Professional Documents
Culture Documents
B 4 Revision Pack
B 4 Revision Pack
Figure 1
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(1)
A student investigated the effect of different colours of light on the growth of tomato plant
seedlings.
Figure 2
Page 1 of 64
(b) Give two control variables the student should have used in their investigation.
1 _________________________________________________________________
2 _________________________________________________________________
(2)
Mean height of
Colour of light
seedlings in cm
Blue 14.5
Red 12.0
White 11.8
1 _________________________________________________________________
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2 _________________________________________________________________
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(2)
Another student investigated the effect of light intensity on the rate of photosynthesis.
The student counted the number of bubbles of oxygen produced by pondweed at different
light intensities.
Figure 3
(d) Describe how the student could change the light intensity.
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Page 2 of 64
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(2)
How could the student change the investigation to increase the number of bubbles
produced?
(1)
(1)
Figure 4
Page 3 of 64
Describe the pattern in Figure 4.
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(2)
(Total 11 marks)
Q2.
Algal cells can photosynthesise.
Figure 1
Page 4 of 64
(a) Describe the relationship between light intensity and distance, as the distance of the
algal cells from the lamp is doubled.
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(3)
The student recorded the pH of the water at the start and after one hour in each beaker.
pH of water
Distance of algal cells pH of water
after one
from lamp in cm at start
hour
20 7.8 9.1
40 7.8 8.8
60 7.8 8.5
80 7.8 8.3
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Page 5 of 64
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(4)
Corals:
• cannot move from one place to another place
• feed on tiny animals that float past in the water
• have algal cells living inside their own cells.
Figure 2
(d) Explain one way the algal cells use glucose for growth.
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(2)
(e) Suggest one advantage to the algal cells of living inside the coral cells.
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(1)
Page 6 of 64
(f) An increase in water temperature can cause coral to release the algal cells.
Suggest why coral may not survive without the algal cells.
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(1)
Scientists investigated how the concentration of oxygen in the water around one coral
changed during the day.
Figure 3
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(3)
(h) At C the rate at which oxygen is being produced equals the rate at which oxygen is
being used.
Page 7 of 64
Give the reason why.
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(1)
(Total 18 marks)
Q3.
This question is about photosynthesis and food production.
(a) How can oxygen production be used to show the rate of photosynthesis?
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(1)
The tomato plants were growing in a commercial greenhouse in the UK during winter.
(b) Name the factor that is limiting the rate of photosynthesis at point X.
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(1)
Farmers growing tomatoes commercially try to control the rate of photosynthesis and
Page 8 of 64
make maximum profit.
A farmer can control the temperature and carbon dioxide concentration in a greenhouse.
(c) What is the minimum light intensity a farmer should use to get the maximum rate of
photosynthesis shown in above graph?
(d) The light intensity you gave in part (c) may not give the farmer maximum profit.
Explain why.
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(3)
(e) Explain the results when the light intensity was 0 lux.
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(4)
(Total 10 marks)
Q4.
Plants absorb light to photosynthesise.
(1)
Page 9 of 64
The diagram below shows some of the equipment used to measure the rate of
photosynthesis.
(b) Describe a method to investigate the effect of light intensity on the rate of
photosynthesis.
Use the equipment in the diagram above and other laboratory equipment.
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(6)
The table below shows the number of extra algal cells after two days.
Page 10 of 64
lux days
0 no extra cells
Calculate the total number of algal cells after two days when the light intensity was
500 lux
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(d) Plot the data from the table above on the graph below.
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(3)
1. _________________________________________________________________
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2. _________________________________________________________________
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(2)
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(2)
(Total 16 marks)
Q5.
Page 12 of 64
A student used a potometer to investigate the rate of water uptake in a plant shoot.
Figure 1
The rate of water uptake is the distance the air bubble moves in a given time.
Table 1
Investigation Conditions
A Moist air at 25 °C
B Dry air at 15 °C
C Dry air at 25 °C
(a) After investigation A the air bubble had moved part way along the capillary tube.
