Home Work #4 - Biotechnology: Principles and

Processes Contact Number: 9667591930 / 8527521718

1. Which gene transfer technique involves the use of a 8. Ti-plasmid is used for making transgenic plants. It is
fatty bubble to carry a gene into a somatic cell? obtained from
1. electroporation 1. Azotobacter
2. liposome transfer 2. Agrobacterium
3. microinjection 3. Rhizobium in leguminous root
4. particle bombardment 4. Yeast

2. Which of the following is normally not used as a 9. Production of human protein in bacterial genetic
method of introduction of engineering is possible because
foreign DNA into an animal cell? 1. Human chromosome replicate bacterial cell
1. Microinjection 2. Liposomes 2. Mechanism of gene regulation is identical in humans
3. Biolistics 4. Transfection and bacteria
3. Bacterial cell can undertake RNA splicing
4. Genetic code is universal
3. Human insulin is being commercially produced from
a transgenic species of 10. For transformation, micro-particles coated with
1. Rhizobium DNA to be bombarded with gene gun are made up of:
2. Saccharomyces 1. Silver or Platinum 2. Platinum or Zinc
3. Mycobacterium 3. Silicon or Platinum 4. Gold or Tungsten
4. Escherichia

4. Transgenic plants are the ones: 11. A method used only for transforming animal cells is:
Grown in an artificial medium after hybridization in 1. Biolistics 2. Microinjection
1.
the field. 3. Use of virus 4. Agrobacterium mediation
Produced by a somatic embryo in an artificial
2.
medium.
Generated by introducing foreign DNA into a cell 12. Bacillus thuringiensis forms protein crystals that
3.
and generating a plant from that cell. contain insecticidal protein. This protein:
Produced after protoplast fusion in an artificial does not kill the carrier bacterium, which is itself
4. 1.
medium. resistant to this toxin.
binds with epithelial cells of midgut of the insect pest
2.
ultimately killing it.
5. Some of the characteristics of BT cotton are 3. is coded by several genes including the gene cry.
1. Medium yield, long fibre and resistance to beetle pests is activated by the acid pH of the foregut of the insect
2. High yield and production of toxic protein crystals 4. pest.
which kill dipterans pests
3. High yield and resistance to bollworms
4. Long fibre and resistance to aphids 13. The two polypeptides of human insulin are linked
together by
6. The first human hormone produced by recombinant 1. Hydrogen bonds.
DNA technology is 2. Phosphodiester bond.
1. Insulin 3. Covalent bond.
2. Estrogen 4. Disulphide bridges.
3. Thyroxin
4. Progesterone

7. In Bt cotton, the Bt toxin present in plant tissue as

pro-toxin is converted into active toxin due to:
1. Acidic pH of the insect gut
2. Action of gut micro-organisms
3. Presence of conversion factors in insect gut
4. Alkaline pH of the insect gut

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 Home Work #4 - Biotechnology: Principles and
Processes Contact Number: 9667591930 / 8527521718

14. If one needs to derive a cDNA of human insulin,

which cells should be taken up for extraction?
Any cell of the human body as the DNA is the same
1.
in all cells
2. Any nucleated cell of the human body
Beta cells of islets of Langerhans of the human
3.
pancreas
4. WBCs

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