COURSE INFORMATION (Sesi1 2020/2021)

Course Name : Basic Instrumental Analysis

Course Code : CHM260

Programme : 4 (Diploma in Microbiology AS114)

4 (Diploma in Industrial Chemistry AS115)

5 (Diploma in Science AS120)

Credit Unit : 3.0

Contact Hours : Lectures (3 hours/week), Laboratory practical 3 hours /week) / 120 hours

Course Status : Core

Pre-Requisite : Nil

Lecturer’s Name : Dr. Rafie Draman

Contact No. : 019-9831970

Course Description

This course is an introduction to the theory, application and basic instrumentation for
Chromatography (Gas Chromatography and High Performance Liquid Chromatography) and
Spectroscopy. Molecular absorption spectroscopy discussed includes Ultraviolet, Visible and Infrared.
The types of atomic spectroscopy covered are Flame Atomic Absorption Spectroscopy (AAS) and
Flame Atomic Emission Spectroscopy (AES)

Course Outcomes

1. Relate the theories and formulas to solve spectroscopic and chromatographic problems.
2. Explain the principles of instrumentations, qualitative and quantitative concepts in
spectroscopy and chromatography.
3. Report the functions of spectroscopic and chromatographic components.
4. Perform experiments in spectroscopy and chromatography

Assessment (Covid-19)
Test :
Laboratory Report :
Assignment Presentation :
Final Assessment :
20% - Chapter 1 & 2
10% - Chapter 1, 2, 3 & 4
30% - Chapter 5 & 6
40% - Chapter 3, 4, 5 & 6
References
1. Skoog, D.A., West, D.M., Holler, F.J., Crouch, S.R., Fundamentals of Analytical Chemistry,
8th ed., Thomson Brooks/Cole, 2004.’’ Main Text Book – If you want it tell me – E-BOOK’ -
FREE
2. Skoog, D.A., West, D.M., Holler, Analytical Chemistry: An introduction, 6th ed., 1994.
3. Skoog, D.A., Holler, F.J., Nieman, T.A., Principles of Instrumental Analysis, 5th ed., Thomson
Brooks/Cole, 1998.
4. Christian, G. Analytical Chemistry, 6th ed., John Wiley & Sons, 2006.
5. Harris, C.H., Quantitative Chemical Analysis, 6th ed., W.H. Freeman and Co. New York, 2003.

Week Chapter Details Activities Laboratory Activities

1 Electromagnetic Online Laboratory Online
Oct 12 - 16 radiatio Lecture briefing,
Chapter 1:
Learn
Introduction to
the
Spectroscopic
operation of
Method Analysis
2 Spectroscopic Online Exp 1 - Exp 4 Online
Oct 19 - 23 measurements Lecture (SWR)
3 Instrumentation Online Exp 1 - Exp 4 Online
Oct 26 - 30 Lecture (SWR)
4 UV and visible Online Exp 1 - Exp 4
Chapter 2:
Nov 2 -6 spectroscopy Lecture
Ultraviolet/Visible
Absorption
5 Procedural Online Exp 1 - Exp 4
Spectroscopy
Nov 9 - 13 details, Lecture
quantitative Test
analysis
6 Theory of infrared Online Exp 5 - Exp 6 Online
Nov 16 - 20 absorption, Lecture (SWR)
Chapter 2:
sample
Infrared
handling
Absorption
techniques
Spectroscopy
7 Qualitative Online Exp 5 - Exp 6
Nov 23 - 28 applications Lecture
8 Fundamental Online Optional Lab Online
Dec 7 - 11 principles Lecture Session (SWR)
of atomic
absorption
spectroscopy
Chapter 3:
Instrumentation
Atomic
9 Interferences Online
Spectroscopy and
Dec 14 -18 Quantitative Lecture
Flame
analysis,
Atomization
standard addition
method
Flame emission
Spectroscopy
 Week Chapter Details Activities Laboratory Activities
10 Migration rates; Online Online
Dec 21 - 25 capacity factor, Lecture (SWR)
Chapter 4: An
selectivity factor,
Introduction to
column efficiency,
Chromatographic
qualitative/quanti
Separations
tative
analysis
11 rinciples, Online Online
Dec 28 – 1 Jan instrumentation Lecture Presentation
(injection port,
Chapter 5: Gas columns, oven
Chromatography temperature)
12 GC Detectors: Online
Jan 4 - 8 Principles and Lecture
applications
13 Principles and Online Online
Chapter 6: High
Jan 11 - 15 instrumentation Lecture Presentation
Performance
14 Types of liquid Online
Liquid
Jan 18 - 22 chromatography, Lecture
Chromatography
applications
15
Study Week
Jan 25 -31
Feb 1 - 23 FINAL ASSESSMENT