Suggest how the student moved the air bubble back to 0 mm for the start of
investigation B.
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Page 13 of 64
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(1)
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(2)
Figure 2
(c) The cross-sectional area of the capillary tube was 0.8 mm2
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Page 14 of 64
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Table 2
0 0
1 6
2 16
3 22
4 30
5 42
You should:
Draw a line on Figure 2 to show the results you would expect for investigation C.
The air bubble would not move if the investigations were done in the dark.
Explain why.
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(3)
(Total 13 marks)
Q6.
Figure 1 shows a root hair viewed using a microscope.
Figure 1
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Describe how to use this microscope to observe a prepared slide of root hair cells at
a magnification of ×50
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Page 16 of 64
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(4)
Root hair cells absorb water and mineral ions from the soil.
Figure 2
The scientist determined the mass of nitrate ions absorbed by each seedling every 30
minutes for 4 hours.
0 0 0
0.5 100 60
1.0 145 95
Page 17 of 64
3.5 250 130
(c) Describe the changes in the rate of absorption of nitrate ions for the seedling with no
oxygen added.
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(3)
(d) Explain what the results in the table above show about how nitrate ions are
absorbed.
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(4)
Describe how nitrate ions are used in a plant to help the plant grow.
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(3)
(Total 18 marks)
Q7.
This question is about photosynthesis.
(1)
Aerobic
Endothermic
Exothermic
Oxidation
(1)
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Sodium hydrogencarbonate solution releases carbon dioxide gas for the pondweed.
(c) Counting the number of bubbles produced in 1 minute is not an accurate way to
measure the rate of photosynthesis.
Suggest two ways the method could be improved to measure the rate of
photosynthesis more accurately.
1. _________________________________________________________________
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2. _________________________________________________________________
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(2)
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(2)
(e) Light intensity can be calculated using the inverse square law:
Page 20 of 64
Where I is light intensity and d is the distance of the pondweed from the light source.
The student placed the pondweed at 5, 10 and 20 cm from the light source.
Explain how light intensity changes as the distance of the pondweed from the light
source is doubled.
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(3)
Distance of the
Number of bubbles
pondweed from the light
produced in 1 minute
source in cm
5 129
10 31
20 8
Predict how many bubbles of gas would be produced in 1 minute if the pondweed
was placed 40 cm from the light source.
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(2)
(g) Describe how the student could change the method to investigate the effect of
carbon dioxide concentration on the rate of photosynthesis.
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• two control variables.
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(3)
(Total 14 marks)
Q8.
Plants make glucose by photosynthesis.
(b) What is the name of the chemical that makes a leaf look green?
Cellulose
Chlorophyll
Chloroplast
Chromosome
(1)
(c) A test for starch is used to show that a plant has photosynthesised.
How does the presence of starch show that photosynthesis has taken place?
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(1)
She used a leaf that was part green and part white as shown in the diagram.
Page 22 of 64
This is the method used.
4. Spread the leaf on a white tile and test with iodine solution.
Reason: stains any starch.
(d) If the chemical reactions in the leaf were not stopped, the amount of starch in the
leaf would decrease.
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(1)
(e) Suggest why it is important to remove the green colour from the leaf before adding
iodine solution.
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(1)
The student wore safety goggles when testing the leaf for starch.
Give one other safety precaution the student should have taken.
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(1)
Page 23 of 64
What colour would part A and part B stain with iodine solution after the starch test?
A ____________________
B ____________________
(2)
(Total 8 marks)
Q9.
Students investigated the effect of light intensity on the rate of photosynthesis in
pondweed.
(a) The students could not make a firm conclusion because their method did not control
enough variables.
Give two variables the students have not controlled that would affect the rate of
photosynthesis.
