™

APS ONE

User Manual
Original Instructions
161150-1329 - G - en - 2020-09 - 161150-1600

Industry Use - EN
Table of Contents

Introduction to the Instrument...............................................................................1-1

Intended Use and Users.............................................................................................. 1-1
Benefits and Limitations of Use................................................................................... 1-1
Warning and Safety Messages.................................................................................... 1-1
Statement Types............................................................................................... 1-1
General Warnings........................................................................................................ 1-2
General Statements..................................................................................................... 1-2
Standard Symbols........................................................................................................1-5

Safety Information...................................................................................................2-1
System Compliance..................................................................................................... 2-1
Instrument Labels........................................................................................................ 2-1
Safety Precautions.......................................................................................................2-2

Instrument Description and Basic Operations.....................................................3-1

Instrument Description................................................................................................. 3-1
Instrument......................................................................................................... 3-1
Operating Area..................................................................................................3-2
User Interface....................................................................................................3-2
Screen...............................................................................................................3-3
Connection Panel..............................................................................................3-3
List of Accessories....................................................................................................... 3-4
List of Consumables.................................................................................................... 3-5
Technical Data and Specifications............................................................................... 3-5
Plates................................................................................................................ 3-6

Instrument Installation and Configuration............................................................4-1

Unpacking (Checking for Damage)..............................................................................4-1
Installing the Instrument...............................................................................................4-2
Installing the Dispensing Tubing.................................................................................. 4-3
Installing the 2nd Pump Kit.......................................................................................... 4-5
Installing the 55 mm Dishes Adaptation Kit................................................................. 4-7
Installing the 90 mm Dishes Adaptation Kit................................................................. 4-7
Installing the LINX Printer Kit....................................................................................... 4-8

Workflow and Instructional Procedures............................................................... 5-1

Starting the Instrument.................................................................................................5-1
Shutting Down the Instrument......................................................................................5-1
Managing the Programs...............................................................................................5-1
Creating a Program...........................................................................................5-1
Modifying a Program......................................................................................... 5-4
Making a Program Available............................................................................. 5-5
Managing the Operating Parameters...........................................................................5-6
Exporting Data.................................................................................................. 5-7
Managing the Operators.............................................................................................. 5-8
Adding an Operator...........................................................................................5-9
Modifying an Operator.....................................................................................5-10
Deleting an Operator.......................................................................................5-10
Loading and Unloading the Carousel.........................................................................5-10
Starting a Dispensing Cycle....................................................................................... 5-11
Selecting a Program........................................................................................5-11
Interrupting the Cycle...................................................................................... 5-14

161150-1329 - G i APS ONE™

Table of Contents

Ending the Cycle............................................................................................. 5-14

Reloading the Carousel while in Cycle (Continuous Mode).......................................5-14
Using the Pump Mode............................................................................................... 5-16
Dispensing with In-line Addition................................................................................. 5-18
In-line addition Using a 2nd pump kit.............................................................. 5-18
Dispensing in Biplate Mode....................................................................................... 5-19
Dispensing in Turbo Mode......................................................................................... 5-20
Traceability.................................................................................................................5-21
Traceability of a Dispensing Cycle.................................................................. 5-21
Dish Marking................................................................................................... 5-21

User Maintenance................................................................................................... 6-1

Calibration and Adjustments........................................................................................ 6-1
Adjusting the Pump........................................................................................... 6-1
Cleaning Procedures................................................................................................... 6-2
Cleaning the APS ONE™ instrument................................................................ 6-2
Cleaning the Tubing Before Autoclaving........................................................... 6-5
Autoclaving........................................................................................................6-5
Preventive Maintenance Operations............................................................................6-6
Replacing the UV Lamp.................................................................................... 6-6

Appendix - Configuring the LINX 4900 Printer.................................................... A-1

Appendix - Configuring the LINX CJ400 Printer..................................................B-1

Appendix - Troubleshooting..................................................................................C-1
Error Messages........................................................................................................... C-1
Error Messages and Recovery Procedures................................................................ C-2
Symptoms.........................................................................................................C-2

Revision History

161150-1329 - G ii APS ONE™

General Information

The content of this document is based on the Software release 1.0.

Please discard any previous copies of this document if relevant.
This document may contain information or references relating to certain bioMérieux products,
software or services which are not available in the country of release; this shall not mean that
bioMérieux intends to market such products, software or services in such country.
To request copies of publications or for any technical request / assistance, contact bioMérieux
or your local distributor (contact information available on www.biomerieux.com).

Note: The screens and figures shown are intended as illustrations only and must not be interpreted
as actual representations of data, results or equipment.
Screens and equipment are not shown to scale.

IMPORTANT: Please read this document carefully before using the instrument.

Limited Warranty

bioMérieux warrants the performance of the product for its stated intended use provided that
all procedures for usage, storage and handling, shelf life (when applicable), and precautions
are strictly followed as detailed in the instructions for use (IFU).
Except as expressly set forth above, bioMérieux hereby disclaims all warranties, including any
implied warranties of merchantability and fitness for a particular purpose or use, and disclaims
all liability, whether direct, indirect or consequential, for any use of the reagent, software,
instrument and disposables (the "System") other than as set forth in the IFU.
Customer acknowledges and agrees that use of the System for testing of sample types or for
indications other than those described in the IFU is done solely at the Customer’s own risk.
Customer acknowledges and agrees that it is Customer’s sole and exclusive responsibility to
validate the System for any such intended use, and to determine whether the System is
suitable for that intended use. The performance of any validation studies and the subsequent
use of the System based on Customer’s validation studies shall be the Customer’s sole risk
and responsibility.
Product warranty details can be obtained from bioMérieux or your local distributor (contact
information available on www.biomerieux.com).

Intellectual Property

BIOMERIEUX and the BIOMÉRIEUX logo, APS ONE are used, pending, and/or registered
trademarks belonging to bioMérieux, or one of its subsidiaries, or one of its companies.
Any other name or trademark is the property of its respective owner.
© 2020 bioMérieux SA
bioMérieux SA 673 620 399 RCS Lyon
1 Introduction to the Instrument

Intended Use and Users

The APS ONE™ instrument is used to fill Petri dishes with media. It accomodates two sizes of
Petri dishes: 90 mm and 55 mm.
This instrument is to be used for microbiological tests in the food, pharmaceutical, cosmetic
and clinical laboratories and industries.
This equipment is to be used by qualified, trained laboratory professionals in the
aforementioned industries.

Benefits and Limitations of Use

The APS ONE™ instrument optimizes the workflow through lab automation. It enhances:
• Productivity by removing the manual steps to fill up to 650 Petri dishes per hour.
• Reproducibility by preventing human error and ensuring batch-to-batch reproducibility.
• Production of quality culture media plates and the sterility of the dispensing zone ensured
by UV lamp.
• Ease of use by standardizing dispensing, loading quickly and easily.
• Optimal access for cleaning
• Flexibility by using different modes to do online blood addition and additives in-process.
It is a compact and versatile instrument with a user-friendly interface.
The APS ONE™ instrument must be used only for the purposes specified in the instructions
for use.

