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    This document summarizes several chemical methods for measuring analytes like glucose, lipids, non-protein nitrogen, bilirubin, and more. For glucose, methods include copper and ferric reduction reactions and a condensation reaction that produces a green color. Cholesterol measurement methods involve saponification, extraction, purification and color development reactions like Lieberman-Burchard that turns green. Triglyceride analysis uses a colorimetric method that originally turns blue but is modified to pink.

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    This document summarizes several chemical methods for measuring analytes like glucose, lipids, non-protein nitrogen, bilirubin, and more. For glucose, methods include copper and ferric reduction reactions and a condensation reaction that produces a green color. Cholesterol measurement methods involve saponification, extraction, purification and color development reactions like Lieberman-Burchard that turns green. Triglyceride analysis uses a colorimetric method that originally turns blue but is modified to pink.

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    21 views5 pages

    CC Colored Test Summary

    Uploaded by

    mendoxastar
    This document summarizes several chemical methods for measuring analytes like glucose, lipids, non-protein nitrogen, bilirubin, and more. For glucose, methods include copper and ferric reduction reactions and a condensation reaction that produces a green color. Cholesterol measurement methods involve saponification, extraction, purification and color development reactions like Lieberman-Burchard that turns green. Triglyceride analysis uses a colorimetric method that originally turns blue but is modified to pink.

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    of 5

    CARBOHYDRATES: GLUCOSE MEASUREMENT

    Chemical Methods
    A. Copper Reduction
    Folin-Wu Cu+ + Phosphomolybdic acid  Phosphomolybdenum Blue
    Nelson-Somogyi Cu+ + Arsenomolybdic acid  Arsenomolybdenum Blue
    Neocuproine Cu+ + Neocuproine  Cu- neocuproine complex Yellow - orange
    B. Ferric Reduction
    aka. Hagedorn- Jensen Ferric cyanide  ferrocyanide colorless
    (Yellow-orange)

    C. Condensation
    aka. Dubowski Reaction Glucose + o-toluidine  Schiff’s base Green
    LIPIDS: PLASMA LIPID ANALYSIS

    Analytical Methods
    A. Cholesterol
    Chemical Methods
     Saponification S- KOH
     Extraction E- Bloor’s Reagent
     Purification P- Digitonin
     Color development C- H2SO4 (Sulfuric Acid) and acetic acid

     Acetic Anhydride (Lieberman Burchard) Green

     Ferric Chloride (Salkowski Method) Red


    *Modified Abell-Kendal Method S- Alcoholic KOH
    E- N-hexane
    P- Digitonin Green
    C- Acetic Anhydride (Lieberman Burchard)
    B. Triglycerides
    Chemical Methods
    Triglyceride + H2O  glycerol + 3 fatty Acids
    1. Colorimetric Van Handel and Zilversmit Original - Blue
    Modified- Pink
    2. Fluorometric Hantzsch Yellow Lutidine
    Compound

    NON- PROTEIN NITROGEN

    Analytical Methods
    Chemical Methods
    Urea
    Chemical Method Diacetyl monoxime + H2O  diacetyl + Yellow
    aka. Fearon Method hydroxylamine
    Diacetyl monoxime method
    Direct Method Diacetyl + urea  Diazine
    Creatinine
    Chemical (Jaffe Method) Creatinine + alkaline picrate  red-orange tautomer Red-orange
    (Janovski Complex)

    *Don’t forget to study techniques to improve the specificity of this method

    Uric Acid
    Chemical
    (Phosphotungstic Acid Method)

     Caraway Method Blue (tungsten)


    (Henry, Caraway, Archibald) Uric acid +H3 PW12 O40 + O2  allontoin + tungsten +
    CO2
     Newton, Folin, Method
    Ammonia
    A. Potentiometric Measurement of pH change as NH3 diffuses through IDMS or Ammonia
    a selective membrane selective Electrode
    B. Colorimetric
     B 1.1 Indicator dye NH3 + bromphenol blue  blue dye Blue
     B 1.2 Nesslerization NH4++ K2 (HgI4)  dimercuric ammonium iodide Yellow  orange
     B 1.3 Berthelot NH4+ + NaOCI + phenol  indophenol Blue
    BILIRUBIN DETERMINATION
    A. Colorimetric-Spectrophotometric
    Evelyn-Malloy Jendrassik-Grof
    Reagents Diazo reaction (0.1% sulfanilic acid, Diazo reagent, Sodium acetate,
    0.5% sodium nitrite) ascorbic acid, alkaline titrate buffer
    Accelerator/ Solubilizer Methanol Caffeine/ Caffeine Sodium Benzoate
    pH of the reaction Acid Alkaline
    Color reaction Red-purple Blue
    Absorbance maximum 560 nm 600 nm
    B. Direct spectrophotometric 450 nm

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