1. _________________________________________________________________
2. _________________________________________________________________
(2)
50 5 8 6 5 6
40 10 8 9 4 9
Page 24 of 64
30 12 12 15 17 14
20 25 17 23 24 24
10 22 34 31 31 X
(b) Calculate the mean rate of bubbles produced per minute when the lamp was 10 cm
from the pondweed.
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(c) The mean number of bubbles released when the lamp was 30 cm away from the
plant was greater than when the lamp was 50 cm away.
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(d) The students wanted to find out if different wavelengths of light affect the number of
bubbles released.
Describe how the method could be adapted to find the effect of different
wavelengths of light.
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(2)
(Total 8 marks)
Q10.
This question is about breathing and gas exchange.
Page 25 of 64
Peak flow is how quickly air can be breathed out of the lungs.
The table below shows the peak flow of a person on two different days.
(a) The person has different peak flow results on Day 1 and Day 2.
Suggest one reason why peak flow was lower on the second day.
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(1)
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Page 26 of 64
There are many alveoli in the lungs.
Explain how two other adaptations of the alveoli allow efficient gas exchange.
1. _________________________________________________________________
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2. _________________________________________________________________
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(4)
(Total 8 marks)
Q11.
Fermentation in yeast is used in the manufacture of bread and alcoholic drinks.
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Page 27 of 64
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(1)
(b) Suggest why a layer of oil was needed on the surface of the mixture.
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(1)
(c) Suggest why the mixture was left for 20 minutes before the gas syringe was
attached.
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(1)
The volume of gas collected was recorded every 5 minutes for 45 minutes.
Table 1 shows the results for both flasks for the first 30 minutes.
Table 2 shows the results for the last 15 minutes, when both flasks were at 35 °C.
Table 1
Page 28 of 64
Flask at 2 °C Flask at 35 °C
0 0 0
5 0 26
10 0 52
15 0 78
20 0 98
25 0 108
30 0 115
Table 2
35 2 120
40 7 123
45 22 124
(d) Explain the results from 0 minutes to 45 minutes for the flask that was at 2 °C and
was then moved to 35 °C.
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(3)
(e) Explain the results from 0 minutes to 45 minutes for the flask kept at 35 °C.
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(4)
(Total 10 marks)
Q12.
Two students investigated the effect of exercise on breathing rate.
Breathing rate was measured by counting the number of times a student breathed in
during 1 minute.
Suggest two factors that could cause the students’ breathing rates at rest to be
different.
1 _________________________________________________________________
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2 _________________________________________________________________
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(2)
(b) Suggest one reason why the measurements for breathing rate may not be accurate.
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(1)
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(c) What time did the students start exercising?
(d) Describe two differences between the results of student X and of student Y.
1 _________________________________________________________________
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2 _________________________________________________________________
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(2)
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To increase the uptake of oxygen from the air
(1)
(f) The breathing rate of the students stayed high after the exercise stopped.
Why does breathing rate stay high after exercise has stopped?
(1)
(g) Give one other change that happens in the body during exercise.
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(1)
(Total 9 marks)
Q13.
A student investigated the effect of pond organisms on the amount of carbon dioxide in
their surroundings.
The student set up six boiling tubes as shown in the figure below.
• If the amount of carbon dioxide in the water increased the indicator turned yellow.
• If the amount of carbon dioxide in the water decreased the indicator turned purple.
Page 32 of 64
(a) What is the purpose of boiling tube A?
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(2)
(b) In which boiling tube would the indicator be the most yellow after 2 days?
Explanation _________________________________________________________
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(3)
(c) The colour of the indicator in boiling tube C had not changed after 2 days.
Suggest why.
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(1)
(Total 6 marks)
Q14.
A student investigates the rate of respiration in maggots.
Figure 1
Page 33 of 64
(a) Why does the student put the maggots on gauze?
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(1)
(b) When maggots respire they take in a gas from the air and release a different gas.
At the start of the investigation the student records the distance of the water droplet
from the bend in the capillary tube.
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(4)
(c) The table below shows the results the student calculated.