Warning and Safety Messages

The user documentation uses several types of statements to alert you to important
information. Important information is labeled in text and identified using symbols.

Statement Types
The statement types are Warning, Caution, Important, and Note. The following examples
define each statement type. The general caution symbol is used in these examples, but other
symbols (see Standard Symbols) may be used instead.
The warning messages in this document mainly refer to:

WARNING
A Warning statement alerts the user to the possibility of injury, death, or
other serious adverse reactions associated with the use or misuse of the
device.

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General Warnings Introduction to the Instrument

CAUTION: A Caution statement alerts the user to the possibility of a problem

with the device associated with its use or misuse. Such problems include
device malfunction, device failure, damage to the device, or damage to other
property. Where applicable, a Caution statement may include a precaution
that should be taken to avoid the hazard.

IMPORTANT: An Important statement relates to content presented in the user documentation. It is

used to reinforce the user’s understanding of selected information.

Note: A Note statement supplies additional information about a topic.

General Warnings

See the General Safety and Regulatory Information booklet.

General Statements

This section provides important statements that apply to all products. Equipment meets the
requirements and standards stated in the certificate supplied with it.

WARNING
Equipment is intended for professional use only.
Laboratory personnel should be qualified and adhere to the principles of
good laboratory practice.
All the user documents supplied must be read prior to use of the equipment.
Under no circumstance should the user dismantle equipment due to the risk
of touching dangerous parts, including parts that may be infectious or
connected to a source of electric power.
Do not obstruct the equipment and hardware ventilation apertures, and leave
sufficient clearance around the equipment for the circulation of air.
All biological materials should be considered as potentially infectious.
Suitable individual protective equipment is required when handling chemical
or biological substances.
bioMérieux is in no case liable for the harmful consequences of incorrect use
or improper handling of these substances.

161150-1329 - G 1-2 APS ONE™

General Statements Introduction to the Instrument

WARNING
Electromagnetic Compatibility (EMC):
The EMC class of the equipment is indicated on the certificate supplied with
it.
If equipment is a class A product, it may cause radio interference in a
domestic environment, in which case the user will be required to correct the
interference at his own expense.
Do not use this device near strong sources of electromagnetic radiation (for
example, intentionally unprotected radio-electric sources), which could
interfere with the operation of the equipment.
It is recommended to evaluate the electromagnetic environment before
starting the device.

WARNING
The user is responsible for the installation of antivirus software and
Windows security updates (Microsoft Monthly Security and Quality Rollups)
on bioMérieux Windows platforms, in order to keep Microsoft operating
systems and software components secure.

WARNING
In order to avoid computer viruses or abnormal functioning of your
equipment, never download any software other than those ensuring the
protection of your network and those provided or recommended by
bioMérieux.
It is your responsibility to secure your network and ensure this protection is
appropriate and maintained. It is recommended to use all appropriate means
(including antivirus software, security patches, firewall) to protect your
network from virus intrusion, unauthorized use, alteration, manipulation and
disclosure.
In an effort to reduce the risk of spreading a virus to bioMérieux equipment,
it is recommended that only bioMérieux supplied USB devices are used with
bioMérieux equipment. The use of personal USB devices is not
recommended. To avoid computer viruses and the potential loss of
functionality and/or results, use caution when transferring USB devices
between computers. Do not use USB devices intended for bioMérieux
equipment in other computers that do not have current antivirus software
installed and active.
All computer media (CD, DVD, USB key) supplied with this equipment should
be stored and stocked in a suitable location.
Only modify the software configuration parameters you are authorized to
modify and which are described in the user documentation.

161150-1329 - G 1-3 APS ONE™

General Statements Introduction to the Instrument

WARNING
Decontamination of equipment at the end of its life cycle:
The following instructions must be followed by all users in countries where
local legislation imposes the treatment and recycling of equipment at the end
of its life cycle.
As a general rule, and as a precautionary measure, any part of the equipment
(including sub-assemblies, components and materials) considered to be
potentially infectious, must be decontaminated, whenever possible, or
removed if decontamination is impossible or presents a risk.
Any part considered to be potentially infectious, which is not
decontaminated, must be removed from the instrument before following the
normal channels for elimination of infectious products, in accordance with
local regulations.
The decontamination instructions in the user documentation correspond to
the parts of the equipment that are potentially infectious according to their
intended use. These operations must be performed before the equipment is
transferred to a third party.
However, bioMérieux cannot exclude that other parts of the equipment have
not been contaminated in other circumstances, in particular as the result of
spillage of infectious substances. In this case, the user is solely responsible
for decontaminating these parts or removing them before they follow the
normal channels for elimination of infectious products.

WARNING
This statement only applies to European countries with regard to the waste
electrical and electronic equipment European directive:
You can play an important role in contributing to reuse, recycling, and other
forms of recovery of waste electrical and electronic equipment. Sorting this
type of waste significantly reduces potential negative effects on the
environment and human health as a result of the presence of hazardous
substances in electrical and electronic equipment.
At the end of the life cycle of this product, do not dispose of the product as
unsorted municipal waste, even if it is decontaminated. It is imperative that
you contact bioMérieux to assure its appropriate disposal.

IMPORTANT: Electrical or other connections should only be made using the accessories supplied
with the equipment.

IMPORTANT: It is important to follow all the restrictions on use, particularly concerning

temperature, storage, installation and voltage, which are indicated on the product
label or in the user documentation.

161150-1329 - G 1-4 APS ONE™

Standard Symbols Introduction to the Instrument

IMPORTANT: The accuracy of results obtained with this equipment depends on the maintenance
operations described in the user documentation (user maintenance and/or periodic
preventive maintenance performed by bioMérieux).

IMPORTANT: The user should be aware that if the maintenance operations are not performed, are
only partially performed, or are not performed as described in the user
documentation, bioMérieux is in no case liable for any false test results obtained.

IMPORTANT: It is recommended to keep the original packaging materials in case the equipment
needs to be moved. Any damage directly or indirectly resulting from the transport of
the equipment without adequate containers will not be covered by the warranty.

Standard Symbols

The following table defines symbols that may appear in the instructions for use or on the
instrument, package inserts, or packaging. When surrounded by a triangle on a yellow
background, the symbol highlights an immediate warning and is positioned on the instrument
itself.

