Type Of Test Sample 1: Sample 2: Sample 3: Sample 4: RESULT

Observation
Observation Observation Observation

Although sodium When I add a The burner is The egg and The copper (II) ion
Biuret Test hydroxide can copper sulfate used to heat test gelatin and the protein's
produce protein solution to the tubes a and b, dispersion's peptide link
denaturation egg white and and after heating, albumin white combine to
when used to gelatin dispersion, both test tubes part turns purple produce a purple
fresh egg white, it takes on a turned purple. when heated with coordination
little changed in purplish color. a copper sulphate complex in an
color once I and sodium alkaline solution.
added it to the hydroxide The name of this
egg albumin and solution. compound is
gelatin biuret.
dispersion.
Nothing changed Nothing changed Furthermore, After being The
Xanthoproteic in color when I in color when I concentrated heated in the xanthoproteic
Test added also added nitric acid was burner, both test acid is what
concentrated concentrated added to the tubes' contents causes the yellow
nitric acid to the nitric acid to the gelatin dispersion produced a precipitate. It is
egg albumin. egg albumin. solution, but no yellow created when
color change precipitate. some proteins'
happened. amino acids, like
tyrosine and
tryptophan, are
nitrated.
Nothing Nothing changed It took on a bluish An excellent This test is
Ninhydrin Test happened when I when I added color. outcome and the primarily used to
added the Ninhydrin presence of an identify proteins
Ninhydrin solution to the amino acid in the and amino acids
solution to the gelatin sample are with free NH2
egg albumin. dispersion. indicated by the groups by forming
formation of a an intensely blue-
bluish violet colored amino
complex in the derivative when
tube. they react with
ninhydrin.
The phenol group There is no Positive outcome:
Millon’s Test of tyrosine ions in Characteristic A precipitate with
the solution form changes. a red or pink
a red precipitate. color. Negative
effect The
absence of
colored
precipitate.
Type of Test Sample 1: Observation Sample 2: Sample 3:
RESULT
Observation Observation

Solubility Test Desi Ghee Vegetable Ghee Refined Oil

Oil or fat are present

Water Immiscible Immiscible Immiscible in all samples.

Oil or fat are present

Alcohol Dissolves Dissolves Dissolves in all samples.

Chloroform Miscible Miscible Miscible

Translucent Spot Test Translucent Translucent Translucent

Acrolein Test Pungent irritating Pungent irritating Pungent irritating

odour is odour is odour is
present/produced present/produced present/produced

Baudouin Test

Concentrated Pure Desi Ghee: Vanaspati Ghee:

Hydrochloric Acid and Does not contain Contains sesame oil
Furfural Solution sesame oil

Huble Test

Cotton Seed Oil: Linseed Oil:

Chloroform and Huble Saturated Unsaturated
Agent
SCREENSHOTS