FAKULTI SAINS GUNAAN

UNIVERISITI TEKNOLOGI MARA

CAWANGAN PERAK KAMPUS TAPAH

BIO150 – METABOLISM & CELL DIVISION

SCIENTIFIC LAB REPORT

NAME:

GROUP: AS1202B

EXPERIMENT TITLE: ENZYME 1: THE FASTEST ENZYME-CATALASE

LECTURER: SIR MOHAMAD AZAM AKMAL ABU BAKAR

DATE OF SUBMISSION: 6 JUNE 2022

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Declaration of Academic Honesty

Academic honesty or academic integrity is a very important virtue that all students should
uphold at all times.

I declare that the lab report submitted is not plagiarised and is entirely my own work, and
that no part of it has been copied from any work produced by other person(s)/ source(s) or
provided by any other student(s).

I understand that issuing a false declaration can result in severe penalties and I am willing to
be penalized if any form of copying found valid.

EXPERIMENT 1
INTRODUCTION
The catalase enzyme breaks down hydrogen peroxide (H2O2) into water and oxygen.
2 H 2 O 2 CATALASE 2 H 2 O+O 2
→

Hydrogen peroxide is a highly active chemical formed continually as a by-product of

chemical reactions in living cells. It is poisonous and if it were not immediately broken down
by the cells, it would destroy them. Hence, the importance of the enzyme is known.

OBJECTIVE
1. To identify the factor influence the reaction of catalase enzyme.
2. To analyse the reaction of catalase on different samples.

HYPOTHESIS
1. The smaller the area of the surface, the higher the presence of the oxygen.

APPARATUS
1. Fresh sample: -Liver
-Potato
2. 7 test tubes
3. Pestel and mortar
4. Beaker 250 cm3
5. Thermometer
6. Bunsen burner
7. Hydrogen peroxide

PROCEDURE
1. 2cm3 hydrogen peroxide solutions are poured into a clean tube. 1cm cube of liver cut
and dropped into the test tube of hydrogen peroxide. The samples was carefully
observed and recorded what happens. The present of oxygen tested with a splint.
2. A piece of liver with the same size as before was taken and placed it in mortar. The
liver grinded and then the ground liver transferred into a test tube containing the
fresh hydrogen peroxide. The activity of the ground of liver compares with the
activity observed for the whole piece of liver noted.
3. Another piece of liver was put in a beaker of boiling water for three minutes. Then
drop the piece of liver into fresh hydrogen peroxide and find out if the enzyme is still
capable of breaking down the hydrogen peroxide.
4. The experiment was carried out using the other fresh example: potato to find out if it
contained catalase enzyme.
5. The result was recorded in the table1.

Table 1: Catalase Reaction

Test tube containing Observations Presence of Oxygen

Cube of liver A little bubble formed on the surface Oxygen is presence

Ground liver More bubbles formed on the surface Oxygen is presence

Boiled liver No bubbles formed on the surface Oxygen is absent

Cube of potato A little bubble formed on the surface Oxygen is presence

Ground potato More bubbles formed on the surface Oxygen is presence

 Boiled potato No bubbles formed on the surface Oxygen is absent

DISCUSSION
The existence of oxygen was demonstrated by the reaction producing bubbles. The higher
the oxygen content, the more bubbles there are. When compared to pulverised liver and
potato, the cubes of liver and potato displayed a little bubble. The enzyme reacts faster to
make oxygen since the surface is smaller, resulting in more bubbles on the surface. Because
the enzyme functions at an ideal temperature of 37°C-39°C, and when it surpasses this
temperature, the enzyme stops working and begins to break down, there were no bubbles
on the surface of the boiled live and boiled potato.
CONCLUSION
The theory has gained traction. When the sample size is lower, the reaction occurs more
quickly. However, the amount of oxygen generated when the sample was cooked was so
small that we may not be able to detect it. It's safe to presume it's zero. This experiment
was successful in determining the factors that influence the catalase enzyme responses. The
experiment's objective has been met. Except the findings for the ground potato were all
correct and revealed a data trend that is correct. It was included to make these findings
even more credible. It would necessitate several repeats of the experiment, because it helps
us in knowing the errors in the experiment.