Compliance with US and Canadian

CE-Marking of Conformity
Safety Standards certified by CSA

Compliance with China RoHS

UL Listed to US and Canadian Safety
Regulation (Chinese Standard SJ/
Standards
T11364)

Eurasian Conformity

Authorized Representative in the

Batch code
European Community

Catalogue number Serial number

Consult Instructions for Use Use by date

Manufacturer Date of manufacture

This way up Do not stack

Caution, consult accompanying

Contains sufficient for <n> tests
documents

Do not reuse Keep dry

161150-1329 - G 1-5 APS ONE™

Standard Symbols Introduction to the Instrument

Keep away from light Fragile, handle with care

Humidity limitation Keep away from magnetic field

Temperature limitations Upper limit of temperature

Lower limit of temperature Sterile

Positive control Negative control

Biological risk Electric shock warning

Radiation warning Hot surface

Potential pinch-point warning Laser beam

Shearing hazard High temperature

Hazardous magnetic field Potential tip-over/crush hazard

Automatically moving mechanical parts

Acute toxicity Corrosives

161150-1329 - G 1-6 APS ONE™

Standard Symbols Introduction to the Instrument

Sodium azide Irritant

Separate collection for waste electrical

Recyclable
and electronic equipment

Environmentally friendly use period.

Actual number of years may vary by
product. This symbol is typically
orange in color.

Fuse Direct current

Alternating current Both direct and alternating current

Three-phase alternating current Earth (ground) terminal

Protective conductor terminal Frame or chassis terminal

Equipotentiality ON (supply)

ON (only for a component of the

OFF (supply)
system equipment)

Equipment protected throughout by

OFF (only for a component of the double insulation or reinforced
system equipment) insulation (Equivalent to Class II of IEC
536)

Ethernet port

161150-1329 - G 1-7 APS ONE™

2 Safety Information

It is essential that the warnings, cautions and safety requirements contained in this document
are read and understood by the user before operating the system.
Warning symbols have been placed on the system to draw your attention to areas of potential
hazards.
System Compliance

This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference in which case the user will be required to correct
the interference at his or her own expense.
PLEASE NOTE: Any changes or modifications not expressly approved by the party
responsible for compliance could void the user’s authority to operate the equipment.

Industry Canada (IC) Compliance

This Class A digital apparatus complies with Canadian ICES-003.

EC Complance
This equipment complies with the 2004/108/CE and 2006/95/CE directives.

Instrument Labels

Note: The picture of the rating plate is provided for information purposes only. The marks of
conformity printed on it depend on the current regulations in each country in which the
instrument is marketed.

This device complies with Part 5 of the FCC

Rules. Operation is subject to the following
two conditions: (1) this device may not cause
harmful interference, and (2) this device must
accept any interference received, including
interference that may cause undesired
operation.
CAN ICES-3 (A) / NMB-3 (A)
INPUT: 110-120V/220-240V 50/60Hz, 240W

161150-1329 - G 2-1 APS ONE™

Safety Precautions Safety Information

Safety Precautions

Pay particular attention to the following safety precautions. If these safety precautions are
ignored, injury to the operator or damage to the instrument may occur. Each individual
precaution is important.

WARNING
If the equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.

WARNING
All biological samples and quality control (QC) products incubated in this
system, as well as all waste in the waste containers, should be treated as
potentially biohazardous materials. All materials and mechanical
components associated with the waste systems should be handled
according to safe microbiological practices in compliance with the
installation site's biohazard procedures. Use the personal protective
equipment recommended by the facility when handling any of these
components, including gloves, safety glasses, and a lab coat.
The system must be decontaminated before its covers are removed by
service personnel. Ensure that appropriate decontamination is carried out if
hazardous materials are spilled on or into the equipment or surrounding
areas.

WARNING
Treat waste material, including consumable items, and any components
coming into contact with waste material as having the potential hazards of
the samples used.
All service personnel should be familiar with the Material Safety Data Sheet
(MSDS) for all materials used in the procedures relating to this instrument,
and the correct procedures for handling these materials.

WARNING
Even when power is removed from the instrument, the potential exists for
electricity to be generated if components, such as assemblies that are
mounted on belts, are moved too quickly. Components should be moved
slowly to prevent the buildup of electricity. Failure to comply may result in
injury to personnel or damage to the instrument.

161150-1329 - G 2-2 APS ONE™

Safety Precautions Safety Information

WARNING
Do not replace detachable main supply cords by inadequately rated cords.
Only use main supply cords provided by the manufacturer.
Do not replace electric cables. If cables do not have the same technical
specificities, there is a risk of electrical shock.

WARNING
Electronic equipment can be the source of electrical shocks. Installation,
service, and repair should only be performed by authorized and qualified
bioMérieux personnel.

WARNING
All power switches should be off when connecting or disconnecting cables
to power outlets to reduce the risk of electrical shock.

WARNING
bioMérieux recommends connecting this instrument to a main power outlet
that is protected with a ground fault circuit interrupter to reduce the risk of
electrical shock.

WARNING
As with any mechanical system, certain precautions must be taken when
operating the instrument. The instrument has a protective cover intended to
prevent the operator from coming into contact with any moving parts and
aerosols. When servicing the instrument, take special care, as there are
moving parts that can cause injury.

WARNING
Cleaning and disinfecting solutions have corrosive properties. Always wear
protective (chemical resistant) gloves and safety glasses when handling
cleaning and disinfecting solutions.

WARNING
Hot surfaces can cause injury.

161150-1329 - G 2-3 APS ONE™

Safety Precautions Safety Information

CAUTION: Any liquid spilled on the system may result in system

malfunctioning. If liquid is spilled on the system, wipe it up immediately
using decontamination wipes.

CAUTION: The computer and its operating system have been carefully
configured for optimal system performance. Altering the configuration may
severely hamper the usability of the instrument.

Note: Before performing electrical safety or other compliance testing on the instrument, contact
bioMérieux or your local distributor.

WARNING
The user must only perform the maintenance operations described in this
document and rigorously follow each of the steps.
The use of tools not specified by bioMérieux is forbidden.
Powder-free gloves, a lab coat and protective glasses or goggles must be
worn when performing maintenance operations.
Always use personal protective equipment, including gloves, a lab coat and
safety glasses or goggles when handling reagents.

WARNING
Electromagnetic Compatibility (EMC):
The EMC class of the equipment is indicated on the certificate supplied with
it.
If equipment is a class A product, it may cause radio interference in a
domestic environment, in which case the user will be required to correct the
interference at his own expense.
Do not use this device near strong sources of electromagnetic radiation (for
example, intentionally unprotected radio-electric sources), which could
interfere with the operation of the equipment.
It is recommended to evaluate the electromagnetic environment before
starting the device.

161150-1329 - G 2-4 APS ONE™

3 Instrument Description and Basic Operations

Instrument Description

Instrument

Number Part name

1 Carousel
2 Connection Panel
3 User Interface
4 Safety Cover
5 Operating Area

161150-1329 - G 3-1 APS ONE™

Operating Area Instrument Description and Basic Operations

Operating Area

Number Part name

1 Pistons
2 Guiding plate
3 Dispensing nozzle holder
4 Shuttle
5 UV lamp
6 Pump

User Interface

Number Part name

1 Screen
2 Keys for menu selection

161150-1329 - G 3-2 APS ONE™

Screen Instrument Description and Basic Operations

Number Part name

3 Navigation pad
The 4 arrows on the paddle are used to
move around the screen.

4 Validation key
The middle key is used as a validation key
and to enable the alphanumerical
keyboards.

Screen

Number Part name

1 The screen title indicates the status or the
step the unit at.
2 An informative or warning message
appears, depending on the case.
3 Data that can be modified turns orange.

