NAME: SYED FARHAD HASSAN

CLASS: THIRD YEAR

SECTION: B
SEAT NO: EH2106070

LAB: AMYLASE ACTIVITY

Objective:
The objective of this laboratory experiment is to investigate the enzymatic activity of amylase by
observing its ability to catalyze the hydrolysis of starch.

Theory:

Amylase is an enzyme that plays a crucial role in the digestion of carbohydrates. It catalyzes the
hydrolysis of glycosidic bonds in starch, breaking it down into maltose and other oligosaccharides.
The enzyme is produced in various parts of the digestive system, such as the salivary glands and
the pancreas. The activity of amylase is influenced by factors such as pH and temperature.

Method:
Materials:
• Salivary amylase solution
• Starch solution
• Iodine solution (for detecting the presence of starch)
• Test tubes
• Pipettes
• Water bath
• pH buffer solutions (acidic, neutral, and alkaline)
• Thermometer

Procedure:
• Label the test tubes to indicate the conditions being tested (e.g., pH levels or temperatures).
• In each test tube, mix a specific volume of starch solution with a specific volume of salivary
amylase solution.
• Incubate the test tubes in a water bath set to different temperatures or add different pH
buffer solutions to create varied environments.
• At regular intervals, take samples from each test tube and perform the iodine test to check
for the presence of starch.
Observation:

Starch-Iodine Test:
• Add a few drops of iodine solution to a small sample of the reaction mixture.
• Observe the color change. The presence of starch is indicated by a blue-black color, while
the absence of starch shows a lighter color (yellow to brown).

RESULT: