Lipid Profile Estimation

Methods and Importance

Dr. Mina Hemmati

Associate Professor in Clinical Biochemistry
‫ﻟﯿﭙﯿﺪﻫﺎي ﺑﯿﻮﻟﻮژﯾﮑﯽ ﮔﺮوه ﻣﺘﻨﻮﻋﯽ از ﺗﺮﮐﯿﺒﺎت ﺷﯿﻤﯿﺎﯾﯽ ﻫﺴﺘﻨﺪ ﮐﻪ وﯾﮋﮔﯽ اﺻﻠﯽ و ﺗﻌﺮﯾﻒ‬
‫ﺷﺪه آﻧﻬﺎ ﻋﺪم ﺣﻼﻟﯿﺖ در آب اﺳﺖ‪.‬‬

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‫)‪Free Cholesterol (FC‬‬ ‫)‪Esterified Cholesterol (EC‬‬
‫ﮐﻠﺳﺗرول آزاد‬ ‫ﮐﻠﺳﺗرول اﺳﺗرﯾﻔﯾﮫ‬

‫ﮐرﺑن‬
‫ﺷﻣﺎره ‪3‬‬

‫اﺳﯾد ﭼرب‬
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‫‪ -‬از ﻧﻤﻮﻧﻪ ﺳﺮم ﯾﺎ ﭘﻼﺳﻤﺎ اﺳﺘﻔﺎده ﻣﯽ ﺷﻮد و ‪ 8-14‬ﺳﺎﻋﺖ ﻧﺎﺷﺘﺎﯾﯽ ﻻزم دارد‪.‬‬

‫‪ -‬روش اﻧﺪازه ﮔﯿﺮي ‪:‬‬

‫آﻧﺰﯾﻤﯽ و ﮐﺎﻟﺮﯾﻤﺘﺮي )رﻧﮓ ﺳﻨﺠﯽ(‬

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 ...‫اﺳﺎس آزﻣﺎﯾﺶ‬

ChE
Cholesterol ester + H2O Cholesterol + Fatty acid

ChE : Cholesterol Esterase

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 ...‫اﺳﺎس آزﻣﺎﯾﺶ‬

ChOX
Cholesterol + O2 Cholesterol-3-one + H2O2

ChOX : Cholesterol Oxidase

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 ‫اﺳﺎس آزﻣﺎﯾﺶ‬

PO
2H2O2+4-Aminoantipyrine + Phenol Quinoneimine + 4H2O

2 H2O2 + + + 4H2O

PO: Peroxidase

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 ‫روش ﮐﺎر‬

‫آب ﻣﻘﻄﺮ‬ ‫اﺳﺘﺎﻧﺪارد‬ ‫ﺗﺴﺖ‬ ‫ﻣﻌﺮف‬

Blank - -
* *
Standard - -
* *
Test - -
* *

C: Concentration
Ct = (OD t / OD s) x Cs OD : Optical Density
S: Standard
T : Test
λ = 546 nm
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