LEARNING ACTIVITY SHEET

QUARTER 2 – WEEK 6

Darlynne Mae B. Ducusin

12-Thomson
Marivic B. Ducusin
Sir Napoleon Arenas

Activity 6 OXIDATIVE PHOSPHORYLATION

Electron Transport System and Chemiosmosis
Objective: Distinguish major features of electron transport system and chemiosmosis
Part I Electron Transport System
The electron transport chain is the last component of aerobic respiration and is the only
part of glucose metabolism that uses atmospheric oxygen. Most of our energy is stored in the
form of reduce electron carrier NADH and FADH2. The electron transport chain is a process in
which NADH and FADH2 hands down electrons to a chain of carrier molecules. A series of
special acceptor molecules arrange in a precise sequence on the inner membrane of the
mitochondria. The energy electron pair is released at several points along the chain. And as the
electron pair goes from one electron carrier to another, oxidation – reduction reaction occurs
among the electron carrier. The electrons are passed along the chain until they’re given to
oxygen. Oxygen is known as the final electron acceptor, because it is the last molecule in the
electron transport chain to accept electrons.
Guide Question

1. Electron transport system occurs along the matrix inner membrane of the
mitochondria
2. One of the goals of electron transport is to reduce oxidize the electron carrier
back to “empty” state
3. The principal substance (substrate) that enters the electron transport system are
NADH
________________________ FADH2
and _______________________.
4. Complex I in the electron transport system is also known as
cytochrome oxidize complex NADH dehydrogenase complex
5. Complex I accept electrons from NADH and transferred them to
complex II ubiquinone
6. Complex I pump hydrogen ion across the membrane from the matrix of mitochondria to
inner mitochondrial membrane intermembrane space
7. Complex II directly receive electron from NADH FADH 2
8. Which electron acceptor receive electron from complex I and complex II
cytochrome ubiquinone
9. What is the final electron acceptor or last electron carrier found at the end of the
transport chain cytochrome ubiquinone oxygen
10. Which complex hold an oxygen molecule cytochrome oxidoreductase
cytochrome oxidase complex
11. Electron transport is aerobic anaerobic process
 Part II Chemiosmosis

As the electron flows in the electron transport chain part of the energy of
the electron is utilized to transport the hydrogen ions from the mitochondrial
matrix to the intermembrane space until the concentration of H+ increases in the
intermembrane space. A proton gradient or difference in the H+ ion concentration
is created and the ions flow back to the mitochondrial matrix by “diffusing” into
an ion channel having the enzyme ATP synthase. As the H + passes through the
channel, ATP synthase catalyzes the phosphorylation of ADP with inorganic
phosphate (Pi) forming ATP with the help of energy release by the electrons. This
mechanism of ATP production is by chemiosmosis.

Guide Questions
1. Explain why respiration is important in all living organism?
The process of releasing energy from meals is called respiration, and it occurs within the body’s cells. Respiration is crucial for life since it
_______________________________________________________________________
supplies energy for all of the biological processes that keep organisms alive.

2. What drives the phosphorylation of ATP?

To drive the synthesis of ATP, photo phosphorylation uses the radiant energy of the sun.
_________________________________
3. Make a concept map about metabolic pathway of cellular respiration (aerobic
respiration)?
2ATP Cytoplasm Aerobic Process
________________________________________________________________________

Cellular Glucose Glycolysis Kreb’s Cycle Pyruvate

Respiration 2FADH
2ATP
Anaerobic 6NADH
Process Mitochondria
 Electron
Fermentation Transport Chain

Lactic Acid 36 ATP

Part III Complete the table by supplying the necessary information

Stages Substrate/ Input Products/ Output

Glycolysis glucose, 2 ATP pyruvate, ATP, NADH

Pyruvate Dehydrogenase
Complex (PDC) pyruvate ATP

Krebs Cycle acetyl Co-A, NADH, CO2

acetyl Co-A
Electron Transport System
NADH, FADH2 NADH, FADH, ATP, CO2
(oxidative phosphorylation)

Choices:
NADH. FADH2 NADH, FADH, ATP, CO2
Glucose, 2ATP Pyruvate, ATP, NADH
Pyruvate ATP
Acetyl Co – A Acetyl Co – A, NADH, CO2
Part IV Identify the site or location where the following stages of cellular respiration occurs

LOCATION
Glycolysis cytoplasm
Pyruvate Dehydrogenase Complex (PDC) inner membrane of mitochondria
Krebs Cycle matrix of mitochondria
Electron Transport System (oxidative
phosphorylation) inner membrane of mitochondria

Prepared by: NAPOLEON Y. ARENAS

Master Teacher II