FARHAINA M.

JULKIPLI BSMT 3

Activity No.5
Isolation Streaking

Procedure:
1. Labeling the plates.
Label each plate close to the edge. Labels should include each of the specimen’s name, date of
inoculation, accession number and the plater’s name or initials.
2. Sterilize the inoculating loop.
Before streaking a specimen, flame the inoculating loops using the alcohol lamp until they are red-hot.
Once sterile, let the loops to cool.
3. Open the culture and collect a sample of the specimen.
A. Broth culture
1. Remove and hold the test tube cap using your right hand’s little finger.
2. Flame the tube mouth using the left hand.
3. Insert the loop into the suspension.
4. Flame again the test tube’s mouth.
5. Replace the cap/ cotton plug, and put the test tube back into the test tube rack.
4. Streak the plate.
During the streaking process, minimize the opening of the lid and the length of time that the agar is
exposed to the open air.
a. Streak the first sector.
Raise the agar plate lid to insert the loop and make the loop touch the first sector of the agar area.
Spread the inoculum in the first sector by moving the loop in a zigzag pattern across the agar plate.
Make the loop movement close together and cover the first region.
b. Sterilize the loop for the second sector.
Remove the loop from the agar plate and sterilize the loop before continuing to the second sector.
Allow the loop to cool off before proceeding to the streaking process.
c. Streak the second sector.
Open the agar plate, insert the loop, and make the loop touch the first sector once, drawing a few
of the inoculum from the first sector into the second sector. The second sector is also streaked
using the zigzag motion but less heavily than in the first sector.
d. Sterilize the loop for the third sector.
e. Streak the third sector.
Open the agar plate and insert the loop. Make the cooled loop touch the second sector once,
drawing a few of the bacteria to the third sector. This last sector has the widest gap, between the
rows of streaking. Once done, sterilize the loop.
f. Final step.
Invert the plate so that the agar is on the up-side and incubate the plates for 18-24 hours.

Observation:
Draw or attached picture of aseptic technique in isolation streaking.

Conclusion:

- Isolation streaking is a microbiological technique used to obtain pure cultures. It helps obtain pure cultures,
enabling the study of individual microorganisms. This technique is essential for accurate identification,
characterization, and further analysis of specific microbial strains. Additionally, isolation streaking allows
researchers to observe the morphology and behavior of isolated colonies, contributing to a better understanding
of microbial diversity and function. In conclusion, it plays a crucial role in studying and identifying
microorganisms by separating individual colonies for further analysis.