Type/subtype gene primer Primer Sequence PCR Product size BLAST results

Forward
25 61.26 40.00 2.00 2.00
H1F1 AGCAAAAGCAGGGGAAAATAAAAGC primer
Reverse
24 59.37 45.83 4.00 2.00
HA-5’(H1) H1R1264 CCTACTGCTGTGAACTGTGTATTC 1264 primer
Forward
21 53. 42.86 3.00 2.00
HAFA GGGAGAATGAACTATTACTGG primer
Reverse
21 53.44 33.33 6.00 6.00
HA-3’(H1) HARend AGTAGAAACAAGGGTGTTTTT 979 primer
Influenza A(H1N1)pdm09
Forward
23 56.50 34.78 6.00 4.00
N1F1 AGCAAAAGCAGGAGTTTAAAATG primer
Reverse
24 61.23 45.83 3.00 3.00
NA-5’(N1) N1R1099 CCTATCCAAACACCATTGCCGTAT 1099 primer
Forward
24 60. 45.83 6.00 4.00
N1F401 GGAATGCAGAACCTTCTTCTTGAC primer
Reverse
36 63.14 30.56 6.00 4.00
NA-3’(N1) NARUc ATATGGTCTCGTATTAGTAGAAACAAGGAGTTTTTT 1073 primer
Forward
24 56.27 37.50 7.00 7.00
H3A1F6 AAGCAGGGGATAATTCTATTAACC primer
Reverse
24 60.14 45.83 3.00 1.00
HA-5’(H3) H3A1R1 GTCTATCATTCCCTCCCAACCATT 1127 primer
Forward
24 61.60 41.67 6.00 4.00
H3A1F3 TGCATCACTCCAAATGGAAGCATT primer
Reverse
35 64.32 34.29 6.00 4.00
HA-3’(H3) HARUc ATATCGTCTCGTATTAGTAGAAACAAGGGTGTTTT 863 primer
Influenza A(H3N2)
Forward
29 67.45 48.28 7.00 1.00
NAFUc TATTGGTCTCAGGGAGCAAAAGCAGGAGT primer
Reverse
23 62.28 47.83 7.00 1.00
H9N2 H3N2R1095 TCATTTCCATCATCRAAGGCCCA 1095 primer
Forward
24 60. 45.83 4.00 3.00
N2F387 CATGCGATCCTGACAAGTGTTATC primer
Reverse
36 63.14 30.56 6.00 4.00
NA-3’(N2) NARUc ATATGGTCTCGTATTAGTAGAAACAAGGAGTTTTTT 1082 primer
Forward
20 55.38 45.00 5.00 5.00
FluB‐VicHA‐472F TGCCCTAACATTACCAATGG primer
Reverse
20 58. 55.00 4.00 2.00
Victoria FluB‐VicHA‐668R GAGTCCCCATAGAGCTTTGC 196 primer
Influenza B
Forward
21 60.48 47.62 6.00 2.00
BYf226 ACACCTTCTGCGAAAGCTTCA primer
Reverse
24 57.16 37.50 3.00 0.00
Yamata BYr613 CATAGAGGTTCTTCATTTGGGTTT 388 primer
Forward
22 56.65 40.91 2.00 0.00
THAF2 GCAGGGGAAAATAAAAACAACC primer
Reverse
22 55.85 40.91 2.00 2.00
HA-5’(H1) SPHAR11 TATTTTGGGCACTCTCCTATTG 990 primer
Forward
24 61.31 45.83 4.00 2.00
H1HAF552 TACCCAAACCTGAGCAAGTCCTAT primer
Reverse
35 64.32 34.29 6.00 4.00
HA-3’(H1) HARUc ATATCGTCTCGTATTAGTAGAAACAAGGGTGTTTT 1239 primer
Former seasonal Influenza A(H1N1)*
Forward
24 56.64 33.33 6.00 2.00
H1N1F6 AGCAGGAGATTAAAATGAATCCAA primer
Reverse
21 55.53 42.86 2.00 1.00
NA-5’(N1) NASPR10 CCTTCCTATCCAAACACCATT 1097 primer
Forward
24 61.84 45.83 4.00 2.00
N1F741 ATAATGACCGATGGCCCGAGTAAT primer
Reverse
36 63.14 30.56 6.00 4.00
NA-3’(N1) NARUc ATATGGTCTCGTATTAGTAGAAACAAGGAGTTTTTT 737 primer

-source/influenza/molecular-detention-of-influenza-viruses/protocols_influenza_virus_detection_feb_2021.pdf?sfvrsn=df7d268a_5
Development and evaluation of a conventional RT‐PCR for
differentiating emerging influenza B/Victoria lineage viruses
with hemagglutinin amino acid deletion from B/Yamagata