E.

coli Day 1 Day 2 Day 3 Day 4 Day 5

LB X 29 mm Not visible Not visible Not visible

due to due to due to
contaminat contaminat contaminat
ion ion ion

2X holiday No growth No growth No growth No growth

0.7% X 9 mm 0.8 cm has Not visible Not visible

maggots due to due to
contaminat contaminat
ion ion

2X holiday 17 mm 17 mm 18 mm 18 mm

0.5% X 10 mm 0.9 cm has Not visible Not visible

maggots due to due to
contaminat contaminat
ion ion

2X holiday 1.5 cm 1.5 cm 1.7 cm 2.0 cm

0.3% X 4 mm Not visible Not visible Not visible

due to due to due to
contaminat contaminat contaminat
ion ion ion

2X holiday Media Media Media Media

disoriented disoriented disoriented disoriented

S. aureus Day 1 Day 2 Day 3 Day 4 Day 5

LB X 4.5 mm x 4.5 mm 4.5 mm

2X holiday 5 mm 7 mm contaminat contaminat

ed ed

0.7% X 4.5 mm x 6 mm 7 mm

2X holiday 5 mm suspected maggots + maggots +

fungal contamina contamina
growth nts nts
0.5% X 4 mm x 6 mm 9 mm

2X holiday 10 mm 15 mm 15 mm maggots

0.3% X 5 mm x 12 mm disoriented

2X holiday there’s disoriented disoriented disoriented

growth but
point of
inoculation
cannot be
determined

Shigella sp. Day 1 Day 2 Day 3 Day 4 Day 5

LB X holiday 5mm maggots – –

2X holiday 9 mm 11 mm The The

presence presence
of maggots of maggots
and and
contamina contamina
nts nts
obscured obscured
the point of the point of
inoculation inoculation

X holiday 9mm maggots – –

0.7%
2X holiday 10 mm 6 mm The The
presence presence
Note: The of maggots of maggots
plate has and and
been contamina contamina
severely nts nts
contaminat obscured obscured
ed at this the point of the point of
time so the inoculation inoculation
diameter of
the growth
was
unclear.
We only
measured
until where
the circle
was
 opaque
white.

0.5% X holiday 15 mm maggots – –

2X holiday 13 mm 16 mm The The

presence presence
of maggots of maggots
and and
contamina contamina
nts nts
obscured obscured
the point of the point of
inoculation inoculation

0.3% X holiday Cannot be maggots – –

clearly
determined
; multiple
spread due
to agar
disintegrati
on

2X holiday 10 mm 15 mm The The

presence presence
of maggots of maggots
and and
contamina contamina
nts nts
obscured obscured
the point of the point of
inoculation inoculation

Salmonella sp. Day 1 Day 2 Day 3 Day 4 Day 5

LB X holiday 1 mm 2 mm maggots maggots

2X holiday Cannot be – – –
clearly de-
termined;
heavy
presence
of fungal
and
microbial
contami-
 nants
noted

0.7% X holiday 7 mm 8 mm maggots maggots

2X holiday maggots – – –

0.5% X holiday 9 mm 9 mm maggots maggots

2X holiday maggots – – –
and flies

0.3% X holiday 2 mm 3 mm maggots maggots

2X holiday maggots – – –

Congrats if you made it to this point! Go crammers!

Salamat poz