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  • Protein Problems

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    saumya sharma
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    Proteins can be produced through recombinant DNA techniques by determining the amino acid sequence of the target protein, transcribing its mRNA, cloning the cDNA, and introducing the vector into a suitable host cell. The foreign protein is then expressed and can be purified. There are many industrial applications of enzymes, such as using chymosin to coagulate milk into cheese or using cellulases to process cotton. The 20 standard amino acids are the building blocks of proteins and come together to form complex three-dimensional structures through interactions between their R groups.

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    Proteins can be produced through recombinant DNA techniques by determining the amino acid sequence of the target protein, transcribing its mRNA, cloning the cDNA, and introducing the vector into a suitable host cell. The foreign protein is then expressed and can be purified. There are many industrial applications of enzymes, such as using chymosin to coagulate milk into cheese or using cellulases to process cotton. The 20 standard amino acids are the building blocks of proteins and come together to form complex three-dimensional structures through interactions between their R groups.

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    7 views8 pages

    Protein Problems

    Uploaded by

    saumya sharma
    Proteins can be produced through recombinant DNA techniques by determining the amino acid sequence of the target protein, transcribing its mRNA, cloning the cDNA, and introducing the vector into a suitable host cell. The foreign protein is then expressed and can be purified. There are many industrial applications of enzymes, such as using chymosin to coagulate milk into cheese or using cellulases to process cotton. The 20 standard amino acids are the building blocks of proteins and come together to form complex three-dimensional structures through interactions between their R groups.

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    © All Rights Reserved
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    of 8

    PROTEINS

    # CHeese
    Se produclion:

    Tsolaien eh prochyoin Detuminajien


    aminoaád sebuence
    mlNA om at
    cells

    Tronsuptien: Biochemical synns


    mRNA CDNA hodyinosin
    DNA

    cloning o CDNA
    aajiate vecton
    Intoduce vecter nt suitable cg anusm

    Cenueusie) o< fochymosin ito active chuymosin


    at iow pt

    putatn 4 chymosin
    omucial iotua ti en
    Susface
    Chymosin glycopepde Aonucud
    remeved Caesin þeutides

    Aggnegatad calcium
    paracaeinate padide in
    milk (oalaen
    #Some enaymes 4 Dndusbial Appicalons
    Stanch
    Angaes
    Proteases proteins
    ubids
    pectinaues engymes in fuuit juicelpa
    Jactases diggt milk

    poduce am animal
    Celulaes Jhami celludaw)
    cenuels

    ’ feri cillin acyae - heduce penicillin


    #Shudual pretein sargment
    Pimay (chain o amuno acid)

    Stcenda (nked by useak H-bendo)


    Sheot

    Tetiay foduci ng une 3D shctue)


    (meu than eye auno aid chan ecui

    (Hae moqiebin) in firal 3D-shoupe )


    t AMINO ACIDS

    ’20 amimo acics


    COO

    ’Preltine , Cuclic amrino acd , excegion to this


    Alanine (ala ) Sevne (Se)

    Asgmine CAng Threoine (Th


    Asporagne (Asn) Tryprephan (Trp)
    Aspadicaid (Asp) Tyroche (iyr)
    Aspragjine |Atprnlicacid (As x) valine va)
    ysteine (y)
    qutaine (gn
    Cltamc acid (Gu)
    Gutomi ne |guatanie cad (gu)
    Hididing (HiS)
    Isoluwcine (Ile)
    Leucin (Leu)

    Methienine (Met)
    Phenyhalanine (Phe)
    Prci ne (ro)
    x tlon polar Ai;hrlc ()
    nlanune )

    Gey Ala Pro Val eu Jnlou

    * folar (5).
    Ser -The -Cys - Aup -yn
    * Anomatic (3)

    *ve chaaedi(3) Lyy -bg His


    (2):
    chagd
    q ng- acid.

    - lon ct wain molecue bebtde bend


    (cendmuabe)
    ’ N teumin: beginning chain
    Ctaminal : end chain

    tPxaten uiticaien:

    bo Succesil, SA

    eat tham bakene.


    (wgmi) Totu Toto Fold
    Velume Dctem Acvitt metein
    Lenc
    acuvity pust Vield
    |Yield.

    AB, B, xv, A Bi-X Alxo


    Ai

    Ba B,xvya P, B
    Au

    B3 X3 A3
    XA

    Adivity xPrtteim Velume


    Tetad faein tenc Activity xVetumg Pctein

    Shucitie acivit Rdivity


    Pastain
    Fold uhicuen- SA,

    (Total actiity)n
    (Totad ativihy)
    min
    Ameunt
    engye subyate
    2r/30C and

    SI unit
    katal (Lat)
    acti vity is dofned
    paramolos

    BA, Fold puilcatien, yjld


    bases
    #Amine acids can cct as Acidls ¢
    Lduadnaliie : amghey
    vehch the nol eleco( chage
    charateiic pt al

    Fel gyeiene,has he
    ferisable goup in t
    sico choçM.
    I= 1(k, pBe)

    # pephde bond Ne oj aine acids

    Remeves eny amYo temnal eside mem a


    peptido
    pegtide : React
    ’ Amuno teimnol pesidue emened cu plenfthio
    hydantsin doi vative
    unut entire geul
    is ditined
    ey sUnsitve

    +heps in gencing a ieigtegid


    (G)
    mthec: Tdenti he aNno teminal
    Song eide
    Edman doati on - e eals otine Squene
    cell s
    Solsent
    Stitte Wows tond

    () Tscto nic

    () hupcten

    C: qtamal+

    C but
    Ond y-Pro
    yngntbemide

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