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Liquid Chromatography Questions Answers
Liquid Chromatography Questions Answers
Liquid Chromatography Questions Answers
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Author: userr
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Answer: a
a) Pulsed output
b) Dependent on solvent viscosity
c) Dependent on back pressure
d) Inconvenient for solvent gradient elution
Answer: a
Explanation: Pneumatic pumps provide pulse free output. They are inexpensive.
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3. Which of the following is not a desired characteristic of pulse dampers or flow
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smootheners used in liquid chromatography?
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a) Easy mobile phase change over
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b) Constant flow must be maintained
c) Should be effective at low system pressureTo
d) Maximal dead volume
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Answer: d
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Explanation: Pulse dampers are used to avoid variations in flow. They must have minimal dead
volume.
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4. Which of the following will improve the efficiency of separation process in liquid
chromatography?
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Answer: d
Explanation: Reduction in sample size and reduction in column diameter will improve the
efficiency of separation process in liquid chromatography. The effect of uneven flow will also be
reduced.
5. Which of the following are the practical problems that arise due to the decrease in
column diameter?
Answer: c
Explanation: The practical problems that arise due to decrease in column diameter are
requirement of small particle size and high pressure drop. But, it increases column efficiency.
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6. Which of the following is not true about guard column used in liquid chromatography?
Answer: c
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Explanation: Guard column removes particles that cause precipitation upon contact with
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stationary or mobile phase. It is placed before the separation column.
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7. Which of the following columns are not used in liquid or high performance liquid
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chromatography?
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a) Analytical column
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b) Separation column
c) Guard column
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d) Capillary column
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Answer: d
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Explanation: Capillary column is used in gas chromatography. Analytical column is also known
as separation column.
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a) Gel permeation
b) Ion exchange
c) Liquid-solid
d) Paper
Answer: d
9. Which of the following is not true about radial compression column when compared to
standard separation column?
Answer: a
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Explanation: Radial compression columns have a wider diameter than standard separation
column. The cartridges used are of low cost.
10. Which of the following is not true about narrow bore column when compared to
standard columns?
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d) Detector response time increases
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Answer: d
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Explanation: Detector response time increases in narrow bore columns. High purity solvents
must be used in these columns. To
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11. Which of the following types of liquid chromatography uses immobilized biochemical
as stationary phase?
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c) Affinity chromatography
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Answer: c
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