Four Weeks International Online Certificate Course on

“BIOANALYTICAL TECHNIQUES (Part I) – Microscopy,

Chromatography and Centrifugation (Phase - I)”
(for Faculties, Research Scholars and Students)
Proudly Offered By

DEPARTMENT OF MICROBIOLOGY, SACRED HEART COLLEGE

(AUTONOMOUS), TIRUPATTUR, TAMIL NADU, INDIA

RIO DE JANEIRO STATE UNIVERSITY (UERJ), RIO DE JANEIRO, BRAZIL

BIOTECHNOLOGY SOCIETY OF NEPAL

03rd January 2024 to 04th February 2024

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 Four Weeks International Online Certificate Course on
“BIOANALYTICAL TECHNIQUES (Part I) – Microscopy,
Chromatography and Centrifugation (Phase - I)”
Patrons
Rev. Dr. S. Praveen Peter SDB,
Rector and Secretary, Sacred Heart College (Autonomous), Tirupattur
Rev. Dr. D. Maria Antony Raj SDB,
Principal, Sacred Heart College (Autonomous), Tirupattur
Rev. Dr. K. A. Maria Arokiaraj SDB,
Additional Principal, Sacred Heart College (Autonomous), Tirupattur
Rev. Dr. G. Theophil Anand SDB,
Vice Principal (Shift I), Sacred Heart College (Autonomous), Tirupattur
Rev. Dr. S. John Borg SDB,
COE, Sacred Heart College (Autonomous), Tirupattur

Course Conveners
Dr. P. Saranraj,
Head, Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur
Dr. Alexander Machado Cardoso,
Rio de Janeiro State University (UERJ), Rio de Janeiro, Brazil
Dr. Vasudeo Zambare,
Chairperson, Biotechnology Society of Nepal (Indian Chapter), UTM Fellow, Universiti
Teknologi Malaysia.

Course Co-conveners
Dr. M. Manigandan,
Assistant Professor, Department of Microbiology, Sacred Heart College (Autonomous)
Dr. Gláucio Diré Feliciano,
Professor, Rio de Janeiro State University (UERJ), Rio de Janeiro, Brazil
Mr. Nahin Munankarmi,
President, Biotechnology Society of Nepal.

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 COURSE DETAILS
Name of the Course : Bioanalytical Techniques (Part I) – Microscopy,
Chromatography & Centrifugation (Phase I)

Duration : 4 Weeks

Starting Date : 03.01.2024

End Date : 04.02.2024

Registration fees : Rs. 300 for Faculties

Rs. 200 for Students and Research Scholars

Last Date for Registration : 01.01.2024

Link for Registration : https://forms.gle/wx9r4h1CDkZ1ns4eA

Mode of Payment : Payment details will be sent through E.mail

Recorded Videos link : Will be circulated to participants through E.mail

Quiz : 25 MCQs per week

Mode of Evaluation : 25 MCQs for 4 Weeks (Google Form Quiz)

Total Marks : 100 Marks (25 Quiz per week)

Criteria for Certification : 50 % to 59 % – Successfully completed

60 % to 74 % - First Class
75 % and above - Distinction

Course Convener : Dr. P. Saranraj,

Head,
PG and Research Department of Microbiology,
Sacred Heart College (Autonomous),
Tirupattur, Tamil Nadu, India.
Mobile - +91-9994146964 (WhatsApp Number)
E.mail: shcmicrobiology@gmail.com

NOTE
Participants are requested to pay the Registration fees after receiving the Payment
Email from us (Payment details will be sent to Registered E.mail after the completion of
Registration process). For Successful candidates, E-certificate will be provided after filling the
Feedback form and Payment of Prescribed Registration Fees. E-Certificate will be mailed to the
participants during the Last week of February 2024.

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 ONLINE COURSE CONTENTS
CHAPTER
NUMBER TOPICS

WEEK – 1
1 History of Microscopy
2 Parts of Microscope
3 Difference between Simple and Compound Microscope
4 Difference between Light and Electron Microscope
5 Bright Field Microscope
6 Dark Field Microscope
7 Polarizing Microscope
8 Fluorescent Microscope
9 Confocal Microscope
10 Phase Contrast Microscope
11 Differential Interference Contrast (DIC) Microscope
12 Electron Microscope
13 Difference between SEM and TEM
14 Scanned Probe Microscopy – Scanning Tunnelling Microscopy
15 Scanned Probe Microscopy – Atomic Force Microscopy

WEEK – 2
16 An Introduction to Chromatography
17 History of Chromatography
18 Affinity Chromatography
19 Anion Exchange Chromatography
20 Cation Exchange Chromatography
21 Column Chromatography
22 Flash Chromatography
23 Gel Filtration Chromatography
24 Thin Layer Chromatography
25 Paper Chromatography

WEEK – 3
26 Liquid Chromatography
27 High Performance Liquid Chromatography (HPLC)
28 High Performance Thin Layer Chromatography (HPTLC)
29 Gas Chromatography
30 Gas Liquid Chromatography
31 Reverse Phase Chromatography
32 Ion Exchange Chromatography
33 Hydrophobic Interaction Chromatography

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 WEEK – 4
34 An Introduction to Centrifugation
35 History of Centrifugation
36 Analytical Centrifugation
37 Density Gradient Centrifugation
38 Differential Centrifugation
39 Isopycnic Centrifugation
40 Rate Zonal Density Centrifugation
41 Differential Velocity Centrifugation
42 Equilibrium Density Gradient Centrifugation
43 Sucrose Gradient Centrifugation

Note

❖ Some contents may be included/deleted while conducting the course.

❖ Remaining contents will be continued in the Next Course “BIOANALYTICAL
TECHNIQUES (Part II) – Electrophoresis, Chromatography and Spectroscopy”