Aa MODERN SCIENTIFIC EOE Te mee ulus Gaadll s (UURY) Gob LadeY| Jullas ales, dacs Cals jie g Aylarall Sgn de wld Cy 9 ge AS ys CANS) Jullas ales @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 -01021239067 @A MODERN SCIENTIFIC Yow sccute reat our ison brea yyy) errr ry oly ginal 8) gl) Aces Agel sh J Js) epg 3) a) 20 ABAD: gull gay se Scharrer 4 pally LIVI juts st on a ons gi) a (GREEN COFFE — call Lei yo Che) yu 6185) bth vlad Soh G8 gi fay Wats son . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021 ygA MODERN SCIENTIFIC Yow sccute reat our ison AUS Ga ll JUS aa Kjeldahl 4 ss plaka oy sl % Ana sti Bs ee 100 co $Y Leptall ut gLepinnd lial cues fal sins sat] Kjeldahl Mae plas Haak! 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US suction cap (Ss! any jal) Bs toy - Suction cap «tse peel fan y GS iy 9S say 48fs 60 lla peng CBs lla! 20-10 at 2 pb Jalal! pb Anning « ts 4d SuCtiON Cap eli a+ Cxdall dsl Jals Go Gayl! ale ai a8 HN go blidll Gly suction cap WL i Gad has gy Lie igo sete US cal} sll chal) Ge Le 70 lism stay need 9 CU SG ia Hn aye peng Say le Gell ala « pig a5 1) 10 hkl eb Sa yp GR ae ole og sas gl all ss BS! 1 ashi 85.31 g st) 158! 41 493 Sodium hydroxide J-70 aa) 2 70 os sll poe le 3 EAL Ge Mol HBOS Isles de Je 30 cle gs I S00 Te ebay he Sadao am 4 THBBOS Jp a pantie GEN ag GS Gem! 5 B85 5 Sul (NH3 I NHs dese ag ogsGE JS Ga) hil Ia 6 Se (PH 4.4-5.8) 8) ps) petal Go Ua a aha aa gs NHB Uelits Laie 7 cagehal Seg oD aaa oe aba 2 jl gaan Jalna SCIEN Go Je 150 Ne BED 8 AGUS ty pM Byitly ol Sy Baal) La sy § Ls gl 1 wai 355 8403) 2 SY Sse 0.1 HCH plas (g2 yah lll pl) Jalal ong Gf Ja) anil gl Lh ot! HyBOs de 3 Bosna S deal / aia gh pall Yes Ge UB cle Je 0,05 th Mah oS pose Gane Ge Sle dow 4 BaD Aad ss he A ny il CH Jp se a Ly hal By gl aes LS yon Oe Aad o ’ @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021239067MODERN SCIENTIFIC Yow sccute reat our ison Yo Osa Rpt (VS - Vb) x 0.1 x 14.01 wx10 = cae 8 ha Fx % cams Aas = % Bs wl Ad Aol gd dl Ja AS vs 20.2 = ADs yen gd liga) Jal 4.5 VB pall 38s ell Ian 4 pe M cpssily Slop 14.01 Aa te Rea ol om col ol pall Jagat alee 10 Agld Las (96.25 = Gaps gl esl Upp ae F Salas w pa sats Bh pula lb yS a> 0.8 + (K2SO4) Clie » uly a> 7 (Hydrated copper sulfate) catalyst (81/2 380) 9633 55s Sa pee NaOH Methanol J- 75 / Methyl red o- ¢> 0.1 =i! Methyl red Methanol Js 75 / Bromocresol green 0.1.3) "Bromocresol green Bromocresol J- 10 isl pba jbid! Ul, Boric acid gas 40 ed Borie acid “phil ol 1000 gl gelSis Methyl red J: 7 + green aysiu O.1 ge Uslne Hel ow AOAC Official Method 2001.11 Protein (Crude) in Animal Feed, 11 Forage (Plant Tissue), Grain, and Oilseeds. . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021239067A MODERN SCIENTIFIC Yow sccute reat our ison brea yyy) Hal pay Jol ian ela ged Aida Aya th a va a Hah % tea SY gh de desiccator gi lily cela 3 Saal Sy sien 9 105 sie Gil gf only & i) Gull in Edad alll 5 Gi gle Hp jt GLI YD Qe lle ad | Aa ghee ABS sie he Ls 2 aad Gans yl ga eld a Sell gaia capable Sig hig ony Algal) ate y Gobel 3 204 ouawne cial (W1-Wa), 100« = (ly des Gada) Ja taal (a) 059 wi Bisa aes Ail s) Gis w2 Aaa) Cl St) sed at Jee OVEN en A..A.C. Official Methods of Analysis of of Official Analytical Chemists the Association. ’ @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021 ygA MODERN