Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur

B. Pharmacy IV Semester
Subject: Pharmacology I
List of Practical

1. Introduction to experimental pharmacology.

2. To Study the Commonly used instruments in experimental pharmacology.
3. To Study the common laboratory animals.
4. To Study the Maintenance of laboratory animals as per CPCSEA guidelines.
5. To Study Common laboratory techniques: Blood withdrawal, serum and plasma
separation techniques.
6. To Study Common laboratory techniques: anaesthetics and euthanasia used for animal
studies.
7. To Study the different routes of drugs administration in mice/rats.
8. To Study the effect of hepatic microsomal enzyme inducers on the phenobarbitone
sleeping time in mice.
9. To Study the effect of drugs on ciliary motility of frog oesophagus.
10. To Study the effect of drugs on rabbit eye.
11. To Study the effects of skeletal muscle relaxants using rota-rod apparatus.
12. To Study the effect of drugs on locomotor activity using actophotometer.
13. To Study the anticonvulsant effect of drugs by MES method.
14. To Study the anticonvulsant effect of drugs by PTZ method.
15. To Study the stereotype and anti-catatonic activity of drugs on rats/mice.
16. To Study the anxiolytic activity of drugs using rats/mice.
17. To Study the local anesthetics by different methods.

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 Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur
B. Pharmacy V Semester
Subject: Pharmacology II
List of Practical

1. Introduction to the in-vitro pharmacology.

2. To prepare 500ml of physiological salt solutions.

3. To study the effect of drugs on isolated frog heart.
4. To study the effect of drugs on blood pressure and heart rate of dog.
5. To study the diuretic activity of drugs using rats/mice.
6. To Study the DRC of acetylcholine using frog rectus abdominis muscle.
7. To study the effect of physostigmine on DRC of acetylcholine using frog rectus abdominis
muscle.
8. To study the effect of atropine on DRC of acetylcholine using frog rat ileum.
9. To perform the bioassay of histamine using guinea pig ileum by matching method.
10. To perform the bioassay of oxytocin using rat uterine horn by interpolation method.
11. To perform the bioassay of serotonin using rat fundus strip by three-point bioassay.
12. To perform the bioassay of acetylcholine using rat ileum/colon by four-point bioassay.
13. Determination of PA2 value of prazosin using rat anococcygeal muscle (by Schilds plot
method).
14. Determination of PD2 value using guinea pig ileum.
15. To Study the effect of spasmogens and spasmolytics using rabbit jejunum.
16. To Study the anti-inflammatory activity of drugs using carrageenan induced paw-
edema method.
17. To Study the analgesic activity of drug using central and peripheral methods.
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Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur

B. Pharmacy VI Semester
Subject: Pharmacology III
List of Practical

1. Dose calculation in pharmacological experiments.

2. To Study the antiallergic activity by mast cell stabilization assay.
3. To Study the anti-ulcer activity of a drug using pylorus ligand (SHAY) rat model.
4. To Study the anti-ulcer activity of a drug using NSAIDS induced ulcer model.
5. To Study the effect of drugs on gastrointestinal motility.
6. To Study the Effect of agonist and antagonists on guinea pig ileum.
7. Estimation of serum biochemical parameters by using semi- autoanalyzer.
8. To Study the effect of saline purgative on frog intestine.
9. To Study the Insulin hypoglycaemic effect in rabbit.
10. To Study the Test for pyrogens (rabbit method).
11. Determination of acute oral toxicity (LD50) of a drug from a given data.
12. Determination of acute skin irritation / corrosion of a test substance.
13. Determination of acute eye irritation / corrosion of a test substance.
14. Calculation of pharmacokinetic parameters from a given data.
15. To Study the Biostatistics methods in experimental pharmacology (student’s t test)
16. To Study the Biostatistics methods in experimental pharmacology (ANOVA)
17. To Study the Biostatistics methods in experimental pharmacology (Chi square test)
18. To Study the Biostatistics methods in experimental pharmacology (Wilcoxon Signed
Rank test)
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