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IS 3025 (Part 28) (1986, Reaffirmed 2003): Method of

Sampling and Test (Physical and Chemical) for Water and
Wastewater, Part 28: Sulphites (First Revision). ICS
13.060.50

“!ान $ एक न' भारत का +नम-ण”

Satyanarayan Gangaram Pitroda
“Invent a New India Using Knowledge”

“!ान एक ऐसा खजाना > जो कभी च0राया नहB जा सकता ह”

है”
ह
Bhartṛhari—Nītiśatakam
“Knowledge is such a treasure which cannot be stolen”
 (Fifth Reprint MAY 2008) IS : 3025 (Part 28) - 1986
UDC 628'1/'3: 543'3 [546'224]
(Reaffirmed 2003)

I ndian Standard

METHODS OF SAMPLING AND TEST'

( PHYSICAL AND CHEMICAL) FOR WATER
AND WASTEWATER
PART 28 SULPHITES

(First Revision)
M
e~~--------------------------------------------------------------------------~
10
C\J 1. Scope - Prescribes iodide-iodate titrimetric method for determination of sulphite in water and
g wastewater. This method is applicable to a minimum detectable concentration of 2 mgjl sulphite.
u
u 2. Theory and PrinCiple - Acidified water sample containing sulphite reduces iodate to iodine. Any
~ excess of iodate added after all the sulphite is used up, oxidizes iodide to free iodine under acidic pH
condition. Iodine oxidizes sulphite to sulphate. Any excess iodine results in the formation of blue
~ colour in presence of starch indicator,
"t-

o.. 3. Interferences
10
~ 3.1 I nterferences due to the presence of sulphides may be removed by the addition of zinc ccetate,
o
u 3.2 Copper ions rapidly accelerate the oxidation of sulphite ions. Certain heavy metals may also react
~ in a similar manner as copper. Proper sampling and immediate fixing by acid addition should minimise
~ such difficulties.
!£:
~ 3.3 Addition of sulphamic acid helps to eliminate interference due to nitrate.
t:
(';l

Q; 4. Sampling and Storage

-;0
S: 4.1 Highly polluted or contaminated samples should be stored at low temperature, allowing as little
_2 contact with air as possible.
'in
~ 42 Since at pH 8'0 or above sulphite may be oxidised to sulphate, pH should, therefore be adjusted
'0 below S·O.
(/)
~

~ 4.3 Fix the sample (at about 50°C~. immediately by adding 1 ml ETDA solution for each 100 ml of
(j) sample,
~
...
2 5. Reagents
(l)
t:
(1 5.1 Sulphuric Acid
<$
c-J 5.2 Starch Indicator Solution - Add 5'0 g starch to 800 ml boiling distilled water and stir. Dilute to
g one litre and boil for a few minutes and let settle overnight. Use the clear supernatant liquid. Add a
u few drops of chloroform or salicylic acid ( 1'3 g/I ) to preserve the indicator.
r£
~
5.3 Standard Iodide-Iodate Solution (0'0125 M) - Dissolve 0'4458 9 primary grade potassium iodat~
E (dried for 4 h at 120°C), 4'35 9 of potassium iodide and 310 mg sodium bicarbonate in distilled water
g
u
and dilute to 1 000 mL One mil\ilitre of this solution is equivalent to 500 mg of sulphite,

~ 5.4 Suiphamic Acid - Crystalline.

o

~ 5,5 EOTA Reagent - Dissolve 2'5 g of disodium EDTA in 100 ml of distilled water.
(l)

~ 6. Procedure
5:
6.1 Add 1 ml sulphuric acid and about 0'1 9 of sulphamic acid crystals into a 250-ml long necked flask.

6.2 Measure a suitable volume of ETDA stabilized sample (50 ml to 1CO ml) (see 4.3) and transfer to
the flask keeping the tip of the pipette below the surface of liquid. Add 1 ml of starch indicator
solution (see 5.2).

Adopted 28 November 1986 © October 1987, BIS Gr t

BUREAU OF INDIAN STANDARDS

MANAK SHAVAN. 9 BAHADUR SHAH ZAFAR MARG
NEW DELHI 110002
IS : 3025 ( Part 28) -1986
6.3 Titrate immediately with postassium iodide-iodate standard solution until a faint permanent blue
colour develops (view the colour change against a white background). Carry out a blank, using
distilled water instead of sample.

1. Calculation
50 = (A-B) x ~ x 40000 (mgtl)
3

where
A = volume in ml of standard iodide-iodate solution used for sample,
B = volume in ml of standard iodide-iodate solution used in blank,
N = normality of potassium iodide-iodate solution, and
V == volume in ml of sample taken for the test.

EXPLANATORY NOTE
Sulphites may occur in natural waters or wastewaters as a result of industrial pollution and in
treatment plant effluents dechlorinated with sulphur dioxide. Control of sulphite ion in wastewater
treatment and discharge may be important environmentally, mainly because of its toxicity to- fish and
other aquatic life and its rapid oxygen demand. The presence of other oxidizable materials, such as
sulphide, thiosulphate, Fe H ions can apparently give higher readings of sulphites. Some metal ions
like CU'H may catalyze the oxidation of sulphite.

This method supersedes clause 21 of IS: 3025-1964 'Methods of sampling and test (physical
and chemical) for water used in industry'.

Printed by the Manager, Govt. of India Press, Faridabad