Menus that can be accessed are displayed as buttons. You may open them by pressing the
A, B, C and D keys under displayed buttons.
Alphanumerical keyboards appear on the screen to allow data input. The Navigation pad is
used to select characters.

Connection Panel
On the left side of the APS ONE™ instrument you can connect various elements that add
functionalities.

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List of Accessories Instrument Description and Basic Operations

Number Part name

1 USB ports
2 Ethernet network
3 Communication port
4 Foot switch
5 Alarm device
6 Dish printer
7 External pump

List of Accessories

To get ordering information, contact bioMérieux or your local distributor (contact information
available on www.biomerieux.com).
KIT 55MM PLATES
TWIN PUMP HEAD
APS One 90 mm Tubing set for M10 & M20
APS One 55 mm Tubing set for M10 & M20
APS One 90 mm Tubing set for M28
APS One 55 mm Tubing set for M528
Tubing set standard plate 90
Tubing set standard plate 55
Tubing set turbo plate 90 MM
SECOND PUMP KIT

161150-1329 - G 3-4 APS ONE™

List of Consumables Instrument Description and Basic Operations

Tubing set for blood addition

Biplate kit
NOZZLE HOLDER FOR TALL PLATE
LINX PRINTER FITTING KIT
IQ/OQ QUALIFICATION PROTOCOL
Shuttle D for 55 mm plates
Shuttle C for 55 mm plates
Dispensing nozzle holder
Footswitch for APS One (pump mode)

List of Consumables

To get ordering information, contact bioMérieux or your local distributor (contact information
available on www.biomerieux.com).
Consumable package
UV lamp
Aseptic petri dishes 55 mm
Irradiated petri dishes 90 mm
Aseptic petri dishes 90 mm

Technical Data and Specifications

90mm plates
Installation Conditions Height Up to 2000 metres
Room temperature From 15 to 45°C max.
Relative humidity (RH) From 10 to 80%
Pollution level 2
Atmospheric pressure 700 hPa to 1,100 hPa
Category Type II according the CEI664
directiveP
Power supply 100-240 V ±10% ; 50-60 Hz
Power input 200 W max.
Fuse rating 2 x 2AT
Number of recordable programs 20
Number of recordable users 10

161150-1329 - G 3-5 APS ONE™

Plates Instrument Description and Basic Operations

90mm plates
Unit dimensions (W x H x D) Equipment alone : 720 x 640
x 900 mm
Equipment packed (2
boxes) :
• 805 x 795 x 385 mm
• 760 x 760 x 690 mm

Weight Equipment alone : 60 kg

Equipment packed : 70 kg
Size of the Carrousel (W x H x D) 60 cm x 73 cm x 70 cm
Accuracy 2%
Capacity* Standard carrousel 560 plates
Plates/hour (18 ml/plate) Standard pump ±650
Turbo pump ±800
55 mm plates
Capacity* Standard carrousel 560 plates
Plates/hour (9 ml/plate) Standard pump ±800
*Calculated with plates of the brand GOSSELIN

Plates
The APS ONE™ instrument was developped to operate with most of Petri dish types, provided
that dishes comply with some technical features, as detailed below:

Dimensions of Petri dishes including stacking ring (mm)

Shuttles Lid Bottom
b Hb d Hd
A 91.0< b <92.3 7.4< Hb < 9.0 < 88.8 < 17.5
B 92.0< b <93.3 7.4< Hb < 9.0 < 89.8 < 17.5
C 58.0< b <59.3 9.0< Hb <10.5 < 55.8 < 17.5
D 57.0< b <58.3 7.4< Hb < 9.0 < 55.3 < 17.5

Note: • In case of «deep» dishes (17.5 < Hd < 24.0), please use the nozzle holder for tall plate.
• In case of large dishes, please use the appropriate option. Contact bioMérieux Technical
Assistance or your local distributor.

161150-1329 - G 3-6 APS ONE™

4 Instrument Installation and Configuration

Unpacking (Checking for Damage)

IMPORTANT: A minimum of two people are required to install the instrument as the box is heavy.
Use caution when moving the box to avoid injury. Do not install the instrument on
your own. Follow the safety procedures.

1. Make sure that no damage has occured during transportation.

2. Ensure that all of the accessories listed are included.

x1 User's manual CD
x1 Control certificate
x1 Installation guide
x1 Plate guiding stick
x1 Main supply cable
x2 Spacer adjustment 1 mm
x2 Spacer adjustment 0,7 mm
x2 Standard tubing sets (without priming nozzle)

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Installing the Instrument Instrument Installation and Configuration

x1 Tubing set for PM05 use

x80 Plastic cups
x1 General safety

Installing the Instrument

The unit has been unpacked and assembled according to the assembling procedure.

1. With help, place the APS ONE™ instrument on a flat, horizontal and stable surface (if
possible less than 90 cm high to make the access to the carousel easier.

Note: The instrument has to be installed on a clean and dry work surface able to support
100 Kg.

2. Check that the triangle on the power switch points to the white dash.

161150-1329 - G 4-2 APS ONE™

Installing the Dispensing Tubing Instrument Installation and Configuration

IMPORTANT: When delivered, the cursor at the back of the instrument is positionned for
using the unit with 220-240V power supply.
When plugging in the unit, make sure that it is connected to the ground (earth).

3. Plug the power cable in the instrument, then into a grounded outlet next to the unit.

Note: The user must be able to easily unplug the power cable to deconnect the APS ONE™
instrument from the electrical source if needed.

Installing the Dispensing Tubing

1. Install the priming nozzle on the unit containing the medium to be dispensed.
2. Insert the dispensing nozzle fully into the nozzle holder.

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Installing the Dispensing Tubing Instrument Installation and Configuration

3. To install the tubing on the pump, turn the knob so that the largest tubing inner
diameter is selected.

4. Insert the tubing into the pump.

5. Close the pump cover.

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Installing the 2nd Pump Kit Instrument Installation and Configuration

Table 2: Recommended tubing lenghts

Priming device - pump Pump tubing Pump - Dispensing

device
150 cm 16 cm 60 cm
6. Make sure that the tubing is placed properly.

Number Name
1 Front View
2 Upper View

Installing the 2nd Pump Kit

Following the installation of the 2nd Pump kit, it is advised to adjust the pumps (see Adjusting
the pump).
1. Unscrew the side pannel using the Allen key delivered with the kit.