SCIENTIFIC Yow sccute reat our ison ev veel PSY) ern eT Ener ee Fag pall Ay gh gl) 58 waa Ree N tea percerny = 2 sel) AED jansne ll Hall JAS Dine gi onloff 35 <8 Jee) sind tare/stop 7) le bial Ldall soph ie ny! 20) Gadi le Laka 9 inal i 8 nly Galil! Gull aay hy Andie Apel Iam Bel yo Qo hes ial oan oy 5 Gehl! gb Ue im pe 5 it 23 gl ge aise ose UI anll jleall lazy heal gly cod od GHD IS 5120 Gd Let Salles a a3) ely JS) eal) jay Gao a Gil ayy oh aapate diel @ oA Gl Sa yo al GAL 5 heal Sh EEDA) yall ae power 5 de BomNouswns 3 heal Stead aly gl Lead 5 Aaah cy sand oat Ses Kerm(44- MA -15) 4s) as Kern (11- MA -16) 4:5) ies Kern (11-MA -27) isl) Jes es KERN Operation manual . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021 ygMODERN SCIENTIFIC Yow sccute reat our ison A ev veel PSY) EOE TRE n maT ee dla) GUS) waa sh N% te aa ERY) i gh chee yp pl ily ale Sl nadie 2 6001 ade se oa gh Hyd ae 1 Ay (433: 30) desiccator 4 pall a8 Apple 5) dQ) ela Sel 5y Mish Be 15 dss eUasl Agi 62 Y 6 GB pl) ol Aye dg 0 GOD le 6) all Laas Binal B58 ESAT ae a Qh A phe og A ce OA gl eUaaD gai gl sel AES GS 11) gS GM Algal ya Linke Lda y XM Le G13 sl On el wor o ea (W1-W2) x ay 100 Wa (as All Ge Ysa Ait yl Jy wi sl gle Lal ag hy w2 swabs w3 Assia Cy sea se Jan Muffle furnace en Determination of Ash in Animal Feed: AOAC Official Method 942.05 ash of animal feed ’ @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021 ygeh MODERN SCIENTIFIC ‘seal! Scharrer 4iy phl tidy LY) ya Lid) 29 Du gl Lg aly ly eacan I Y(t pba « 5h cal) ga GDN Sl oe ee I i i eS patel Sal ae al ayy pe AN eS oi a A Ga pls p18 LS GLIYN cya 8 V5 gpa AB gall GY AED al gall ann Gif Came gel Aaisa CF Se GAT, ALLY gales ye ut alll gu gh 4S sll CL ll Cae AS Sl ABI) Chabal ao 5988 yal ge aged) GUY! Gin Agyill Ase iabes Yl y Cy lil a Mii Sle gla lal aay 6 petal AYE gt lly aly GUY Ga 6 RUAN Giang hi Gia 9 AN ae Oe gy jy Kind CMe Scharrer GAs lastly Gu! aps pends Hy sl) oe YN ogy Gale ee My le Ob. Aas Re 600 se fo gh lel 3 any ALS Gt waa SUL % Spal ss RSW) cilghs, ones Arad Prony Pd een Pore ach ASHING & Le r | Gk sel Yl gh Aegall 2 gLaSD aLsl Ag au aly He 1 Aapeall ape) Sindae plas peo Pedy (1) Rage ak ie El 3 a dual 1 2 A 3 sis 90 sa ly 48 Sy 2 Scharrer GS + Je 60La) 4 peal yeahs 5 sis ell gs Je 400 ts Ly Lath 6 dpa tl hee JULY Ge 87 eS yee 25 Oye sei Ge a 25S. CDE Lue 8B 2 90 Saud ys Aa ye 130 g Dal A id aaa) O39) (3) a) gb sad Aad oD diy wv o3al sh ’ @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021239067A MODERN SCIENTIFIC Yow sccute reat our ison eal (W2—W3) 100x = ——————— = (4) yi ~ (= 1) Sel 55, Wt HS 90 Salo ta 130 se A A Gin ss LN GL Sia 3p WD Bel D8, ah 2 550 Se ey LM Oy | WS Aeshna Cyd sp aS dy gS Las 1 22500 i pall elally SN 7296 ANS! taam ge Le 365 ASE penal! Ua joule Jy - laa Iie Gu de 450 dani 3028p) oh fll Gan = P12 poh did yy )5 got uae - 545 oS ules 2 ose 3 Ade 4 daa Jee Muffle furnace Magnetic Stirer Velp ew ISO 6541:1981 Agricultural food products - Determination of crude fibre content - Modified Scharrer method VELP Crude Fiber Determination in Feed according to the modified Scharrer method ’ @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021239067A MODERN SCIENTIFIC Yow sccute reat our ison Non Direct Method call U3) aa one % nat GAY) gs a GRY etd yd oe 05a A Meas Saal gd acing oases Saad Uys dae 120 ae Gill ob eo B59 Hall ae 2 Soxhlet jo} WB LY JS 3 LORAIN aay lf 98S ce 500! 