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TRUPATH, An Open-Source Biosensor Platform For Interrogating The GPCR Transducerome - Nature Chemical Biology
TRUPATH, An Open-Source Biosensor Platform For Interrogating The GPCR Transducerome - Nature Chemical Biology
Abstract
https://www.nature.com/articles/s41589-020-0535-8 1/13
09/02/2024, 17:45 TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome | Nature Chemical Biology
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Data availability
All data that were generated or analyzed during this study are included in this Article
and its Supplementary Information files or are available from the corresponding
authors upon reasonable request. All TRUPATH sensors are available to academic and
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non-profit institutions as a kit through Addgene (https://www.addgene.org/).
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Acknowledgements
transfers to third parties. Some third parties are outside of the European Economic Area, with
varying standards of data protection.
We thank M. Bouvier for the gift of the Gβ1 and Gγ2-GFP2 constructs, T. Kenakin for
See our privacy policy for more information on the use of your personal data.
insights regarding quantitative pharmacology and J. Aubé for the gift of ML139. We
for the
also thank A. Inoue for further information
donation and
of the to change your
HEK293ΔG cells.choices.
Due to space constraints
we could not include all citations; for this we apologize.
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09/02/2024, 17:45 TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome | Nature Chemical Biology
Author information
National Institute of Mental Health Psychoactive Drug Screening Program, University of North Carolina Chapel Hill School of
Medicine, Chapel Hill, NC, USA
Samuel T. Slocum & Bryan L. Roth
Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA
John D. McCorvy
Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill,
NC, USA
Bryan L. Roth
Contributions
R.H.J.O., J.F.D., J.G.E., B.L.R. and R.T.S. developed the concept and designed the
experiments. R.H.J.O. and J.F.D. performed the bulk of the molecular biology and
experimentation, with A.M.G., B.E.K., S.T.S., T.C., J.D.M., A.C.G. and R.T.S. contributing
to the in vitro pharmacology assays. J.G.E. established the cloning strategy and
helped with construct generation. R.H.J.O., J.F.D., J.G.E., B.L.R. and R.T.S. wrote and
edited the manuscript. This work was supported by NIH grants (nos. R37DA035764,
U24DK116195 and RO1MH112205 to B.L.R., KO1MH109943 to R.T.S. and F31NS093917
to R.H.J.O.)
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Competing interests
https://www.nature.com/articles/s41589-020-0535-8 10/13
09/02/2024, 17:45 TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome | Nature Chemical Biology
R.H.J.O., J.F.D., J.G.E., B.L.R. and R.T.S. are inventors of the TRUPATH technology and
could receive royalties. These relationships have been disclosed to and are under
management by UNC-Chapel Hill.
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Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims
in published maps and institutional affiliations.
Supplementary information
Supplementary Information
Supplementary Tables 1–8, Figs. 1–24 and Note.
Reporting Summary
Supplementary Dataset 1
TRUPATH transducerome profiling analysis of β2AR, NT1R, LPAR6 and 5-HT7.
Supplementary Dataset 2
Statistical analysis of transducer-specific efficacy (Emax) values reported in
Supplementary Data Set 1.
Supplementary Dataset 3
Statistical analysis of transducer-specific potency (log EC50, M) values reported in
Supplementary
Your privacy,Data Set 1.choice
your
Supplementary
We Dataset
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Comparisons of ligand efficacy (Emax, % maximal transducer response from
By accepting optional
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Table you consent
4.2) between to the processing
transducers, withinofeach
yourdrug.
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Supplementary Dataset 5
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Comparisons of ligand potency (log EC50, M; from Supplementary Table 4.1) between
for further
transducers, within each drug.information and to change your choices.
Supplementary Dataset 6
https://www.nature.com/articles/s41589-020-0535-8 11/13
09/02/2024, 17:45 TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome | Nature Chemical Biology
Issue Date
August 2020
DOI
https://doi.org/10.1038/s41589-020-0535-8
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Katrijn Boon, Nathan optional cookies,
Vanalken ... Tom Van Loy you consent to the processing of your personal data - including
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Allosteric modulation and G-protein selectivity of the Ca2+-sensing
receptor for further information and to change your choices.
https://www.nature.com/articles/s41589-020-0535-8 12/13
09/02/2024, 17:45 TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome | Nature Chemical Biology
Nature (2024)
Nature (2024)
Nature Chemical Biology (Nat Chem Biol) ISSN 1552-4469 (online) ISSN 1552-4450 (print)
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