Rate of
Temperature in
respiration in
°C
units
Page 34 of 64
5 2.2
10 3.5
20 7.5
30 8.4
40 14.0
Figure 2
(1)
(d) How could the student find out if the result at 30 °C is anomalous?
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(1)
(e) Suggest what the value at 30 °C should be to fit the pattern of the graph.
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(1)
(Total 8 marks)
Q15.
An athlete ran as fast as he could until he was exhausted.
(a) Figure 1 shows the concentrations of glucose and of lactic acid in the athlete’s
Page 35 of 64
blood at the start and at the end of the run.
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(1)
(ii) Give evidence from Figure 1 that the athlete respired anaerobically during the
run.
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(1)
(b) Figure 2 shows the effect of running on the rate of blood flow through the athlete’s
muscles.
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(i) For how many minutes did the athlete run?
(ii) Describe what happens to the rate of blood flow through the athlete’s muscles
during the run.
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(2)
(iii) Explain how the change in blood flow to the athlete’s muscles helps him to
run.
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Page 37 of 64
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(4)
(Total 9 marks)
Q16.
(a) Complete the equation for photosynthesis.
light
energy
____________ + ____________ ____________ + oxygen
(2)
The scientists used the rate of oxygen production by the leaf discs to show the rate
of photosynthesis.
(i) The leaf discs did not produce any oxygen in the dark.
Why?
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(1)
Explain why.
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(2)
Page 38 of 64
(c) In their investigation, the scientists measured the rate of oxygen release by the leaf
discs in the light. The scientists then measured the rate of oxygen uptake by the leaf
discs in the dark.
Use the information from the graph to answer each of the following questions.
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(2)
(ii) Explain the effect of temperature on oxygen production in the light when the
temperature is increased:
from 25 °C to 35 °C
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Page 39 of 64
from 40 °C to 50 °C.
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(2)
(d) A farmer in the UK wants to grow orange trees in a greenhouse. He wants to sell the
oranges he produces at a local market.
He decides to heat the greenhouse to 35 °C.
Explain why he should not heat the greenhouse to a temperature higher than 35 °C.
Use information from the graph in your answer.
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(3)
(Total 12 marks)
Q17.
One type of training exercise involves alternating periods of walking and running.
The graph shows how an athlete’s heart rate changed during one 30-minute training
session.
Page 40 of 64
(a) (i) The athlete ran 6 times during the 30-minute training session.
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(1)
(ii) Immediately after the final run, the athlete rested for a short time before he
started to walk again.
____________________ minutes
(1)
Explain, as fully as you can, why this increase in heart rate is necessary.
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Page 41 of 64
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(4)
(Total 6 marks)
Q18.
Lactic acid production during exercise affects an athlete’s performance.
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(Total 2 marks)
Q19.
Many people who are overweight try slimming programmes.
Scientists selected a group of 40 people for each slimming programme and a control
group.
Each of the five groups was matched for age, gender and mass.
Page 42 of 64
Adapted from British Medical Journal, 2006, volume 332, pages 1309 –1314.
(a) Give two control variables that were used in this study.
1. _________________________________________________________________
2. _________________________________________________________________
(2)
(b) Give two conclusions that can be drawn from the results of this study.
1. _________________________________________________________________
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2. _________________________________________________________________
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(2)
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(2)
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(2)
(Total 8 marks)
Page 44 of 64
Mark schemes
Q1.
(a) any one from:
• root
• stem
1
• temperature
• number of leaves
• light intensity
ignore light unqualified
allow brightness / power of light / bulb
• type of soil
ignore nutrients
2
• there was very little difference in height of seedlings grown under white
and red light
allow seedlings grow taller under red light than
white light
2
Page 45 of 64
(and) move closer and / or further away (from pondweed)
allow place lamp / light different distances (from
pondweed)
allow use different power ratings of bulb / lamp
1
(f)
1
• after 35 / 300 (arbitrary units) the rate of photosynthesis then stays the
same / constant
do not allow photosynthesis stops
• the rate increases at a decreasing rate (between 100 and 300 arbitrary
units)
allow the rate increases at a decreasing rate
between 100 and 300 arbitrary units for 2 marks
if no other mark awarded allow photosynthesis
increases then levels out for 1 mark
2
[11]
Q2.
