1 WAY TO 100 – BIOCHEMISTRY

CHAPTER – 3 PAST QUESTIONS

7-The amino acid which contains an indole group is:

a. Cystine
b. Tryptophan
c. Histidine
d. Arginine

11- What is the net charge on the peptide Gly Arg-Lys-Asp at pH 1?

A +2
B +1
C -2
D+3

12 - Amino acids with hydrophobic side chains are zwitterions when they are
a. in alkaline solutions only
b. in any solution
C.at physiological pH
d. in acidic solutions only

17 - Basic amino acids are……………. (positive, negative) at pH 7 and acidic R group amino
acids are➖➖➖➖➖(positive, negative) at pH7.
a. positive; positive
b. negative; negative
c. negative; positive
d. positive; negative

19- The amino acid in which the R group has a net positive charge at pH 7.0 is____
a. Glutamine.
b. Serine.
c. Lysine.
d. Glycine.

23 -Based upon its side chain, how is the amino acid Ser classified?
A.non-polar
B.polar
C.negatively charged
D.positively charged
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28- Glycine is not a stereoisomer because

a. it has no chiral carbon.
b. it does not form enantiomers.
C. it does not exist in two non superimposable mirror-image forms. all of the above
D.all of the above

38- The true statement about solutions of amino acids at physiological pH is:
a.Some amino acids contain only positive charge.
B.All amino acids contain negatively charged side chains.
C.All amino acids contain positively charged side chains.
D.All amino acids contain both positive and negative charges.

1.Which amino acid would have the greatest negative charge at pH = 7.0?
Select one:
a) Histidine
b) Glutamic Acid
c) Serine
d) Tyrosine

2.Based upon its side chain, how is the amino acid Serclassified?
Select one:
a) Polar
b) positively charged
c) negatively charged
d) non-polar

4.Which amino acid has the three-letter symbol asn?

a) asparagine
b) alanine
c) aspartic acid
d) arginine

5.Which of the following amino acids has a side chain that can make hydrogen bonds to
other molecules?
a) Gly
b) Ala
c) Ser
d) Leu
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20.What is the charge on the tetrapeptide: lys-lys-his-glu at pH 7?

Select one:
a) -1
b) +1
c) +2
d) 0

22.The side chain of serine could interact with the side chain of_______ using_____
Select one:
a) His; hydrogen bond
b) Gln; hydrophobic interaction
c) Val; hydrophobic interaction
d) Asn; electrostatic interaction

23.Which group consists only of amino acids with polar side chains?
Select one:
a) serine, threonine, and cysteine
b) serine, threonine, and leucine
c) serine, threonine, and isoleucine
d) serine, threonine, and valine
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31. Amino acid have the zwitterion structure:

a) when the molecule carries a net positive charge
b) when the molecule carries a net negative charge
c) at very high ph
d) at their isoelectic point (given the symbol pI)

35.which of the following has an aromatic side chain

a) alanine
b)tyrosine
c) valine
d) arginine

39.which of the following amino acid has an amide bond inits R group
a)Asn
b) His
c) val
d)ser

40.which of the following amino acid has a thiol group in its r group
a) cys
b) met
c) ser
d) asn

4 - All proteins contain the

a. Only a few amino acids
B. 300 Amino acids occurring in nature.
C. Different amino acids
d. Same 20 amino acids
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7-The amino acid which contains an indole group is:

a. Cystine
b. Tryptophan
c. Histidine
d. Arginine

12 - Amino acids with hydrophobic side chains are zwitterions when they are
a. in alkaline solutions only
b. in any solution
C.at physiological pH
d. in acidic solutions only

17 - Basic amino acids are................ (positive, negative) at pH 7 and acidic R

group amino acids are (positive, negative) at pH7.
a. positive; positive
b. negative; negative
c. negative; positive
d. positive; negative

19- The amino acid in which the R group has a net positive charge at pH 7.0
is__
a. Glutamine.
b. Serine.
c. Lysine.
d. Glycine.

23 -Based upon its side chain, how is the amino acid Ser classified?
A.non-polar
B.polar
C.negatively charged
D.positively charged
7 WAY TO 100 – BIOCHEMISTRY

2) At a pH above its pKa, the phenolic group of tyrosine is _.

a) protonated and neutral
b) protonated and positively charged
c) deprotonated and neutral
d) deprotonated and negatively charged
e) none of these choices

3) At a pH above its pKa, the -amino group of lysine is _.

a) protonated and neutral
b) protonated and positively charged
c) deprotonated and neutral
d) deprotonated and negatively charged
e) none of these choices

4) At a pH above its pKa, the R-group of Asp is _.

a) protonated and neutral
b) protonated and positively charged
c) deprotonated and neutral
d) deprotonated and negatively charged
e) none of these choices

6) At pH 3, how many charged groups are present in the pentapeptideAla-Asp-

His-Ser-Lys?
a) 1
b) 2
c) 3
d) 4
e) 5
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8) Which of the following amino acids has a positively charged side chain at pH
7.4?
a) Glu
b) Ala
c) Lys
d) Leu
e) His

9) Which of the following amino acids has a thiol group in its side chain?
a) Ser
b) Asn
c) Cys
d) Tyr
e) Met

10) Which of the following amino acids has a nonpolar side chain?
a) Ser
b) Val
c) Lys
d) Asn
e) none of these choices

11) Which of the following amino acids contains a side chain that is polar and
uncharged from pH 1-13?
a) Phe
b) Tyr
c) Asp
d) Gln
e) Ile
9 WAY TO 100 – BIOCHEMISTRY

13) At pH _, the pentapeptideAla-Glu-His-Val-Cys would contain two positively

charged groups and one negatively charged group.
a) 1
b) 3
c) 5
d) 7
e) 9

14) Disulfide bonds form between two residues of which amino acid?
a) Met
b) Ser
c) Asn
d) Thr
e) Cys