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Installing the 2nd Pump Kit Instrument Installation and Configuration

2. Replace the original panel with the 2nd Pump addition.

3. Screw the pannel back using the Allen key.

Related Links
Adjusting the Pump

161150-1329 - G 4-6 APS ONE™

Installing the 55 mm Dishes Adaptation Kit Instrument Installation and Configuration

Installing the 55 mm Dishes Adaptation Kit

1. Ensure that the piston heads are placed in the lower position before installing the kit.
2. Assemble the adaptation kit for 55 mm dishes as shown below:

Installing the 90 mm Dishes Adaptation Kit

1. Ensure that the piston heads are placed in the lower position before installing the kit.
2. Assemble the adaptation kit for 90 mm dishes as shown below:

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Installing the LINX Printer Kit Instrument Installation and Configuration

Installing the LINX Printer Kit

1. Remove the carousel.

2. Tilt the APS ONE™ instrument backward.
3. Remove the two protection covers using the Allen key delivered with the kit.

4. Fix the LINX printer fitting kit as shown.

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Installing the LINX Printer Kit Instrument Installation and Configuration

5. Insert the printer head. Make sure that the slot of the printer head forms a 90° angle
with the unit’s upper plate.

6. Make a mark on the printer head. This will help you in positionning the head next time
you use the printer.
7. Connect the printer to the APS ONE™ instrument using the connection cable.

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Installing the LINX Printer Kit Instrument Installation and Configuration

See Managing the Operating Parameters to enable dish printing.

Related Links
Managing the Operating Parameters

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5 Workflow and Instructional Procedures

Starting the Instrument

You have:
• Installed the unit next to a wall socket, on a horizontal, stable, clean, and dry surface able
to support 100 kg.
• Plugged in the power cable delivered with the unit.
Press the ON/OFF button to switch the device on.

Shutting Down the Instrument

Press the ON/OFF button to switch the device off.

Managing the Programs

Your APS ONE™ instrument can contain up to 20 programs. Depending on your requirements
you may add programs and then modify them or make them available.

Creating a Program
1. From the main menu, select the Dispensing Programs menu.

2. Press the Validation key.

3. Select an available program.

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Creating a Program Workflow and Instructional Procedures

4. Press the Validation key.

5. Press the Validation key to access the alphanumerical keyboard and enter the name
of the new program.
6. Use the alphanumerical keyboard to enter the name of the new program.

7. Select OK. Then press the Validation key.

8. Use the navigation pad to select the Mode.

9. Press Validation to scroll down the various modes of cycles:

• 1 medium

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Creating a Program Workflow and Instructional Procedures

• 2 media
• in line addition
• available
10. Use the Navigation pad to select all program’s parameters in turn and configure them
(using the Validation key):
• Type of dishes
• Pump mode
• Dispensed volume (for each pump when it is a 2 media program)
• Pump’s speed (or for both pumps when it is a 2 media program)
• Dispensed blood volume when you have chosen a "In line addition" program
• Agar setting time
• Piston rotation (dish rotation while dispensing - may be disabled for a volume
higher than 18 ml)
• Piston pause duration
The table below gives you indications useful to determine your programs’ parameters.

Mode 1 medium 2 media in line addition

Type of 90 55 biplate 90 55
dishes
Pump Standard / Turbo Standard Standard Standard Standard
Mode
Volume 1 12-13 ml 14-18 ml 5-9 ml 9 ml 12-18 ml 5-9 ml
Pump 1 Standard: Standard: 150 170 180 150
speed 180 180
(rpm)
Turbo: 170

Volume 2 9 ml 1 ml 0.5 ml
(ml)
Pump 2 170 84 60
speed
(rpm)
Piston Yes/No No Yes/No
rotation
Piston 0 to 100 centiseconds
pause
duration*
Agar 0.1 to 99.9 seconds
setting
time

*Piston pause duration: the default value is 10 (100 ms). When dishes go down too
slowly compared to the piston (the dish bottom arriving After the piston), the piston
pause time should be increased.
11. Once all parameters are set, select Programs by pressing the D key to return to the
screen listing programs.

Note: "2 media" and "In line addition" modes are accessible only if the 2nd pump kit has
been installed on your APS ONE™ instrument and the 2nd pump has been enabled in
the Configuring the APS One menu.

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Modifying a Program Workflow and Instructional Procedures

Modifying a Program
1. From the main menu, select Dispensing Programs.

2. Press the Validation key.

3. Select a program number.

4. Press the Validation key.

5. Use the Navigation pad to select the parameters to be modified. Then modify as
required.

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Making a Program Available Workflow and Instructional Procedures

Making a Program Available

1. From the main menu, select Dispensing Programs.

2. Press the Validation key.

3. Select a program number.

4. Press Validate.
5. Use the Navigation pad to select Mode and scroll down the options until Available is
displayed.

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Managing the Operating Parameters Workflow and Instructional Procedures

Managing the Operating Parameters

To adapt the APS ONE™ instrument to your operational requirements, you may configure
some parameters.

Parameter Possible action(s)

Date and time Adjustment
Operating language Selection
Traceability Enabling/Disabling
UV lamp Enabling/Disabling, counter resetting
Logbook Resetting
Dish printing Enabling/Disabling
Refrigerating module Enabling/Disabling
2nd pump Enabling/Disabling
DHCP server Enabling/Disabling
Network parameters IP adress dialling, mask, passerelle
Default number of columns Adjustment

1. From the main menu, select Configuring the APS One.

2. Press the Validation key.

3. Press the Validation key to enter the access code.

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Exporting Data Workflow and Instructional Procedures

4. Type the code 98191. Then select OK.

5. Using the Navigation pad, select the parameter to configure.

6. Press the Validation key.

The configuration screen appears.

Exporting Data
The logbook displays the list of the last hundred events.

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Managing the Operators Workflow and Instructional Procedures

An Export (to USB) button appears, to allow data exportation to a USB key (.txt file).

The Statistics button gives access to the list of events ordered by type. For each event, you
can read the number of occurrences since the last reset of the logbook.

Managing the Operators

You can enter up to 10 operators.

1. From the main screen select Configuring the APS One.

2. Press Validate.
3. Press Validation to enter the access code.

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Adding an Operator Workflow and Instructional Procedures

4. Type the code 98191. Then select OK.

5. Press the B key to enter the Operators menu.

Adding an Operator
1. Using the Navigation pad, select an operator number that is Available (---).
2. Validate.
3. Using the alphanumerical keyboard, enter a name for this new operator.
4. Select OK.

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Modifying an Operator Workflow and Instructional Procedures

Modifying an Operator
1. Using the Navigation pad, select an operator number.
2. Validate.
3. Using the alphanumerical keyboard, modify the name of the selected operator.
4. Validate.

Deleting an Operator
1. Use the Navigation pad to select an operator.
2. Press the A key.
The operator is deleted.

Loading and Unloading the Carousel

You may use the navigation pad to get the carousel turning, and easily reachable column.
1. From the main menu select Carousel loading.

2. Press the Validation key.

3. Use the A and B keys to choose a rotation of one column ( ) or five columns

( ).

