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    Enzyme Assay For Enzyme Activity

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    terah debuyan
    This document summarizes several common enzyme assays used to measure enzyme activity, listing the enzyme, assay method, and in some cases the forward and reverse reactions. Assays described include those for creatine kinase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, GGT, amylase, lipase, and glucose-6-phosphate dehydrogenase. A variety of colorimetric, titrimetric, and other methods are provided.

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    Enzyme Assay For Enzyme Activity

    Uploaded by

    terah debuyan
    9 views2 pages
    This document summarizes several common enzyme assays used to measure enzyme activity, listing the enzyme, assay method, and in some cases the forward and reverse reactions. Assays described include those for creatine kinase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, GGT, amylase, lipase, and glucose-6-phosphate dehydrogenase. A variety of colorimetric, titrimetric, and other methods are provided.

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    Enzyme assay for enzyme activity

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    This document summarizes several common enzyme assays used to measure enzyme activity, listing the enzyme, assay method, and in some cases the forward and reverse reactions. Assays described include those for creatine kinase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, GGT, amylase, lipase, and glucose-6-phosphate dehydrogenase. A variety of colorimetric, titrimetric, and other methods are provided.

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    © All Rights Reserved
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    9 views2 pages

    Enzyme Assay For Enzyme Activity

    Uploaded by

    terah debuyan
    This document summarizes several common enzyme assays used to measure enzyme activity, listing the enzyme, assay method, and in some cases the forward and reverse reactions. Assays described include those for creatine kinase, lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, GGT, amylase, lipase, and glucose-6-phosphate dehydrogenase. A variety of colorimetric, titrimetric, and other methods are provided.

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    Enzyme assay for enzyme activity

    Creatinine Kinase
    Tanzer Galvary assay Forward reaction
    Oliver Rosalki (common) Reverse reaction

    Lactate Dehydrogenase
    Wacker method Forward reaction
    Wroblweski Ladue Reverse reaction

    Aspartate Aminotransferase
    Karmen method Malase dehydrogenase

    Alanine aminotransferase
    Reitman-Frankel method DNPH BLUE
    Babson method Diazomuim salt violet
    Karmen-walker method

    Alkaline phosphatase
    Bessy Lowry Breack
    King-Armstrong
    Bowers and McComb Reference method
    Bodansky

    Acid phosphatase
    Nudson P-nitrophenos
    Roy Thymolphthalein monophosphates
    Gutman, King, Armstrong
    Bodansky

    GGT
    Szazs assay y-glutamyl-p-nitroanilide

    Amylase
    Amyloclastic technique
    Saccharogenic method
    Chromogenic method
    Continuous monitoring technique
    Lipase
    Cherry-Crandall method Titrimetric; Measure liberated fatty acid
    Turbidimetric mehods

    Glucose-6-phosphate dehydrogenase
    Red cell hemolysate

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