Primer3 Output (primer3_results.cgi release 0.4.0) https://bioinfo.ut.ee/cgi-bin/primer3-0.4.0/primer3_results.

cgi

Primer3 Output

No mispriming library specified

Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 76 20 59.96 50.00 6.00 2.00 GAGGAATTCGCAACTGAAGC
RIGHT PRIMER 323 20 59.75 50.00 4.00 2.00 TCCGGGGTCAGATTGATAAC
SEQUENCE SIZE: 1485
INCLUDED REGION SIZE: 1485

PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

1 ATGGCTAACTATTTTAACACTCTGAATCTGCGCGAACAGCTGGATCAGCTGGGTCGTTGT

61 CGCTTCATGGCGCGCGAGGAATTCGCAACTGAAGCGGACTACCTGAAGGGTAAGAAAGTG
>>>>>>>>>>>>>>>>>>>>

121 GTCATCGTAGGCTGTGGCGCGCAGGGCCTGAACCAGGGTCTGAATATGCGTGATAGCGGC

181 CTGGACGTGTCTTACGCACTGCGTCAAGCGGCCATCGATGAACAGCGTCAGTCTTTCAAA

241 AATGCTAAGAACAACGGTTTCAATGTAGGTTCTTACGAACAGCTGATTCCGACGGCGGAC

301 CTGGTTATCAATCTGACCCCGGATAAACAGCACACCAGCGTGGTTAACGCGGTTATGCCG
<<<<<<<<<<<<<<<<<<<<

361 CTGATGAAACAGGGCGCTGCTCTGGGCTATAGCCATGGTTTTAACATCGTGGAAGAGGGC

421 ATGCAGATTCGTAAAGACATCACGGTAGTAATGGTGGCGCCGAAATGCCCAGGTACTGAG

481 GTTCGTGAGGAATACAAGCGTGGTTTTGGCGTTCCTACCCTGATTGCAGTGCACCCGGAA

541 AACGATCCGCAGGGCGAAGGTTGGGAAATCGCTAAGGCATGGGCTGCAGCCACTGGTGGC

601 CACCGCGCTGGTTGCCTGGCCTCTAGCTTCGTCGCAGAAGTAAAAAGCGACCTGATGGGC

661 GAACAGACCATCCTGTGTGGTATGCTGCAGGCCGGTAGCATCGTTTGCTACGAAAAGATG

721 GTTGCTGATGGTATCGACCCGGGCTACGCGGGCAAACTGCTGCAGTTTGGTTGGGAAACT

781 ATTACCGAGGCTCTGAAATTCGGTGGCATCACCCACATGATGGACCGTCTGAGCAACCCG

841 GCAAAGATCAAAGCATTCGAACTGTCTGAAGAGCTGAAAGACCTGATGCGTCCGCTGTAC

901 AACAAGCACATGGACGACATTATTTCCGGCCACTTTTCTTCTACCATGATGGCGGACTGG

1 dari 3 17/07/2023, 09.16

Primer3 Output (primer3_results.cgi release 0.4.0) https://bioinfo.ut.ee/cgi-bin/primer3-0.4.0/primer3_results.cgi

961 GCCAACGACGACAAGGATCTGTTCGGCTGGCGTGCGGAGACCGCTGAAACCGCATTTGAA

1021 AACTATCCGACCACTGACGTTAAGATCGCGGAGCAGGAATACTTTGACAACGGTATCCTG

1081 ATGATTGCAATGGTTCGCGCGGGCGTTGAACTGGCGTTTGAAGCGATGACCGCTTCTGGT

1141 ATCATCGATGAATCTGCATACTATGAGTCCCTGCACGAGCTGCCGCTGATCGCTAACACG

1201 GTGGCCCGCAAACGTCTGTATGAAATGAATGTCGTAATCTCTGACACGGCTGAATATGGT

1261 AATTATCTGTTCGCCAATGTGGCAGTTCCTCTGCTGCGTGAGAAATTCATGCCGAAAGTG

1321 GGTACCGATGTTATTGGTAAAGGTCTGGGTGTCGTTTCTAACCAGGTAGATAACGCGACT

1381 CTGATCGAAGTGAACTCCATCATCCGTAACCACCCGGTGGAATACATTGGTGAAGAACTG

1441 CGTGGTTATATGAAGGACATGAAGCGTATCGCCGTTGGTGATTAA

KEYS (in order of precedence):

>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 213 20 59.86 50.00 8.00 2.00 CATCGATGAACAGCGTCAGT

RIGHT PRIMER 372 20 59.83 45.00 4.00 2.00 CTGTTTCATCAGCGGCATAA
PRODUCT SIZE: 160, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

2 LEFT PRIMER 1276 20 59.87 50.00 4.00 1.00 AATGTGGCAGTTCCTCTGCT

RIGHT PRIMER 1472 20 60.24 50.00 4.00 0.00 GCGATACGCTTCATGTCCTT
PRODUCT SIZE: 197, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

3 LEFT PRIMER 308 20 60.27 45.00 4.00 1.00 TCAATCTGACCCCGGATAAA

RIGHT PRIMER 502 20 59.86 50.00 4.00 3.00 CACGCTTGTATTCCTCACGA
PRODUCT SIZE: 195, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

4 LEFT PRIMER 1276 20 59.87 50.00 4.00 1.00 AATGTGGCAGTTCCTCTGCT

RIGHT PRIMER 1444 20 60.30 50.00 3.00 3.00 CACGCAGTTCTTCACCAATG
PRODUCT SIZE: 169, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 11888 0 0 0 8 0 2097 7224 1 10 0 244 2304
Right 11943 0 0 0 8 0 2164 7056 0 6 0 239 2470
Pair Stats:
considered 724, unacceptable product size 702, high end compl 3, ok 19
primer3 release 1.1.4

2 dari 3 17/07/2023, 09.16

Primer3 Output (primer3_results.cgi release 0.4.0) https://bioinfo.ut.ee/cgi-bin/primer3-0.4.0/primer3_results.cgi

(primer3_results.cgi release 0.4.0)

3 dari 3 17/07/2023, 09.16