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Starting a Dispensing Cycle Workflow and Instructional Procedures

4. Use the Navigation pad to activate the rotation of the carousel :

• to the left
• to the right

Table 3: Indications given when using Gosselin type dishes

Type of carousel Maximal capacity Maximal capacity per

column
Standard 560 40

Starting a Dispensing Cycle

Some empty dishes have been loaded in the carousel. Each column can contain up to 40
dishes.

Selecting a Program
Make sure the dispensing temperature is 42°C and the pump’s speed is adjusted according to
instructions in page 47.
1. From the main menu, select Dispensing Cycles.

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Selecting a Program Workflow and Instructional Procedures

2. Press the Validation key.

3. Select the program.

4. Press the Validation key.

5. You may modify the volume of agar prepared and/or the dispensed volume before
confirming the program.

6. You can start dispensing by pressing the A key to select Dispense.

Before dispensing, you may adjust/calibrate the pump (see Adjusting the Pump) or
prime the pump.

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Selecting a Program Workflow and Instructional Procedures

7. Press the A button to select Pause for interrupting the cycle running.

Note: When traceability is enabled, you can save this cycle data : press the B key to select
Traceability.

Related Links
Adjusting the Pump

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Interrupting the Cycle Workflow and Instructional Procedures

Interrupting the Cycle

Press the A button to select Pause for interrupting the cycle running.
• While in pause, you may modify the agar volume and/or the dispensed volume.
• You also have access to the Calibration menu by pressing the button B.
• You can abort the cycle by pressing the D key.

Ending the Cycle

At the end of the cycle, if your APS ONE™ instrument is connected to a printer, you can print
the dispensing report.

Reloading the Carousel while in Cycle (Continuous Mode)

When dispensed volume is larger than the volume that the carousel can contain, the
operating sequence is different.
When the complet rotation of the carousel is over (14 columns), the screen below appears to
allow reloading:

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Reloading the Carousel while in Cycle (Continuous Mode) Workflow and Instructional Procedures

Note: You may press the B button to select Activate pump 1 to make the pump turn continuously
and avoid agar setting.
• First press: starts the pump
• Second press: stops the pump

There are two options:

1. During the cycle, you have removed filled dishes and replaced them by empty ones.
Make sure that at least one column remains empty so that the cycle may be carried on.
Press the A button to select Resume. The APS ONE™ instrument carries on with the
cycle, using the default number of columns (set in the menu Configuring the APS
One).
2. You are following the reloading procedure.
Press the D button to select Load carousel.
The screen below appears:

1. Press the B or C button to select a one column ( ) or five columns ( )

rotation.
2. Use the navigation pad to activate the carousel rotation:
• to the left

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Using the Pump Mode Workflow and Instructional Procedures

• to the right
3. Remove filled dishes and reload empty dishes.
4. Press the A button to select Columns loaded.
The screen below appears:

5. Press the Validation key on the Navigation pad.

The numerical keypad appears :

6. Enter the number of reloaded columns and press OK.

7. Press the A button to select Validate.
8. The Reloading the carousel screen reappears.
9. The number of reloaded columns has been taken into account.
10. Press the Resume button to carry on with the cycle.

Using the Pump Mode

1. From the main menu select the Pump mode.

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Using the Pump Mode Workflow and Instructional Procedures

2. Press the Validation key.

3. Enter the dispensing parameters.

4. Press the A button to switch to Dispensing mode.

If you selected the MANUAL mode, press the A button to activate dispensing for each
dose or press the foot switch if one is connected.

If you selected the AUTOMATIC mode, the APS ONE™ intrument dispenses doses
according to the pause delay you have previously set.
It is recommended to use the tubing set when dispensing small volumes (9 ml).

161150-1329 - G 5-17 APS ONE™

Dispensing with In-line Addition Workflow and Instructional Procedures

Dispensing with In-line Addition

Dispensing with in-line addition (eg. blood), requires a second pump. For this application you
can install a 2nd pump kit on your APS ONE™ instrument, or connect an external pump (using
the connection cable, see Installing the 2nd Pump).

In-line addition Using a 2nd pump kit

You can perform a dispensing cycle with in-line addition, using a 2nd pump kit and a specific
tubing set. Ensure that the second pump has been actually activated in the Configuring the
APS One menu. Install the dispensing tubing (see Installing the Dispensing Tubing) as shown
below:

Number Name
1 Media preparator

The 2nd pump has been enabled in the Configuring the APS One menu (see Managing the
Operating Parameters). You have created an «in-line addition» program. (see Creating a
Program)
1. From the main menu select the Dispensing Cycles menu.
2. Press the Validation key.
3. Select an "in-line addition" program and fill-in the dispensing parameters (pump speed,
volume to be dispensed).
When you enter the volume to be added, the APS ONE™ instrument will determine the
percentage of the addition and the second pump’s speed. You may unlock this function
to set the pump speed manually. However you must make sure that pump 2 operates
for the same duration as pump 1.
Related Links
Installing the Dispensing Tubing
Managing the Operating Parameters
Creating a Program

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Dispensing in Biplate Mode Workflow and Instructional Procedures

Dispensing in Biplate Mode

Dispensing biplates requires installing a 2nd pump kit (see Installing the 2nd Pump Kit), and a
biplate kit (1 nozzle holder + 2 tubing sets + 2 specific shuttles).
The biplate kit contains the appropriate tubing set. The tubing set is identical to the standard
tubing kit used for 90 mm dishes.
The 2nd pump kit must be enabled in the Configuring the APS One menu (see Managing the
Operating Parameters).
You have created a 2 media program (see Managing the Operating Parameters)
1. Install the shuttle and the specific biplates nozzle holder.
2. Remove the standard shuttle and place the specific shuttle according to the dishes
used (heightened shuttles A and B).
3. Remove the standard holder and replace it by the double nozzle holder.

4. Install the dispensing tubing as indicated on this diagram:

Number Name
1 Autopreparator
2 Autopreparator
5. From the main menu, select the Dispensing cycle menu.
6. Press the Validation key.
7. Select a 2 media program.
8. Enter dispensing parameters (volumes 1 and 2 of agar prepared).

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Dispensing in Turbo Mode Workflow and Instructional Procedures

Volume 1 must be equal to volume 2.

9. Validate parameters by pressing button A.
10. Prime and adjust both pumps.
11. Start dispensing.
Related Links
Installing the 2nd Pump Kit
Managing the Operating Parameters

Dispensing in Turbo Mode

Faster dispensing can be obtained by installing an additional pump on your APS ONE™
instrument. When dispensing 18 ml a rate of 850 plates an hour can be assured.
Volumes from 14 ml may be dispensed with the specific tubing set (Turbo tubing set _ 90 mm
dishes with dispensing nozzle).

Number Name
1 Autopreparator

1. Select a 1 medium program.

2. Fill-in the dispensing parameters (pump speed, volume to be dispensed, pump
configuration).

Note: By default the pump’s speed is set at 170 rprm.