(a) (at 20 cm)
light intensity ∝ 0.0025
or
2.5 × 10‒3
1
(at 40 cm)
light intensity ∝ 0.000625
or
6.25 ×10‒4
1
Page 46 of 64
(because algal cells) used carbon dioxide (from the solution) for
photosynthesis
1
or
or
Page 47 of 64
(g) decrease in light intensity
allow no light
allow decrease in light
1
Q3.
(a) measure the volume of oxygen produced in a given time
or
when more oxygen is produced in a given time the rate of photosynthesis is faster
a reference to rate is needed
allow gas for oxygen
allow when oxygen is produced faster the rate of
photosynthesis is faster
ignore the faster the rate of photosynthesis, the
more oxygen is produced
allow the slower the oxygen is produced the
slower the rate of photosynthesis
or
less oxygen being produced in a given time, the
slower the rate of photosynthesis
1
(therefore) the additional costs might exceed the (additional) sale price / profit
or
(additional) costs could not be recovered by increasing the sale price of the
Page 48 of 64
tomatoes
ignore these additional costs would reduce profits
unqualified
1
Q4.
(a) carbon dioxide (+ water) oxygen (+ glucose)
both gases needed for the mark
allow correct formulae
words take precedence
1
(b) Level 3: The method would lead to the production of a valid outcome. The key
steps are identified and logically sequenced.
5−6
Level 2: The method would not necessarily lead to a valid outcome. Most steps are
identified, but the method is not fully logically sequenced.
3−4
Level 1: The method would not lead to a valid outcome. Some relevant steps are
identified, but links are not made clear.
1−2
No relevant content
0
Indicative content
• measure the distance between the pondweed and the light source
• count the number of bubbles or measure the volume of oxygen / gas
produced
• (measure oxygen / gas produced) in a set period of time
• change the distance between the light source and the pondweed or use
a different power lamp
• control colour of light
• control temperature using a heat screen / water bath
• use the same pondweed
• use the same length / size of pondweed
• control carbon dioxide supply
• idea of allowing time for pondweed to equilibrate
• repeat each test two or more times
• calculate a mean
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For Level 3 the method described must include;
• how the light intensity is changed
• the measurements needed to determine the rate of photosynthesis
• at least one control variable
or
(e) increasing light intensity increases the number of (extra algal) cells
do not accept as number of cells increases, light
intensity increases
1
the increase in the number of (extra algal) cells plateaus at 1 250 lux
allow the number of cells does not increase
above 1 250 lux
allow the number of cells is the same at 1 250
and 1 500 Lux
or
the increase in the number of (extra algal) cells plateaus at 2.50 × 106 (extra algal)
cells
allow the number of (extra algal) cells does not
increase above 2.50 x106
1
Q5.
Page 50 of 64
(a) opened and closed the tap (so water enters from reservoir)
allow opened the tap (carefully / gently)
1
(c)
an answer of 10.56 (mm3/min) scores 3 marks
or
13.2 (mm/min)
ignore or 12.6
1
13.2 × 0.8
allow their calculated value in the range from 12 to 14 × 0.8
1
10.56 (mm3/min)
allow 10.6 or 11
1
(f) no photosynthesis
allow plants need light for photosynthesis
1
Page 51 of 64
(so) stomata closed (as no carbon dioxide needed)
1
(so) no transpiration
allow very little transpiration or little water lost
1
[13]
Q6.
(a)
1
(b) Level 2: Scientifically relevant facts, events or processes are identified and
given in detail to form an accurate account.
3−4
Level 1: Facts, events or processes are identified and simply stated but their
relevance is not clear.
