HOSPITAL PAKAR PERDANA - LAB DEPT

DR. MOHD KAMAL ARIFF BIN ABDUL GHANI

KPJ PERDANA SPECIALIST HOSPITAL
Lot PT.37 & PT.600, Seksyen 14, Jalan Bayam,
Kota Bahru, Kelantan

Patient Name : ANUAR HUSSAINI BIN ABDUL LATIF

Ward / Clinic : ACCIDENT & EMERGENCY DEPARTMENT Lab No. : 0720628502
Age : 31Y Sex : Male Date Received : 05/11/2020 14:21
IC No. : 890314-11-5167 MRN : 183079 Date Reported : 06/11/2020 16:41
Report Status : Final

Result Unit Reference range

PCR for 2019-NOVEL CORONAVIRUS, COVID-19/SARS-CoV-2 DETECTION

Sample Type Nasopharyngeal and Oropharyngeal Swab
Sarbecovirus Envelope, E protein gene Not detected
COVID-19 Nucleocapsid, N protein gene Not detected
COVID-19 RNA-dependent RNA Not detected
polymerase, RdRp gene
2019-novel coronavirus, COVID-19 RNA Not detected
Methodology:
By Seegene Allplex 2019-nCoV (COVID-19/ SARS-CoV-2) Assay
This one-step, multiplex real-time, RT-PCR assay simultaneously detect and identify 3 target genes specific for COVID-19, which
include the Sarbecovirus envelope protein gene (E-gene), COVID-19 nucleocapsid protein gene (N-gene) and COVID-19 RNA-
dependent RNA polymerase gene (RdRp-gene).

This novel coronavirus, initially designated as 2019-nCoV that was first detected in Wuhan City, Hubei Province, China at the
end of 2019. The virus has been named as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Coronavirus
Study Group (CSG) of the International Committee on Taxonomy of Viruses (ICTV) and the disease caused has been named
by World Health Organization (WHO) as Coronavirus Disease 2019 (COVID-19). A false negative result may occur if inadequate
viral load is present in the specimen as a result of the following reasons: Improper sample collection, or collection of sample at
an early stage of illness, especially before the symptom onset, or collection of sample at late stage of illness, especially after the
symptom relief, or specimens was not handled and transport appropriately. RNA viruses in particular show substantial genetic
variability. Although efforts were made to design RT-PCR assays to conserved regions of the viral genomes, variability resulting in
mis-matches between the primers and probes and the target sequences can result in diminished assay performance and
possible false negative results. A single negative COVID-19 test (especially if from upper respiratory tract specimen), or a positive
test result for another respiratory pathogen result does not exclude COVID-19 infection.

If there is a strong clinical, and/or epidemiological suspicion of COVID-19 infection, the submission of a new specimen and
request of Covid-19 testing is highly recommended. Expectorated sputum should be collected from patients with productive cough
and the induction of sputum is not recommended. A lower respiratory tract aspirate or bronchoalveolar lavage should be
collected from patient who is intubated. A confirmed case of Covid-19 is defined as a person with a positive Covid-19 RT-PCR.
The symptoms and physical signs, stage or progress of disease, epidemiological links, other laboratory examinations and
therapeutic reactions of the patients should be comprehensively considered during their clinical diagnosis and treatment.
Commercial multiplex PCR assays for respiratory pathogens may detect endemic human coronaviruses (HCoV), known to
cause mild to moderate respiratory tract infections. These are the beta-coronaviruses HCoV-OC43 and HCoV-HKU1, and the
alpha-coronaviruses HCoV-229E and HCoV-NL63. It is important that positive results for these viruses should not be confused
with COVID-19.

References:
1. World Health Organization. Novel Coronavirus (2019-nCoV) technical guidance. https://www.who.int/emergencies/diseases/
novel-coronavirus-2019/technical-guidance (Accessed on February 04, 2020).
2. Corman V, Bleicker T, Brünink S, Drosten C, Landt O, Koopmans M, et al. Diagnostic detection of Wuhan coronavirus 2019 by
real-time RTPCR 2020 [17 January 2020].
Available from: <https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-
v1991527e5122341d99287a1b17c111902.pdf>

Validated by Dr. Muhammad Nazri Aziz (Medical Microbiologist) on 06/11/2020 4:41:17 PM

Printed by Muhammad Haikal bin Yahya on 08/11/20 11:42:41 AM Page 1 of 2

COMPUTER GENERATED REPORT. NO SIGNATURE REQUIRED.

Address:- Lot PT. 37 & Pt. 600, Seksyen 14, Jalan Bayam, 15200 Kota Bharu, Kelantan Tel: 09-7458000 Fax: 09-747 2877
 HOSPITAL PAKAR PERDANA - LAB DEPT

DR. MOHD KAMAL ARIFF BIN ABDUL GHANI

KPJ PERDANA SPECIALIST HOSPITAL
Lot PT.37 & PT.600, Seksyen 14, Jalan Bayam,
Kota Bahru, Kelantan

Patient Name : ANUAR HUSSAINI BIN ABDUL LATIF

Ward / Clinic : ACCIDENT & EMERGENCY DEPARTMENT Lab No. : 0720628502
Age : 31Y Sex : Male Date Received : 05/11/2020 14:21
IC No. : 890314-11-5167 MRN : 183079 Date Reported : 06/11/2020 16:41
Report Status : Final

Result Unit Reference range

Consultant Pathologist:-
Dr. Muhammad Nazri Bin Aziz; MD (USM), MPath. Med. Microbiology (UKM), Consultant Medical Microbiologist

Printed by Muhammad Haikal bin Yahya on 08/11/20 11:42:41 AM Page 2 of 2

COMPUTER GENERATED REPORT. NO SIGNATURE REQUIRED.

Address:- Lot PT. 37 & Pt. 600, Seksyen 14, Jalan Bayam, 15200 Kota Bharu, Kelantan Tel: 09-7458000 Fax: 09-747 2877