Table 2.

Calibration of Measuring Pipette

Specification: 10 mL

Table 2.1 Pipetted water volume: 2.0 mL

Trial Mass of Mass of Mass of Volume of Delivered vs Tempe-

No. bottle (mg) bottle + water water water (mL) expected volume rature of
(mg) (mg) of water (mL) water

1 74825.8 76732.3 mg 1906.5 mg 1.91 mL -0.09 30.2 (30.0)

mg

2 74761.0 76691.0 mg 1930.0 mg 1.94 mL -0.06 30.8 (31.0)

mg

3 74815.0 76806.0 mg 1991.0 mg 2.00 mL 0.00 31.0

mg

Table 2.2 Pipetted water volume: 4.0 mL

Trial Mass of Mass of Mass of Volume of Delivered vs Tempe-

No. bottle (mg) bottle + water water water (mL) expected volume rature of
(mg) (mg) of water (mL) wate

1 75281.8 mg 79150.0 mg 3868.2 mg 3.89 mL - 0.11 30.2 (30.0)

2 75333.0 mg 79284.0 mg 3948.0 mg 3.97 mL - 0.03 30.8 (31.0)

3 75328.0 mg 79319.3 mg 3991.3 mg 4.01 mL + 0.01 31.0

Table 2.3 Pipetted water volume: 6.0 mL

Trial Mass of Mass of Mass of Volume of Delivered vs Tempe-

No. bottle (mg) bottle + water water water (mL) expected volume rature of
(mg) (mg) of water (mL) wate

1 80302.5 mg 86191.7 mg 5889.2 5.91 mL - 0.09 mL 30.2 (30.0)

mg

2 80326.5 mg 86219.0 mg 5892.5 mg 5.92 mL - 0.08 mL 30.8 (31.0)

3 80320.0 mg 86223.0 mg 5903.0 mg 5.93 mL - 0.07 mL 31.0

Table 2.4 Pipetted water volume: 8.0 mL

 Trial Mass of Mass of Mass of Volume of Delivered vs Temperature
No. bottle (mg) bottle + water (mg) water (mL) expected volume of water
water (mg) of water (mL)
(Correction)

1 64876.8 mg 72702.3 mg 7825.5 mg 7.86 mL - 0.14mL 30.2 (30.0)

2 64919.3 mg 72716.0 mg 7796.7 mg 7.83 mL - 0.17 mL 30.8 (31.0)

3 65005.0 mg 72819.0 mg 7814.0 mg 7.85 mL - 0.15 mL 31.0

Table 3: Statistical Parameters of water volume (true volume) of 2.0,

4.0, 6.0 and 8.0 mL
Parameters 2.0 mL 4.0 mL 6.0 mL 8.0 mL
True value Average 1.95 mL 3.96 mL 5.92 mL 7.85 mL
(Mean)

Average Correction - 0.05 - 0.04 - 0.08 - 0.15

Standard Deviation 0.046 0.0612 0.010 0.016
Confidence Level (95% 1.95 ± 0.114 3.96 ± 0.152 5.92 ± 0.0248 7.85 ± 0.0397
confidence limit)

Table 4: Linear Regression Analysis for the Calibration of Measuring

Pipet
Slope 0.983
Standard deviation of slope
Y-intercept 0.005
Standard deviation of y-intercept
Regression Equation y=0.983x + 0.005
Correlation Coefficient (r) 0.9999580897
Coefficient of Determination (r²) 0.9999173817

Table. Relationship between X (apparent volume of water) and Y (true volume of water)
for Measuring Pipette
 Sum of values
Volume Intervals

x (apparent volume, mL)

2.0 4.0 6.0 8.0 20

y 1.95 3.96 5.92 7.85 19.68

(true volume, mL)

xy 3.9 15.84 35.52 62.8 118.06

x² 4 16 36 64 120

y² 3.8025 15.6816 35.0464 61.6225 116.153

(y-ŷ)²

Discussion Proper: