Title: Standard Preparation

Issue no.01 Page No. : 1 of 4

Issue Date : 01/04/2021

DETERMINATION OF METALS IN FOOD AND OTHER SAMPLES BY

INDUCTIVE COUPLED PLASMA OPTICAL EMISSION SPECTROSCOPY ( ICP-
OES )
Standard preparation:-
Prepare all serial dilutions of metal from 1000 ppm NIST Traceable metal stock
solution. For Ag conc. Above 2 ppm, use more than 10 ml 50 % HCL Per 100
ml.
Store diluted standard solution in acid cleaned fluorocarbon or pp bottles.
Quality control standard: prepare from reference sources with analyte conc.
Near 10 x instruments detection limit,2 ml 50 % HNO3,10 ML HCL, and water
to 100ml.
Mixed multi element standard preparation from intermediate std.
Working standard Preparation:- General Metals- analysis mode-Axial View
Elements Main Intermedia Volume made Volume to be
(Mode) stock in te stock in of intermediate taken from
ppm ppm stock in ml main stock in ml
Lithium (Li) 1000 75 50 ml 3.75
Aqueous
Iron (Fe) Aqueous 1000 12.5 0.625
Manganese (Mn) 1000 12.5 0.625
Aqueous
Chromium(Cr) 1000 12.5 0.625
Aqueous
Zinc(Zn) Aqueous 1000 12.5 0.625
Copper (Cu) 1000 12.5 0.625
Aqueous
Molybdenum(Mo) 1000 12.5 0.625
Aqueous
Barium (Ba) 1000 12.5 0.625
Aqueous
Cobalt( Co) 1000 12.5 0.625
Aqueous
Tin( Sn) Aqueous 1000 12.5 0.625

Aluminium (Al) 1000 7.5 0.375 Standar

Aqueous d
Nickel( Ni) 1000 2.5 1.25 mixture
Aqueous ( genera
Silver( Ag) 1000 2.5 1.25 l&
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 Title: Standard Preparation

Issue no.01 Page No. : 2 of 4

Issue Date : 01/04/2021

Aqueous toxic)
Cadmium(Cd) 1000 0.75 0.375
Aqueous
Lead (Pb) 1000 2.5 0.125
Aqueous
Arsenic( As) 1000 2.5 0.125
Aqueous
Selenium ( Se) 1000 2.5 0.125
Aqueous
Antimony (Sb) 100 2 0.625
Aqueous
Mercury ( Hg) 100 0.5 0.25
Aqueous
Standard Preparation:-
General metal mixture – Linearity in ppm
Vol. taken of std. 1 Std.2 Std.3 Std.4 Std.5 Std.6
intermediate stock to
make up 25 ml
0.05 ml 0.2 ml 1.0 ml 2.0 ml 3.0 ml 6.0 ml
Lithium ( Li) 0.15 0.600 3.0 6.0 9.0 18.0
Iron (Fe) 0.025 0.1 0.500 1 1.5 3.0
Manganese ( Mn) 0.025 0.1 0.500 1 1.5 3.0
Chromium ( Cr) 0.025 0.1 0.500 1 1.5 3.0
Zinc ( Zn) 0.025 0.1 0.500 1 1.5 3.0
Copper ( Cu) 0.025 0.1 0.500 1 1.5 3.0
Molybdenum(Mo) 0.025 0.1 0.500 1 1.5 3.0
Barium( Ba) 0.025 0.1 0.500 1 1.5 3.0
Cobalt( Co) 0.025 0.1 0.500 1 1.5 3.0
Tin ( Sn) 0.025 0.1 0.500 1 1.5 3.0
Aluminium ( Al) 0.015 0.060 0.300 0.600 0.900 1.800
Nickel (Ni) 0.005 0.020 0.100 0.200 0.300 0.600
Silver ( Ag) 0.005 0.020 0.100 0.200 0.300 0.600
Cadmium ( Cd) 0.0015 0.006 0.030 0.060 0.090 0.180
Lead ( Pb) 0.005 0.020 0.100 0.200 0.300 0.600
Arsenic ( As) 0.005 0.020 0.100 0.200 0.300 0.600
Selenium ( Se) 0.005 0.020 0.100 0.200 0.300 0.600
Antimony ( Sb) 0.004 0.016 0.080 0.160 0.250 0.500
Mercury ( Hg ) 0.0005 0.004 0.020 0.040 0.060 0.120
Sodium ( Na ) 0.25 1.0 5.0 10.0 15.0 30.0

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 Title: Standard Preparation

Issue no.01 Page No. : 3 of 4

Issue Date : 01/04/2021

Essential Metal Mixture – Linearity in PPM

Vol. taken of 0.25ml 0.5 ml 2.5 ml 5.0 ml 12.5 ml 22.5 ml
intermediate stock to
make up 25 ml
Phosphorous ( p ) 0.25 1.0 5.0 10.0 25.0 45.0
Potassium (K) 0.5 1.0 5.0 10.0 25.0 45.0
Sodium ( Na) 0.5 1.0 5.0 10.0 25.0 45.0
Calcium (Ca) 0.25 1.0 5.0 10.0 25.0 45.0
Magnesium (Mg) 0.25 1.0 5.0 10.0 25.0 45.0

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 Title: Standard Preparation

Issue no.01 Page No. : 4 of 4

Issue Date : 01/04/2021

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