Research question: How does the osmolarity of sucrose solution ranging from

0.0M to 0.8M affect the change of weight and length of the tissue of Solanum
tuberosum?
1. Introduction

2. Investigation

Variables and Justification of Variables

Independent Variable: Osmolarity of sucrose solutions: 0.0M, 0.2M, 0.4M, 0.6M,

0.8M

 The independent variable is altered by soaking the Solanum tuberosum

tissue in sucrose solutions of 0.0M, 0.2M, 0.4M, 0.6M, 0.8M for 1 hour.
This variable was chosen to test the osmolarity of the tissue by simulating
a hypotonic, hypertonic and isotonic environment.

Dependent Variables: Length and weight of Solanum tuberosum sample

 The Length and weight of the Solanum tuberosum samples reflect the
amount of sucrose that was ingested in the process of soaking by osmosis.
Samples that are larger and heavier have ingested more sucrose, thus
meaning the environment is better suited for osmosis.

Controls

Volume of sucrose solutions

 All the sucrose solutions were of the same volume, only differing in the
sucrose concentration.

Time spent soaking

 All the samples were left to soak for 1 hour and put in the solution
simultaneously and taken out of it simultaneously.

Number of samples in each batch

  Each batch had 5 samples, that were left to soak in each of the solutions to
ensure fair calculations for each batch.

Temperature

 All the solutions and samples were in the same room, and the temperature
was the same throughout. This was to ensure identical conditions that
might affect osmosis.

Pressure

 All the solutions and samples were in the same room, and the pressure
was the same throughout. This was to ensure identical conditions that
might affect osmosis.

Solvent

 All the solutions were made with distilled water, so any impurities wouldn’t
affect the process of osmosis.

Type of sample

 All the samples were of the same species of Solanum tuberosum, having
the same features and osmolarity.

3. Procedure

3.1 Materials

 5 50mL glass beakers

 1 analytical balance (±0.001g)
 Spoon
 5 50 mL volumetric flasks (±0.05mL)
 Handful of potatoes
 Knife
  Ruler (±0.5mm)
 Drilling tool
 5 glass rods
 Paper towels
 Weighing dish

3.2 Methodology

3.3 Ethical, Safety and Environmental Concerns.

In this experiment we were working with household materials that were safe for
human consumption, and the only concerns were ethical concerns pertaining to
the wasting of potatoes, why we needed do be careful as to waste as little as
possible. No risk during disposal or handling of materials.

4. Raw Data

5. Processed data

6. Evaluation