Scribd Logo
Upload

Welcome to Scribd!

  • Chapter 4

    Uploaded by

    Iman
    10 views1 page
    The document discusses the effect of temperature on enzyme activity. It shows that enzyme activity is inactive at 0°C and reaches its maximum at 40°C. Above 40°C, enzyme activity decreases and stops as the enzyme becomes denatured at high temperatures. Another factor that can similarly affect enzyme activity is pH. Some enzymes require cofactors like metal ions or coenzymes to facilitate their activity. If a competitive inhibitor is added, it will bind to the enzyme's active site preventing substrate binding and product formation, slowing the reaction rate.

    Original Description:

    Original Title

    CHAPTER 4

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The document discusses the effect of temperature on enzyme activity. It shows that enzyme activity is inactive at 0°C and reaches its maximum at 40°C. Above 40°C, enzyme activity decreases and stops as the enzyme becomes denatured at high temperatures. Another factor that can similarly affect enzyme activity is pH. Some enzymes require cofactors like metal ions or coenzymes to facilitate their activity. If a competitive inhibitor is added, it will bind to the enzyme's active site preventing substrate binding and product formation, slowing the reaction rate.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    10 views1 page

    Chapter 4

    Uploaded by

    Iman
    The document discusses the effect of temperature on enzyme activity. It shows that enzyme activity is inactive at 0°C and reaches its maximum at 40°C. Above 40°C, enzyme activity decreases and stops as the enzyme becomes denatured at high temperatures. Another factor that can similarly affect enzyme activity is pH. Some enzymes require cofactors like metal ions or coenzymes to facilitate their activity. If a competitive inhibitor is added, it will bind to the enzyme's active site preventing substrate binding and product formation, slowing the reaction rate.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    BIOCURE PROGRAMME

    TOPIC 4: BIOCATALYSIS
    BIOLOGY UNIT, KOLEJ MATRIKULASI MELAKA

    1 The effect of temperature on enzyme activity is shown in FIGURE 1.

    Temperature (°C)

    FIGURE 1

    a) Describe the activity of enzyme at 0°C and 40°C.


    [2 marks]
    At 0°C : activity of enzyme is inactive
    At 40°C : activity of enzyme is at maximum

    b) Explain the activity of enzyme if the temperature exceeds 40°C.


    [2 marks]
    Activity of enzyme will be decreased and will stop because enzyme is denatured at
    high temperature.

    c) State another ONE factor that will have similar effect as shown by the temperature
    above.
    [1 mark]
    pH value

    d) Name TWO cofactors that are required by some enzymes to facilitate their activities.
    [2 marks]
    - Metal ion activators
    - coenzymes

    e) Explain what happen if a competitive inhibitor is added to an enzymatic reaction.


    [3 marks]
    The competitive inhibitor will bind to an active site of the enzyme. This will prevent
    substrate from bind to active site. Enzyme-subtrate complex cannot form. Thus,
    product do not form. Hence, rate of reaction will slow down.

    You might also like