Antibiotic resistance of Salmonella from marketed Penaeus monodon shrimps

The development of antibiotic resistant strains and multiple antibiotic resistance in

bacteria are showed major importance since which may lead to resistance transfer to food
borne pathogenic bacteria and reduced efficacy of antibiotic treatment for human.
Therefore, the present study was carried out to assess the occurrence of resistance to
antimicrobials in Salmonella that has been isolated from marketed Penaeus monodon
shrimps in North Western Province, Sri Lanka. The study design used to fulfil the above
objective was the cross sectional survey. Isolation of salmonellae was done according to
ISO 6579 (2002) standard guidelines; microbiology of food and animal feeding stuffs-
horizontal method for the detection of Salmonella spp. The antibiotic susceptibility
testing of all isolates was carried out using the Disk Diffusion Test according to the
National Committee of Clinical Laboratory Standards (NCCLS, 2000). The slide
agglutination test was used in serotyping for Salmonella based on the Kauffman-White
scheme.
All 23 strains isolated from shrimps were tested for antimicrobial resistance. 100 % of all
the 23 isolates of Salmonella were observed susceptible for nalidixic acid, ampicillin,
gentamycin, sulphamethoxazole/ trimethoprim, chloramphenicol, ciprofloxacin,
trimethoprim and tetracycline. One strain was susceptible to all 14 antimicrobials tested.
Twenty-two strains were detected for resistance and all these strains were resistant to two
antimicrobials (erythromycin and sulphonamides) of which one was resistant to three
antimicrobials (erythromycin, sulphonamides and amoxycillin). Out of 22 resistant
serotypes S. Newport showed the highest resistance (50.00 %) followed by S.
Weltevreden, E15, B4, F-67 each (9.09 %). The lowest was observed for S. Mbandaka,
E10 and C7 each (4.55 %). The percentage antimicrobial resistant isolates from culture
shrimps was higher than from capture shrimps without being statistically significant
(p>0.05). In overall out of 14 different kinds of antibiotics tested the isolates of
Salmonella showed resistance to 2 kinds of antibiotics namely erythromycin and
sulphonamides in a higher percentage (95.65%). The control of infectious diseases is
seriously threatened by the steady increase in the number of micro-organisms that are
resistant to antimicrobial agents. Hence, these findings are timely important to assure the
safety and quality of shrimps to control the food borne Salmonella infections.
 Antibiotic resistance of Salmonella from marketed Penaeus monodon shrimps

J.K.H.Ubeyratnea, G. Hildebrandtb, J. Kleerb, R. Khattiyac

a
Veterinary Research Institute, Gannoruwa, Peradeniya, Sri Lanka
b
Institute for Food Hygiene, Faculty of Veterinary Medicine, Freie Universität Berlin,
Germany
c
Faculty of Veterinary Medicine, Chiang Mai University, Thailand
Dr. J.K.H.Ubeyratne
Veterinary Investigation Centre
Veterinary Research Institute
Gannoruwa
Peradeniya
14.03.2008

Prof. R. Sivakanesan
Dept.of Biochemistry
Faculty of Medicine
Peradeniya

Abstract for oral presentation

Herewith I am sending the abstract of the research topic Antibiotic resistance of

Salmonella from marketed Penaeus monodon shrimps for the oral presentation at the
Sri Lanka Veterinary Association 60th Annual Scientific Sessions 2008.

Thanking you

………………………………
Dr. J.K.H. Ubeyratne