5 yy2 SAD i L250 say pls ill AYN Opal Say Uae of ob pany il clad! ity ods Soxhlet eo Uma si ad 5S (GS 15 3b 150 Jana) tol 14 gi a GRAS BE asl ABS oo ay ll 8) 7 ASS Sill Gas LSE a te Fy sh e 2 90-B0 sie Cel Gin 8 al sisal) lial Jal 9 iD Uh es a lI Soy ay 2110 heal (w2-w1) x —=Ha vl) 100 Ww Oo— ANIM Dayal ye doy WI AST 3% gS hin a al le sine ys} W2 Seloiy WB Aakash sad a a Soxhlet apparatus aw Determination of Ash in Animal Feed: AOAC Official Method 942.06 ash of animal feed . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021 [ygA MODERN SCIENTIFIC Yow sccute reat our ison Seale 1 ake jeall ce ppb 0 Lip iil lll ge 2 Se SG 2 alall us (anal 80:20) ele Salas Spied yaad 3 elu B US» plas! AflaTest Jslasslac} 4 wofaal! jhgall ale ppb 0 [Lis (xis ,4 Developer J. 1 + methanol Js 1) & iil! GIS) of yo 855 ADM ele yagi ets (NACI) chee 5 eo Babe Ge oe 505 6 (20:80) ols Sin Ue 100 iting 7 foal Migs tad Abe de yy LIS), LOG Ais SB i le Ae ens Bae ald at) 9 ass gh Se 100 pal 3 inal Galil yo de 1038.10 gp By AE cL Ge Je 40 Hal ala i817 wal (lsh glass syringe 35 phe ji) Gi cle y 8 hy 011.5 ety I aD ot) 12 ke 10 ial Hashes 3 dual AflaTest oS J25 5 Lusky (Auall ap 1.0 = Le 10) gin) ddd Galen) oe 10 py ny od 13 SN IS Ge elaall sis 8 / SLB 21 Joey Ab Jc gh 2-4 ney ASU DE AD Ll de 10 oh 14 AAS se hg oop co SN 5p LAT SLN ) S15 glass syringe HPLC Ji) Je 1.0 Gals aS cate aS! a 16 SN A (Co 1.0) Hip IS ep 9 488 Sk Jae AS) ye Syd 9 17 Si SD Ai tL. AflaTest Developer oJ 1.0 4! 18 Beall A GAS pags lee BEN 19 _AflaTest Developer Sika) 3. 438 60 ou ons DUN 38 5 1.5),20 Aaah Cyd AflaTest Developer .1 HPL Usta 2 Afiatest ls 3 mBetidy 4 oat Jes FLUOROMETER VICAM aus Hansen T.J., Journal of Food Protection, Affinity column cleanup and direct fluorescence -measurement of Aflatoxin M1 in raw milk, 53 (1) (1990) 75-77 Truckess, M. W., Stack. M. E., Nesheim, S., Page, S. W., Albert, R.H., Hansen, T. J. « and Donahue, K. F., Journal of the Association of the Official Analytical Chemistry, Immunoaffinity column coupled with solution fluorometry or liquid chromatography post . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net CSTs a eyA MODERN SCIENTIFIC Yow sccute reat our ison column derivatization for determination of aflatoxins in corn, peanuts and peanut butter: collaborative study, 74 (1) (1991) 81-88 (GREEN COFFE — CAA dai ys cilise) cpus gi LS 9) USS! aa Ppb OHS $1831 pid Badal le (ASM pet — pnt) Ss all (pad ppl plas gules scl) Bast 6 play jp Gl die dee Ata) us J at dS Tween 20 PBS (G1112) 0.1 5 PBS asi 2 plat) cue lg seal JS (spa 80:20) eli Jue HPLC Joist aad 3 ppb O | i (OchraTest Akal J sla. Je 1.5) £ All atts oS ga ss 4 satel) Seal! Se ppb 0.1 Ail lll ow BZ SG 5 ADAM cle yt ees (NaCl) ch ae 5 oD ake die 5008 6 Ages 20:80) ee J 8% Je 100 eal 7 sal Mf De He yay GLI, LOA is 8 Bice i trllens IB EASE A vain ot) 9 ss gl Bi leg gi hall palit nL 10 36.10. dhe BIN PBS od. 40 e881 gl psi Glass syringe is CRM Ay Se 11.5 ot yn EG GD ot) 12 we 10 Gal Basins dual! Jaen OchraTest lS SAA ye Us (jel LAS op 1.0 = de 10) GR Gala oo 10 2 oe S13 ASD se else) op SLE 21 ys ABS / Hib 2-4 Jace aySl JK ge 7 0.1 Aut Tween 20 PBS Jala. oJ 10 en 8.14 AD ge hg pp A ABE / od 1 Jae (hI ID LL LB 15, AS pe elgg 59 i) Hh 2-1 ony SI IS NL og 8 LS 9 16 glass syringe 4 OchraTest shell Ujlas Gods 1.5 Lal OchraTest S395) 3S 17 saeslaj CAS 4 (Le 1,5) Sl Glad US cog 8/5 8 T Joey OchraTest 1S D8 oe yay pla! Head i yl ce ad Sl ayy In bs 18 AasSSuall Cp Tween PBS 1 Tween .2 OchraTest Js 3 at es FLUOROMETER VICAM el * Hansen T.J., Joumal of Food Protection, Affinity column cleanup and direct fluorescence measurement of Aflatoxin M1 in raw milk, 53 (1) (1990) 75-77. . