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    Bacillus anthracis is identified through a flowchart examining its major characteristics including its Gram stain morphology, colony morphology, catalase production, beta hemolysis, and motility. If the organism is Gram-positive rods, non-motile, non-hemolytic, and catalase positive, it is not ruled out as B. anthracis and the LRN Reference Level Laboratory should be contacted to further identify the suspected agent.

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    Bacillus anthracis is identified through a flowchart examining its major characteristics including its Gram stain morphology, colony morphology, catalase production, beta hemolysis, and motility. If the organism is Gram-positive rods, non-motile, non-hemolytic, and catalase positive, it is not ruled out as B. anthracis and the LRN Reference Level Laboratory should be contacted to further identify the suspected agent.

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    44 views1 page

    Anthrax Flowchart PDF 508 Branded

    Uploaded by

    gaber 230
    Bacillus anthracis is identified through a flowchart examining its major characteristics including its Gram stain morphology, colony morphology, catalase production, beta hemolysis, and motility. If the organism is Gram-positive rods, non-motile, non-hemolytic, and catalase positive, it is not ruled out as B. anthracis and the LRN Reference Level Laboratory should be contacted to further identify the suspected agent.

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    Bacillus anthracis

    Identification Flowchart

    Major characteristics of Bacillus anthracis:


    Gram stain morphology: Large, Gram-positive rods. Spores may be found in cultures grownin
    5% CO2 but not usually in clinical samples
    Colony morphology: Ground glass appearance, no hemolysis or pigment on BAP

    Perform all work in a biosafety cabinet using BSL-3 precautions.

    Yes
    Bacillus anthracis is ruled out.
    No Continue with routine
    Catalase positive?
    identification.
    Yes

    Bacillus anthracis is ruled out.


    Yes
    Beta hemolytic? Continue with routine
    identification.
    No

    Bacillus anthracis is ruled out.


    Yes
    Motile? Continue with routine
    identification.
    No

    Bacillus anthracis is not ruled out.


    Call the LRN Reference Level Laboratory and send suspected agent.

    Report: Possible Bacillus anthracis submitted to LRN Reference Laboratory.

    This job aid is a component of the free, on-demand CDC training course “Bacillus Anthracis.” Find the
    course at https://www.cdc.gov/labtraining.

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