List of specialised equipment required for G10 Biology

Practical Essential Desirable

3.2 Prepared slides of Amoeba,
Paramecium, Euglena, Volvox,
3.3 Prepared slides of Spyrogira and
Diatoms
4.3 Mounted needles
6.2 Iodine solution (In KI)
7.1 Salt (NaCl), washing up liquid,
rubbing alcohol, flashlight,
blender, strainer, coffee filter
7.2 Prepared slides of Alium (onion) and
Ascaris (parasite)
8.3 Petri dishes
10.1 Thermometers class set.-5-105C
vinegar, baking soda,
11 Earthworms. Dissection kit, tray
with wax, pins, scalpel, forceps,
scissors
13. Dissection equipment and small fish
from lake
14.2 White vinegar, ammonia
containing cleaning liquid,
beakers, boiling pan and red
cabbage.
14.3 Slides of cross section of plant
stems of Corn and Buttercup (Zea
mays and Ranunculus)
15.1 Sharp scissors, scalpels
15.2 Sharp knives (cooking)

HRW 456 400ml beakers class set, ice, hot

water, thermometers, u-shaped
glass tubing 30 cm long, 125ml
beaker class set, culture of
paramecium, hand lenses, stop
clocks, glass marking pen or
chinagraph pencil
HRW 934 Stop watch or stop clock class
set
Heart to dissect.
Dissection kit
HRW 954 5litre plus plastic transparent
container,
rubber tubing,
washing up bowl,
water resistance marker pen
HRW 1032 100ml beakers, class set x4,
gelatine, class set x2.5g,
measuring cylinders 100ml class
set,
vitamin capsules, class set,
cooking oil 75ml x class set,
Plastic spoons class set
HRW 28 Light and dark coloured sand,
100ml graduated cylinders class
set,
Celsius thermometers -5 – 105C
class set,
plastic cups,
graph paper,
stop clock,
W&D Expt 5.3 Fresh pineapple,
The fragility of cheese grater,
an enzyme 3 x100ml beakers,
p152-153 gelatin/jello,
stove,
fridge,
2 spoons
4. Identifying Iodine solution,
organic Benedicts solution, or copper
compounds in sulphate solution and Sodium
foods: Bread, Hydroxide solution.
cheese, potato, Biuret solution
rice, palm oil. Brown paper or card
Use iodine test, Stove
Benedicts test, Beakers
Biuret test, Test tubes
grease spot test. Goggles
(ACR will send Food samples
details)
W&D p 30 Expt Droppers
1.2 Glass slides
Coverslips
Bright thread
Examining Slides
animal cheek Coverslips
cells Cotton swabs
Methylene blue
 Microscopes
W&D Ex 2.2 p60 Microscopes
Slides
Coverslips
Droppers
Pondwater
Absorbent paper/blotting paper
Comparing 30 cm long rulers(Metal preferably)
mean size of Or calipers class set
e.g. shell Number of shells for measuring all
lengths (ACR to of the same species
send). Calculate Alternative, plant seed pods of the
mean size and same species
standard
deviation.
HRW p98 Disposable gloves, class set
Lab coats
Safety goggles
600ml beaker
25cm dialysis tubing
Funnels
Starch
Lugols solution
100ml graduated cylinder
20cm piece of string

HRW p 110 Safety goggles

analysing the Lab coat
effect of cell size Disposable gloves
on diffusion Block of phenol phthalein agar
Each student needs a block
3x6x2cm
Scalpels class set
Beakers 250ml class set
1.2 litres vinegar
Plastic spoon
Paper towel
Test tubes 5x class set
W&D p197 DNA Blender
Extraction Toothpicks
Liquid soap
Salt
Plastic strainer
Meat tenderiser(pineapple)
Beaker
Rubbing alcohol
List of specialised equipment required for G11 Biology

Extraction of essential equipment needed

Safety apparatus
Goggles/safety glasses
Lab coats/aprons
Disposable gloves box
Long protective gloves
Dustpan and brush

Class apparatus
Water heaters -class set, to enable students to have water baths at different temperatures
for enzyme experiments and other experiments e.g. water baths (electricity), gas bottle
stoves etc
Blender 2
Stop clocks 12
Fridge 1
Paper towels 1
Mop and bucket, long handled brush, 1
box/bucket for broken glassware 1
Washing up bowls (dirty glassware) 4
Deep sided trays (for holding class sets of apparatus >10
Chinagraph pencils
Rubber tubing
Filter papers

Glassware
Beakers 400 ml 20
250 ml 80
100 ml 20
Measuring cylinders 100 ml 20
Boiling tubes 200
Test tubes 100
Glass bowls straight sided 3cm height. 8 cm diameter approx50
U shaped glass tubes with 2 bungs 12
Funnels (plastic) 12
test tube racks (can make ) 12

Liquids and solutions

Lugol’s iodine (potassium iodide and iodine)
Benedicts reagent (sodium carbonate, sodium citrate and copper sulphate)
Iodine solution
Biuret reagent (potassium sodium tartrate, copper 2 sulphate, sodium hydroxide)
Rubbing alcohol
Acetic acid
Ammonia solution (Ammonium hydroxide solution 10%-25%)
Cooking oil (palm oil ok)
pH Universal indicator solution
phenolphthalein solution

Solids
Sodium hydroxide NaOH
Sodium chloride NaCl
Baking soda NaHCO3
Gelatin /jello
Agar
Starch
Glucose
Sucrose

Dissecting apparatus
Sharp pointed scissors 15
Scalpels 15
Fine forceps 15
Blunt forceps 15
Mounted needles 15
Blunt ended probes 15
Dropping pipettes 40
Plastic 5 ml syringes 20

Assorted equipment
Metal rulers 15
Plastic spoons 15
Thermometers -5C – 105 C 40
Cheese grater 1
Sieves 1
Petri dishes 50

Making fresh reagents

Biuret REAGENT according to Gornall et al. (1949)
1. Weigh 1.50 g of cupric sulfate pentahydrate (CuSO4 5 H2O) with 6.0 g sodium potassium
tartrate tetrahydrate (NaKC4H4O6 4 H2O).

2. Dissolve in 500 ml of H2O.

3. Add 300 ml of 10% NaOH.

4. Make up to total volume of 1 liter. Store in a plastic bottle protected from light.
Gornall, A.G., Bardawill, C. J. and David, M. M. (1949)
Determination of Serum Proteins by means of the Biuret Reaction.
J. Biol. Chem. 177; 751.