SCDS exacerbates many of the data science issues arising in bulk sequencing,

but it also constitutes a set of new, unique challenges for the SCDS community
to tackle. Limited amounts of material available per cell lead to high levels of
uncertainty about observations. When amplification is used to generate more
material, technical noise is added to the resulting data. Further, any increase
in resolution results in another—rapidly growing—dimension in data matrices,
calling for scalable data analysis models and methods. Finally, no matter how
varied the challenges are—by research goal, tissue analyzed, experimental
setup, or just by whether DNA or RNA is sequenced—they are all rooted in
data science, i.e., are computational or statistical in nature. Here, we propose
the data science challenges that we believe to be among the most relevant for
bringing SCDS forward.