate measurements of various parameters, such as the concentration of
specific molecules (e.g., glucose, pathogens, or pesticides) or changes in biological activities.

Merits:

Precision in Detection, Biosensors offer high specificity and sensitivity in detecting various
biological and chemical components. This precision is crucial for identifying specific
pathogens, pesticides, or nutrients in agriculture. Biosensors can detect pathogens and
diseases at early stages, enabling timely intervention and preventing the spread of diseases in
crops

Real-Time Monitoring, Biosensors enable real-time monitoring of environmental conditions

and the presence of contaminants. This capability is valuable for timely decision-making in
agricultural practices, helping farmers respond promptly to changes in the field. Continuous
monitoring provided by biosensors helps in making informed decisions in real-time,
optimizing the use of fertilizers and other inputs

Cost-Effectiveness, Compared to traditional laboratory methods, biosensors can be more

cost-effective. They often require fewer resources and provide rapid results, reducing the
overall cost of monitoring and diagnostics in agriculture.

Demerits:

Limited Multiplexing, Some biosensors may have limitations in simultaneously detecting

multiple analytes. This can be a drawback when dealing with complex agricultural systems
where multiple factors need monitoring simultaneously.

Maintenance and Calibration, Biosensors require regular maintenance and calibration to

ensure accurate and reliable results. Improper maintenance can lead to sensor drift or
malfunction, affecting the quality of data obtained. Effective use of biosensors requires
technical knowledge, and some farmers may find it challenging to operate and interpret the
data generated by these devices.
Interference and Specificity Challenges, Environmental factors and interference from other
compounds in the sample can sometimes impact the specificity of biosensors. Achieving high
specificity can be challenging in certain agricultural settings.

Bioaccumulation, Bioaccumulation refers to the gradual build-up of substances, typically

chemicals or pollutants, in the tissues of living organisms over time. This process occurs as
organisms absorb these substances from their surrounding environment, often through food or
water intake. Bioaccumulation is particularly relevant in the context of environmental science
and ecology, where it can lead to an increase in the concentration of certain substances in
higher trophic levels of a food chain, potentially causing ecological and health concerns.

Merits

Phytoremediation: Bioaccumulation in plants can be harnessed for phytoremediation, where

plants accumulate and detoxify pollutants from the soil. This eco-friendly approach helps in
soil cleanup and environmental restoration. Bioremediation: Certain plants and
microorganisms have the ability to accumulate and detoxify pollutants from the soil, water,
and air, contributing to the clean-up of contaminated agricultural sites.

Nutrient Cycling: Bioaccumulation plays a role in nutrient cycling, with plants accumulating
essential nutrients from the soil. This is particularly important for maintaining soil fertility
and supporting crop growth. Plants that accumulate nutrients can release them back into the
soil upon decomposition, promoting nutrient cycling and reducing the need for synthetic
fertilizers.

Demerits

Toxicity Concerns, While bioaccumulation can be beneficial for certain nutrients, it can also
lead to the accumulation of toxic elements in plants. This is a concern, especially when crops
are grown in contaminated soils, as it may pose risks to human and animal health. The
bioaccumulation process can lead to the concentration of toxic substances in certain plant
tissues, posing risks to both animals and humans if these plants are consumed.

Limited Plant Species, Not all plant species exhibit efficient bioaccumulation, and the success
of this process depends on the plant's ability to absorb and tolerate specific contaminants.
This limits the range of crops that can be effectively used for bioaccumulation.
Bioaccumulation is often a slow process, and the effectiveness of remediation may take
years, limiting its immediate impact in addressing urgent environmental concerns
Biomineralization, Bioaccumulation refers to the gradual build-up of substances, typically
chemicals or pollutants, in the tissues of living organisms over time. This process occurs as
organisms absorb these substances from their surrounding environment, often through food or
water intake. Bioaccumulation is particularly relevant in the context of environmental science
and ecology, where it can lead to an increase in the concentration of certain substances in
higher trophic levels of a food chain, potentially causing ecological and health concerns.

Merits:

Soil Improvement, Biomineralization processes, such as microbial-induced mineral

precipitation, can contribute to soil improvement by enhancing soil structure and nutrient
availability. Some microorganisms are capable of mineralizing organic matter into inorganic
forms, contributing to the improvement of soil structure and fertility.

Carbon Sequestration, Certain biomineralization processes can facilitate carbon sequestration

in the soil, helping mitigate the effects of climate change by trapping carbon dioxide.
Biomineralization processes can contribute to carbon sequestration, helping mitigate climate
change by storing carbon in the soil.

Demerits

Variable Efficience, The efficiency of biomineralization processes can vary depending on

environmental conditions, microbial activity, and the specific minerals involved. Achieving
consistent and predictable outcomes can be challenging. Biomineralization processes are
highly dependent on environmental factors such as temperature and moisture, which may
limit their effectiveness in certain regions or under specific climatic conditions.

Time-Consuming, Biomineralization processes may take time to yield noticeable effects on

soil properties. In situations requiring rapid improvements, this time factor can be a
limitation. The range of minerals that can be produced through biomineralization is limited,
and it may not address all soil nutrient deficiencies.

In summary, while biosensors, bioaccumulation, and biomineralization offer several

advantages for agricultural technology, they also come with certain limitations and
challenges. The suitability of these approaches depends on the specific agricultural context
and the goals of the farming practices involved
 REFERENCES
Schaechter, M. (Ed.). (2003). Desk Encyclopedia of Microbiology. ISBN: 0126213615.
Retrieved from Coe Library Stacks - Level 4. (Call Number: QR9 .D47 2004).

Singleton, P., & Sainsbury, D. (2006). Dictionary of Microbiology and Molecular Biology.
ISBN: 0470035455. Retrieved from Coe Library Stacks - Level 4. (Call Number: QR9.S56
2001).

Robertstad, G. W., McClung, L. S., & Maki, L. R., 30(6), 557-565. (1968). The American
Biology Teacher, Published by University of California Press.

Achtman, M., & Wagner, M. (2008). Microbial diversity and the genetic nature of
microbial species. Nature Reviews Microbiology, 6(6), 431-440.

Ackermann, H. W. (2011). Bacteriophage taxonomy. Microbiology Australia.