lshni

Name A. Meha
Chass BSC (itnobiology)

Course Zhstpurer, Biotochno logg

TiHHe techrigues.

Cowrse Co de: BDMB tDS

Aol) odlboag
No
1Yo23
eq
 How will Pnepare Culdiore media fon The
aae

Cultvalon miC rwOngaUum

MiCrohlal Cullure media Preptralion a he

Pro Cess
Pro Ces miXing ubvents, agents pr beprung
and maintal ning he Osmolue balance as we
a ,

n dicalor Creale an
Aolecue ohibi tor on

nouth and
On broh hat 3pponts the Cullore
agan i C r o blal
miCroo rgarisms.
kayl the requdar
negudan
h e erertiabion roilre
ouire kaslk the
duo
med a
Preparalon

palhogerc mi(o bes n

me
psonito Appilage and

mi Ccbiolagicaldewng

LgLes_ _coltne meda

medè Bhould prouide
bual Cullwre
Micro all on specbe
qro
ut Cond lions en
opbimas Com 5/ 1ion
ovofarts he preciBe
res on The sp
on SPecies being cuedbrhe
ccies keing
deperdds
medin
a 7he medun PH reds
joas
ad Re oppbcalon
and
on 7he mi(ruoorganis
depending
be adjs sauo
t Culloe media
clasi/ied
mi(o6ial chemical onsbte
geuerral
,l e
pananelers

and hntlion yres m e dia

noliire
Dhyzical
Paramelers
descoibe d are
hese
ered by
lou
 Clanipication OF Cro bial Cullive meduá e
Chomical (omyps1ion

á media debred
Byheke mediun:
a

culwre pheroauds
Chenical Commo nal wed
bac1eria
and phetoly hele
7rephs Buck Cyane
sed n research t
Prohs wide
mela bo s n .
dudy milrsorgarusm
Con taining ron detres
Conpled medh is a re dua meat
Buch as Peplo re
Chemicad Cooponenb meel the
ecbracl7hal
and Yeas
ecbracl, drert
mulrooryary

regirenenls e with
ruubs'h ora nicro les
Cullire asidóy
s
Conplee eed
rutrilional reguirment

Naliwre
on physLcal
Culine mediá Based
o lo-3o7
agan a g a
kolbs e din ses7
g a s broh.Aold
Jolatr sokdg he
dierer mtre es
cullires, make
oher es tadish Pure
ench 3labs.
om
malle agar
gansland,
and
Conlain ang
7
bgoid medium does ro

culaton and
and
agers.Aer uno
Bob dfig Tke pern o a
betome vi sible
in cubalion te
mpd may On broT bhurrry
MiCAoe lad cultlwe me dià Base don
Preparalion meThod:

eady-l6- we meduun b a Aobid an gwd

gad
medin ppbed plats. lottlA.hbes on 0Ther
Conanens tn ready -o useorm o

e r renelting and Applemecirg

Mediun PrepredJrom omneriCall dehydrated

by orm which
formudalort 3 mediun
peguirek ehy dralion
and Prolesirg before se.

resubliny etler a conplete mediun on a

lomplele medium whic Ayplement are added

beporve se.
Me diu Prepaed rrom nd viduad

omponens a me diuum produced

a milno biobg dakoralog entinely rom

iodvdual igrelienls.
mede
Preparalon K miroblal Caldane

le dà Preparallen ro dehy brall

Commericalormmlalershould be peruned
marutac fwre n s ecko ef
ollewiag
le plonel Veast eebact
Aubstares. and and hola. 4 roaifed achtsue
Conss tency The meáiun. The negure ameut7
dehydratd ned um oy ind idua igred'enlt.
Qre ssolued i dshled uatir by Cen lirueud
Ainug 6lbued by Jenling edi
Canteinig
agar hould be adepual oabed h rere
ag:ahion sebre heaig he PH musi se aagai
an d ho mediuw 4 dspen3ed i 6 pprepria
Contarer r 8torilhzakin 4moiat heat i a
auo clae 4leelSers)e 3bsancel8

Cecddare
Cecl tawre m e da must be Ared
Petibe d enperalores Preuert moabfcauon
ohe (ompos7,fion 1and re onge han te
prrepaalèn and Aarage
Producl shesp-4e-Asepl
are ayseria fechon u a e r los on tonaje
be minimized by inpeun eable wtapiny and/an
Can be
Can
stonage a5 c sc chemtèal daguofLon g. shdakou
be elerdel 44
O7 an 7iitrobial
Aoss, Can
aetechon
cend ydrnbon
Pon Ligl. haal
tomw/en lapebcabont
e e n a n n l e s w u . 1gmaaldrick
microbial cuhn medo Pmeand4
milnobiologtcal etng -