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Traceability Workflow and Instructional Procedures

Traceability

Traceability of a Dispensing Cycle

Traceability functionality has been actually enabled in the Configuring the APS One menu.
When connecting the USB ticket printer to the USB port on the side panel of the unit, you can
edit a dispensing cycle report at the end of the cycle.
At the end of the dispensing cycle simply press the A button to start printing the ticket.

CYCLE REPORT - 1 MEDIUM

01-APS One Serial Nber : 92825557
02-Operator ID : ... (01)
03-Date : (DD/MM/YYYY) 05/10/2009
04-Time (24H) : 13:45:17
------------------------------------
05-Program # : 18 ml
06-MEDIUM 1 : PCA
07-Batch 1 : 2.5.0 249
11-Volume per dish (ml) : 18
14-Dishes quantity : 500
15-Total Volume (ml) : 9000
18-Calibration pmp 1 : 05/10/2009
20-Status : OK

When a USB key is connected to the APS ONE™ instrument, dispensing cycle data is
exported to the key. Then, data can be handled or stored on a PC. The data is
exported when pressing the A key to select Print at the end of the cycle.

Note: It is advised to wait for the end of the installation of the USB Key - 10 to 15 seconds-
before exporting data).

Dish Marking
You can install a printer kit to mark dishes on their side.
Please refer to the installation and user manuals delivered with this kit (LINX CJ400 printer kit
and LINX 4900 printer kit).
Related Links
Installing the LINX Printer Kit
Installing the LINX CJ400 Printer Kit
Appendix - Configuring the LINX 4900 Printer
Appendix - Configuring the LINX CJ400 Printer

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6 User Maintenance

For service information, contact bioMérieux or your local distributor (contact information
available on www.biomerieux.com).
Calibration and Adjustments

Adjusting the Pump

The Adjust menu may be accessed once you have selected a program and you are about to
start dispensing.

Note: Use the C key to prime the pump in order to fill the tubing and remove any air bubbles from
it.

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Cleaning Procedures User Maintenance

Note: If you have installed an optional pump, you may select the pump to be calibrated by pressing
the A button Change pump.

1. Use the B and C keys to Dispense (dispense straight away) or dispense 5 seconds
(dispense within a 5 second delay).
2. Press the Validation key to enable the alphanumerical keyboard.
3. Enter the volume actually dispensed.

4. Then validate.
5. Press the A key to select Save.
Adjustment may be checked by repeating the operation. If so, press the D key to select
Previous screen.

Cleaning Procedures

Cleaning the APS ONE™ instrument

Note: We recommend cleanning thoroughly the unit every day with hot water and 70° alcohol.
The nozzle holder and the shuttle may be removed for an easy cleaning.

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Cleaning the APS ONE™ instrument User Maintenance

IMPORTANT: Do not use scratching pads to clean surfaces.

IMPORTANT: Do not use acetone, organic solvant or pure alcohol to clean the safety cover. You
may damage it.

IMPORTANT: Do not place removable elements in a dishwasher.

IMPORTANT: Following cleaning, make sure that the elements are placed back as follows: the
shuttle is in the front position, the piston is at the guiding plate level, and the carousel
does not interfere with the piston movement.

1. Disconnect the equipment from the wall socket.

2. Remove the dispensing tubes from the equipment.
3. Remove the pump head, taking care not to trap your fingers, and clean it with a cloth
and a cleaning product or hot water.

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Cleaning the APS ONE™ instrument User Maintenance

4. From the main menu, select Cleaning the unit.

5. Press the Validation key.

The screen indicates actions to be done.

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Cleaning the Tubing Before Autoclaving User Maintenance

While actions are being performed, icons turn green progressively.

Note: The waste cup needs to be replaced daily.

Once you have closed the cover, the unit initializes.

With the instrument off, remove the carousel and clean the surface from any dish dust.

Cleaning the Tubing Before Autoclaving

The tubing must be cleaned daily.
Before sterilization, the silicone tubes, the dispensing nozzle and the priming nozzle must be
cleaned.

Note: It is recommanded to change the rotor tubing every 40 hours of use or in case of breakage. It
can vary according to the application and use.

Note: Remove the tube from the pump head after use and at least once a day to avoid damaging
the tubing and to avoid the risk of contamination.

1. Disconnect the priming nozzle from the media source.

2. Press Prime pump to empty the tubes from the remaining diluents.
3. Place the priming nozzle in a container with hot water.
4. Press Prime pump to rinse the tubes, and rinse with distilled or demineralized water.
5. Remove the priming nozzle from the container.
6. Press Prime pump to empty the tubes from the remaining liquid.

Autoclaving
The complete tubing set (silicone tubes, the dispensing nozzle and the priming nozzle) must
be decontaminated by autoclaving.
1. Check that the tubes, the dispensing nozzle and the priming nozzle are empty.
2. Assemble the tubing to avoid contamination during manipulation after autoclaving.
3. Cover the sampling and dispensing nozzles with aluminium foil.
4. Autoclave the tubing in a sterilization bag in for 15 minutes at 121°C.

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Preventive Maintenance Operations User Maintenance

Note: The autoclave temperature must not exceed 121°C and the duration must not exceed
20 minutes.

Note: Never use a cleaning cycle.

Note: Never use a destruction cycle. After autoclaving, leave the tubing to cool down for two
hours in the closed autoclave to avoid distortion.

Note: When manipulating the tubing, take care not to contaminate it.

Note: Keep the tubing in the bag prior to use.

Preventive Maintenance Operations

It is recommended to perform a preventive maintenance at least once a year.

Call bioMérieux Technical Assistance or your local bioMérieux representative.

Replacing the UV Lamp

To ensure the germicide effect of the UV lamp, we recommend changing it every 400 hours of
use.
Make sure that the unit is switched off.
1. Remove the protecting cover and the shuttle to access the lamp.
2. Turn the little red stick downwards and remove the lamp.

3. Insert the new lamp (with the writing part on top side) and give the red stick a 90° turn
counterclockwise.

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A Appendix - Configuring the LINX 4900 Printer

To activate dish printing, select Configuring the APS One from the main menu (see
Managing the Operating Parameters). Once the LINX printer and the APS One® have been
configured, you can start a cycle and print on dishes.