1−2
No relevant content
0
Indicative content
For Level 2 reference to how to focus the slide / cells and achieve
magnification of ×50 is required
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(c) any three from:
Q7.
(a) 6CO2 + 6H2O ⟶ C6H12O6 + 6O2
1
(b) endothermic
1
Page 53 of 64
allow use a measuring cylinder / capillary tube /
(gas) syringe
1
Page 54 of 64
produced as light distance doubles so 2 bubbles
would be expected
(control variables)
max 2 marks for control variables
• temperature of solution
• same plant
allow type / size of plant
Q8.
(a) carbon dioxide + water → (glucose) + oxygen
allow reactants in either order
allow correct formulae, balancing not required
1
(b) chlorophyll
1
Page 55 of 64
B black / blue-black
1
[8]
Q9.
(a) temperature
1
(b)
allow 1 mark for
(c) 2.3(333)
1
or
keep the lamp the same distance from the pondweed each time
1
[8]
Q10.
(a) any one from:
• asthma attack
allow whether asthma medication has been taken
or not
• higher pollution levels
• higher stress level
• developed a cold
• lower air temperature
allow colder day
• time of day
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ignore references to alcohol / caffeine
1
(b) 473 =
1
or
(mean = 473)
473 × 3 = 1419 (1 mark)
or
well ventilated
allow to maintain a steep diffusion gradient
1
Q11.
(a) releases energy (to the surroundings)
allow transfers (thermal) energy to the
surroundings
ignore transfers energy unqualified
1
Page 57 of 64
allow (because) fermentation is an anaerobic
reaction
allow to prevent aerobic respiration
1
Q12.
(a) any two from:
• sex
• age
• fitness levels
Ignore reference to healthier unqualified
allow gender
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allow level of exercise
• weight
allow BMI
ignore height / size
• smoking
allow use of caffeine / drugs
2
Page 59 of 64
allow increase in metabolic rate
1
[9]
Q13.
(a) control
1
to check that the indicator colour does not change on its own
or
to check any changes in colour are due to the organisms
1
(b) (tube) E
1
(c) the amount of carbon dioxide produced by respiration equalled amount absorbed for
photosynthesis
1
[6]
Q14.
(a) (to) stop them falling in the solution
or
0 marks:
No relevant content
Indicative content
• water droplet moves towards the maggots / boiling tube
Explanation:
• the oxygen in the boiling tube is used up in respiration
• (and) the carbon dioxide released from respiration is absorbed by
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solution A
• which causes a pressure difference
• so air is drawn into the tube
• bringing the water droplet with it.
4
(e) 10.5
allow range 10.4–10.8
1
[8]
Q15.
(a) (i) without oxygen
allow not enough oxygen
ignore air
ignore production of CO2
ignore energy
1
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allow removes (more) CO2 / lactic acid / heat as an
alternative for either marking point one or two, once only
Q16.
(a) LHS: carbon dioxide AND water
in either order
accept CO2 and H2O
allow CO2 and H2O
if names given ignore symbols
do not accept CO2 / H2O / Co / CO
ignore balancing
1
or
use of figures, ie
max. production at 40 °C
or maximum rate of 37.5 to 38
1
(ii) 25 – 35 °C
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40 – 50 °C
Q17.
(a) (i) 6 peaks in heart rate
accept 6 increases / spikes or goes very high 6 times
allow heart rate increases each time he runs
1
(ii) 2.5 / 2½
allow 2 minutes 30 seconds
do not accept 2.3 / 2:3 / 2.30
1
(b) more / faster / a lot must be stated at least once for full marks
(more) carbon dioxide / heat / lactic acid removed (from muscles) or more cooling
Q18.
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insufficient / no oxygen available
1
or
respires anaerobically
1
[2]
Q19.
(a) any two from:
• age
• gender
• mass
• number in group
• time
2
(c) (Atkins)
costs least
1
mass loss very similar to other diets or second highest mass loss
or as effective as other diets
1
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