Determination of Quinine:

Apparatus Required
1. Fluorimeter
2. Cuvettes
3. Volumetric flask
4. Beakers
5. Funnel
6. Tissue paper

Chemicals Preparation:

1. Sulfuric acid (0.05 M):

Dilute 3.0 ml of concentrated sulfuric acid to 1 lit with distilled water in a 1 lit volumetric flask
2. Standard Quinine solution:
Dissolve 82.3 mg of quinine in 100 ml of 0.05 M sulphuric acid in a 100 ml volumetric flask.
Dilute 0.5 ml of the above stock solution to 500 ml with 0.05 M sulfuric acid. The resulting
solution contains 0.001 mg quinine per ml / 1 ppm

Procedure:

Prepare dilute solutions containing 0.0001mg/ml to 0.0007 mg/ml from the stock solution in a
50ml volumetric flask.

Measure the fluorescence intensity for all the solutions at 445 nm using highest concentrated
solution as standard for fluorimeter. Use LF2 or an equivalent primary filter (Max = 350 nm) and
gelatin as the secondary filter if using a simple fluorimeter. Plot the graph between fluorescence
Intensity Vs concentration.

Preparation of different concentration standard solutions as below.

Take above 1 ppm solution to 50 ml volumetric flask and make up to the mark with 0.05M
Sulfuric acid
01. 5 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0001 mg/ml
02. 10 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0002 mg/ml
03. 15 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0003 mg/ml
04. 20 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0004 mg/ml
05. 25 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0005 mg/ml
06. 30 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0006 mg/ml
07. 35 ml of 1ppm standard sol into 50 ml volumetric flask – 0.0007 mg/ml

Sample
Transfer the given sample solution in to a 50 ml standard flask and make up to the mark using
0.05 M sulphuric acid. Measure the fluorescence intensity of the solution then find out the
concentration of the sample using the calibration curve.