Printing on 90 mm dishes

Machine configuration (F4)

Printing height Printing width Printing delay Inverted message

Normal 0 125 NO
Configuration message
Type of message 16 Quality
Size of characters Bold Length of message
5 FH No 2 lines of 20
characters
5 FH No 1st line: max 20
characters
Put 1 separation pixel
2nd line: max 20
characters
3rd line: max 20
characters

5 FH Yes 1st line: max 12 bold

No characters
2nd line: max 20
characters

16 FH No Max: 1 line of 11
characters

Printing on 55 mm dishes

Machine configuration (F4)

Printing height Printing width Printing delay Inverted message

Normal 0 135 NO
Configuration message
Type of message 16 Quality

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 Appendix - Configuring the LINX 4900 Printer

Machine configuration (F4)

Size of characters Bold Length of message

7 FH No 1 line of 11 characters
5 FH No 1st line: max 11
characters
Put 1 separation pixel
2nd line: max 11
characters
3rd line: max 11
characters

5 FH No 1st line: max 11 bold

characters
2nd line: max 11
characters

16 FH No Max: 1 line of 7
characters

Related Links
Managing the Operating Parameters

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B Appendix - Configuring the LINX CJ400 Printer

1. Log in with a supervisor account.

2. To set the message parameters, press and select Message Settings.

3. Select Advanced.

4. Modify all the options with these values:

Value APS ONE™ APS 320

Print width 0.360 mm 0.400 mm
Print delay 45.0 mm 150 mm
Print height 0 -10
Print count N/A N/A
Horizontal flip YES NO
Vertical flip NO YES
5. To set the line parameters, press to select Line Setting.

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 Appendix - Configuring the LINX CJ400 Printer

6. Select Advanced.
7. Modify all the options with these values:

Value APS ONE™ APS 320

Line 1 2
Trigger to printhead 5.0 mm 5.0 mm
distance
Speed mode Fixed speed Fixed speed
Line speed 0,500 m/s 0,250 m/s
Print trigger Leading edge primary Leading edge primary
Default message 2x7 Western fixed 2x7 Western fixed
Photocell type NPN NPN
Line direction Left to right Left to right
Printhead height 0 mm 0 mm
Encoder voltage 5V 5V
8. To create a message, press and select Message store.

9. Select Create a new message.

10. Touch the blue field Touch here to enter a new message text.
11. Select the kind of data you want to enter:

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 Appendix - Configuring the LINX CJ400 Printer

12. To enter a value you will change for each cycle (the batch number for example), create
a text field.
13. Press the Validation key.
14. Select Edit properties.
15. Activate the Prompt user option.
Each time you begin a new cycle, the printer will ask you to fill a new value.

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C Appendix - Troubleshooting

Error Messages

When using the APS ONE™ instrument some error messages may be displayed to indicate a
technical problem.
1. Press the B key to select Stop the alarm.

2. Press the A key to select Correct the error.

The cleaning screen appears.

Follow the cleaning steps:

3. Open the cover.
4. If required, remove dishes that may be blocked, and move the pistons and the shuttle
via the keypad.
Before closing the cover make sure that the shuttle is in the forward position.
Once the cover is closed, the APS ONE™ instrument initializes. The Error screen
reappears.
5. Press the D key to select Acknowledge this error.

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Error Messages and Recovery Procedures Appendix - Troubleshooting

When the error occured while dispensing, press the A key to select Resume, to carry
on the cycle.

Error Messages and Recovery Procedures

This section lists the possible error messages and general problems that could occur when
using the system.

Problem Resolution
Error Message / Problem Cause Resolution
The protecting cover was left 1. Close the cover, and press
opened. the D key to select Close
cover.
2. Press the D key to select
Acknowledge the error.

The shuttle may not be placed Make sure that it is positionned in

properly. the front position of the unit, and
restart the APS ONE™ instrument.
One piece of dish may block piston If the problem recurs, call
movements. bioMérieux Technical Assistance
or your local bioMérieux
representative.
A dish or a piece of dish may be 1. Check that the operating area
jammed. is cleared.
2. Restart the APS ONE™
instrument.
If the problem recurs, call
bioMérieux Technical Assistance
or your local bioMérieux
representative.

Symptoms
Symptom Cause Cause(s) and possible
remedie(s)
When switching on the unit, it does The cover was opened when the Close the cover and follow the
not initalize properly. unit was switched on. cleaning procedure to start
initialisation (see Cleaning the APS
ONE™ instrument).
If the problem recurs, call
bioMérieux Technical Assistance
or your local bioMérieux
representative.

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Symptoms Appendix - Troubleshooting

Symptom Cause Cause(s) and possible

When switching on, the unit does
not light up.
The unit takes about 30 seconds to 1. Switch off the unit.
initialize and display the main
screen.
remedie(s)
2. Switch it back on.
If the problem recurs, check the
fuse on the left side of the unit.
If the problem recurs, call
bioMérieux Technical Assistance
or your local bioMérieux
representative.

The UV lamp does not light up.
Check that the lamp is enabled
(see Configuring the APS ONE™
instrument). If so, it may be faulty.
Replace it by a new one (see
Replacing the UV lamp).
If the problem recurs, contact
bioMérieux Technical Assistance
or your local bioMérieux
representative.

A pump does not prime and does
not dispense.
Check the pump calibration (see
Adjusting the Pump).
Check that the tubing used is
adequate (see Starting a
Dispensing Cycle and List of
Accessories).
Remove the tubing and start
dispensing with the pump’s cover
opened.
If the pump’s rolls are turning, the
tubing that you are using may be
inappropriate.
If the pump’s rolls are not turning,
contact bioMérieux Technical
Assistance or your local
bioMérieux representative.

Dispensing is initiated but no dish
is filled.
Clean the dish sensor.
Check that you are using the
appropriate shuttle and it is placed
properly (see Technical Data and
Specifications).
Check that dishes have not been
placed down.

The plates are crashing.
Check the plates quality.
Check that the plates size is
correct.
Check the shuttle used.
Check that you are using the
appropriate shuttle and plate size
(see Plates). Contact bioMérieux
Technical Assistance or your local
distributor.

Related Links
Cleaning the APS ONE instrument
Replacing the UV Lamp
Adjusting the Pump
Starting a Dispensing Cycle
List of Accessories
Technical Data and Specifications
Plates

161150-1329 - G C-3 APS ONE™

Revision History
This section contains a summary of changes made to each released revision of this document
starting with part number:
Change type categories

N/A Not applicable (First publication)

Correction Correction of documentation anomalies
Technical change Addition, revision and/or removal of information related to the product
Administrative Implementation of non-technical changes noticeable to the user

Notes: • Minor typographical, grammar, and formatting changes are not

included in the revision history.
• Not all versions may be available in all languages.

Release Date Part Number Change Type Change Summary

2009-11 161150-1329 - A First publication NA
Modifications
2009-11 161150-1329 - B Technical change following first series
of APS One
Screenshot
2010-04 161150-1329 - C Technical change modifications and
addition of chapters

Modification of
screens, addition of
piston pause
duration, replacement
of blood addition by in
line addition, addition
2011-05 161150-1329 - D Technical change
of biplate mode,
modification of table
«options and
accessories»,
addition of pause
mode

Removal of EC
certification + addition
2012-07 161150-1329 - E Technical change
of FCC and Canadian
Industry certification
Regulatory
modifications
2013-07 161150-1329 - F Technical change
following the AES
bioMérieux merger
2020-09 161150-1329 - G Administrative Format change
 bioMérieux SA
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69280 Marcy-l’Etoile - France
Tel. 33 (0)4 78 87 20 00 Fax 33 (0)4 78 87 20 90

www.biomerieux.com