PHARMACEUTICAL BIOTECHNOLOGY

VI SEMESTER
I SESSIONAL QUESTION BANK
UNIT I
10 MARKS
1.What are the immobilized enzymes? Discuss the
various techniques for enzyme immobilization?
2.Discuss in detail the types and working principle of
biosensor with examples.
5 MARKS
1.Branches of biotechnology.
2.Production of amylase.
3.Explain in detail about Protein engineering.
4.Restriction endonucleases.
5.Uses of microbes in industry.
2 MARKS
1.Explain Bacterial Conjugation process.
2.Role of biotechnology in pharmaceutical field.
3.Write the application of biosensor.
4.Write the applications of Genetic engineering.
5.Define Transduction process.

UNIT II
10 MARKS
1.Describe the production of Hepatitis-B vaccine by
rDNA technology.

2.Explain the production of Insulin by genetic

engineering technology.

5 MARKS
1.Write in detail about gene transfer methods.
2.Explain the basic steps involved in rDNA technology.
3.Write short notes on production of Interferon.
4.Explain in detail Restriction endo nuclease and plasmid
cloning vector.
5.Write briefly steps involved in Polymerase chain
reaction (PCR).
2 MARKS
1.What is polymerase chain reaction?
2.Define Toxoid.
3.DNA ligase.
4.Define shuttle vectors.
5.Define Plasmid.