15) Based upon its side chain, how is the amino acid Ser classified?
a) non-polar
b) polar
c) positively charged
d) negatively charged e) none of these choices

16) At physiological pH, all amino acids have at least _ charged groups.
a) 0
b) 1
c) 2
d) 3
e) cannot be determined
10 WAY TO 100 – BIOCHEMISTRY

19) What is the net charge of the penta-peptide Ala-Cys-Ser-Glu-Asn at pH 7?

a) -2
b) -3
c) 0
d) +1
e) none of these choices

5)Which amino acid would have the greatest negative charge at pH = 7.0?
A. Serine
B. Glutamic Acid
C. Histidine
D. Isoleucine

22)Which one of the following amino acids may be considered a hydrophobic

amino acid at physiological pH of 7.4?
A. Isoleucine
B. Arginine
C. Aspartic acid
D. Threonine
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Q1. A given amino acid has the following pKa values: a-COOH= 2.15, a-NH3+= 8.95, and side
chain (RH+)= 10.53. then its PI Value is :
a) 7.21
b) 5.55
c) 6.34
d) 9.74

Q2. Which of the following is a pentapeptide?

a) Carnosine.
b) Glutathione.
c) Oxytocin.
d) Enkephalin.

Q3. What is the net charge on the peptide phe-Glu-Lys-Met at pH12?

a) Zero
b) +1
c) -2
d) +2

Q4. How many equivalents of OH- you need to completely titrate glutamic acid?
a) 1
b) 2
c) 3
d) 4

Q5. Which of the following amino acids has hydrophobic (non-polar) side chain?
a) Leu
b) Glu
c) Cys
d) Ser

Q6. During titration with a strong base glutamine can exist in :

a) One ionic form
b) Tow ionic forms
c)Three ionic forms
d) Four ionic forms
12 WAY TO 100 – BIOCHEMISTRY

Q7. The peptide bond, characterized by rigid motion in proteins, is formed between:
a) The alpha-carboxyl group of one amino acid and the alpha-amino group of the next one.
b) The alpha-carbon of one amino acid and the alpha-amino nitrogen of the next one.
c) The alpha carbon and the alpha-amino nitrogen of the same amino acid.
d) The alpha-carbon and the alpha-carboxyl carbon of the same amino acid.

Q8. How many inflection points are there in the titration curve of valine?
a) 1
b) 2
c) 3
d) 4

Q9. Which of the following amino acids has the largest side chain group?
a) Valine
b) Phenylalanine
c) Tyrosine
d)Tryptophan ring

Q10. Which of the following amino acid pairs are non-polar?

a) Val and Asp
b) Try and Arg
c) Leu and Alanine
d) Leu and Lys

Q11.Which of the following is an example of a sulfur-containing amino acid?

a) Serine
b)Methionine
c) Tryptophane
d) Aspartic acid

Q12. Which of the following is a cyclic peptide?

a) Carnosine
b) Glutathione
c) Oxytocin
d) Enkephalin
13 WAY TO 100 – BIOCHEMISTRY

Q13. The isoelectric point of an amino acid is the point at which the molecule: -
a) Is polar with overall zero charge
b) Is polar with overall negative charge
c) Is polar with an overall positive charge
d) Is nonpolar

Q15. What is the net charge on the peptide: phe-Glu-Lys-Met at pH1?

a) Zero
b) +1
c) -1
d) +2

Q16. Which statement is NOT CORRECT about the peptide bond?

a) The peptide bond has partial double-bound character-
b) The peptide bond is longer than the typical carbon-nitrogen bond
c) Rotation is restricted about the peptide bond
d) The carbonyl oxygen and the amide hydrogen are most often in a trans configuration with
respect to one another

Q17. For a given amino acid the pKa of the alpha COOH group:
a) Is higher than the pKa of the alpha,-NH3+ group
b) Is lower than the pKa of the alpha, -NH3+ group
c) Equals to the pKa of the alpha,, -NH3+ group
d) Equals to the PI of base

Q18. How many inflection points are there in the titration curve of histidine
a) 1
b) 2
c) 3
d) 4
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Q19. Which of the following amino acids has a -CONH2 group in its side chain?
a) Glutamine
b) Glutamic acid
c) Tyrosine
d) Lysine

Q20. Which of the following amino acids is not chiral?

a) Proline
b) Glycine
c) Alanine
d) Lysine

Q21. Which of the following amino acids is a precursor of Thyroxine?

a) Tryptophan
b) Threonine
c) Tyrosine
d) Lysine

Q23 what is the net charge on the peptide: Trp-His-Asp-Ala at pH 12?

a) Zero
b) +1
c) -1
d) -2

Q24.How many inflection points are there in the titration curve of alanine?
a) 1
b) 2
c) 3
d) 4
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Q25. Which of the following amino acids is a precursor of Thyroxine?

a) Tryptophan
b) Threonine
c) Tyrosine
d) Lysine

Q26. Which of the following amino acid pairs are polar?

a) Val and Asp
b) Arg and Glu
c) Leu and Met
d) ile and Lys

Q27. During titration with a strong base asparagine can exist in:
a) One ionic form
b) Tow ionic forms
c) Three ionic forms
d) Four ionic forms

Q28. Which of the following amino acids has hydrophobic (non-polar) side chain?
a)Val
b) Glu
c) Cys
d) Ser

Q29. Which of the following amino acids is uncommon?

a) Phenylalnine
b) Tyrosine
c) Valine
d) Thyroxine

Q30.Which of the following is a hydrophobic substance?

a) Alanine
b) Ethanol
c) Sucrose
d) Benzene