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net CSTs a eyA MODERN SCIENTIFIC Yow sccute reat our ison + Truckess, M. W., Stack. M. E., Nesheim, S., Page, S. W., Abert, R. H., Hansen, T. J. and Donahue, K. F., Journal of the Association of the Official Analytical Chemistry, Immuncaffinity column coupled with solution fluorometry or liquid chromatography post column derivatization for determination of aflatoxins in corn, peanuts and peanut butter: collaborative study, 74 (1) (1991) 81-88. . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net aa eee PloyMODERN SCIENTIFIC Yow sccute reat our ison Lapua Soh as (ob ja yl) BU a UA joel ay BIL JGR) gs coh Ally (63) all dg Git) 50) Let apadaal ell i tS Oe gle O.2 Ge da aie Clie 39 A (B 5A) MSY! yl £30 Se is 30 Had ph an gh eee YS Halt LED I Us LTO GIA atl 2 Agen 2 0.5 GASB ey le clin ey AB He A Cd Je 10 Bel ay 3 (Aids 30 251.6) HES Ssh Sey 4 hese ON oh Le 10 ha Bape gd eal SD Dally A pl GAA sel DL SS Ba 5 GA AY se Bash pasta Aaah 5 8 gs 3 oy ipl dom as B sly A ie S cw Gal) Ge Aa PH Slee S jel SL oy ee 6 lish Joell Degree of baking PH units pH tube A. pHtube B Colour Raw 4.90 8.80 6.90 Red Underbaked 0.80 7.60 6.80 Pink Properly baked 0.30 7.40 6.80 Pink Properly baked 0.08 6.98 6.90 Pale pink Overbaked 0.03 693 690 Amber on) (cli a) Se + Bie) Bl PH - (ull Jslae + de) A asl PH = AU eaten |} + aime] ‘Simin after A ‘Water-bath 30 + §°C 30 min. ‘Sasee |} ssc ‘Moasure pH 0-3 min, ‘Tube A: decant supematant ‘of supernatant liquid [quid into beaker ree —| ‘Calculaa ndex of uraso ntity . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021239067A MODERN SCIENTIFIC Yow sccute reat our ison Aroha dass 190,05 CbusAll Je yloa 41 Np85e glke she ole de 100 Gi gir. (KH2PO4) padi ll Claes gslal pa 3.403 - gis ls Je 100 gi g.t. (K2HPOM) op pall pele liad Oop 4.355 UH - HOA Hasty yas! Stl 5 ye De 10 ele cade oe gel = “shi el de 1000 Gd Steals dy — PSY USS ae Bell 7.0 Miguel GMI eusl — sy 90 atl YI ope alg AQT in thus Jie dslaa 2 AA lal) ca i) J la Le 500 4g. eal Ge ola 15 YH ad adel aig sail Ge 7.0 gies ull ue - 1 ae pat) gi tye 3 eect! oS sya Ula 0,02 Ne 15 ob nol still on ae 0.1 Se = 100 sively ss - set es Water-bath with agitator, #0.5°C accuracy 1 PH measure unit with glass electrode 2 Temperature compensation 3 aa Caskey-Knapp method (1944) modified by AACC (1969) Leticia Comar (Nutrimentos De! Sureste, Merida, Yucatan, Mexico) egal Las) waa) “as gla Sp asa il ie el 1 a 430) 2 0.1 AN gi Ose des yal oe 10 es BS SI AEE ae wk gus vod oek 4 Masia!) alent 1.18 32S; %37 Ul) sa Gas 80.1 Bi gi Isaeslass (BIGM yi ds 100 990.1) au * AOAC. Official Methods of Analysis of of Official Analytical Chemists the Association. « USA.-A.O.CS. Official and Tentative Methods of the American OIL Chemists Soc. USA . @ Office No.306 - Build No.A - Floor 3 - Ali Eldin Mall - 6 October - Giza - Egypt © info@modernscientific.net © www.modernscientific.net 01021600212 - 01021239067