MAGNESIUM CALCIUM PHOSPHORUS

Fiske&Subbarow Method, 5 Minute Test

METHOD Photometric test using xylidyl blue Photometric test using arsenazo III Micro Method, Colorimetric,
binochromatic
Magnesium ions form a purple colored complex with Calcium with arsenazo III at neutral pH yields a blue
xylidyl blue in alkaline solution. In presence of gedta, colored complex whose intensity is directly
PRINCIPLE which complex calcium ions, the reaction is specific. proportional to the calcium concentration.
The intensity of the purple colored complex is directly Interference by magnesium is removed by addition of
proportional to the magnesium concentration. 8-hydroxyquinoline-5-sulfonic acid.
ETHANOLAMINE—pH 11 = 750 mmol/L PHOSPHATE BUFFER-ph 7.5 = 50 mmol/L Phosphorus Reducing Reagent
REAGENTS GEDTA-60 umol/L 8-hydroxyquinoline-5-sulfonic acid = 5 mmol/L Phosphorus Color Reagent
XYLIDYL BLUE-110 umol/L Arsenazo III = 120 umol/L Phosphorus Standard
CHROMOGEN XYLIDYL BLUE ARSENAZO III PHOSPHOMOLYBDIC ACID

Serum, heparin plasma, or urine (NO EDTA

SPECIMEN Serum,plasma, csf, urine (no edta plasma) Serum
PLASMA)
520 nm, 500-550 nm (increase of absorbance)
WAVELENGTH 650 nm, 630-670 nm 600 nm
628 nm, 575-650 nm (decrease of absorbance)

OPTICAL PATH 1 cm 1 cm

TEMPERATURE 20-25C/37C 20-25C/37C

MEASUREMENT Against reagent blank Against reagent blank Against reagent/water blank

CONVERSION 0.2495 (mg/d- mmol/L)

0.4114 (mg/dl-mmol/L) 0.324 (mg/dL-mmol/L)
FACTOR 0.025 (mg/24h-mmol/24h
INTERFERING
Ascorbic acid, bilirubin, lipemia, magnesium,
SUBSTANCES Ascorbic acid, bilirubin, lipemia, calcium, hemoglobin
hemoglobin
SERUM: SERUM/PLASMA:
NEONATES: 1.2-2.6 mg/dl 8.6-10.3 mg/dL SERUM:
REFERENCE
CHILDREN: 1.5-2.3 mg/dl URINE: Adult: 2.5-4.5 mg/dL
RANGE
WOMEN: 1.8-2.6 mg/dl Women: <250 mg/24h Infant and Children: 3.5-6.5 mg/dL
MEN: 1.8-2.6 mg/dl Men: <300 mg/24h
HYPOMAGNESEMIA:
HYPOCALCEMIA:
neuromuscular irritability (tremor, seizures)
osteoporosis
cardia symptoms (tachycardia, arrythmia) LOW:
dialysis patients
CLINICAL HYPERMAGNESEMIA: rickets
defective intestinal absorption
SIGNIFICANCE dehydration
hypoparathyroidism
renal disorders HIGH:
HYPERCALCEMIA:
excessive intake of antacids Severe nephritis
hyperparathyroidism
ASSOCIATED WITH:
malignant diseases with metastases and sarcoidosis
Weakness of reflexes and low blood pressure
Strontium salts in medicine may lead to strongly
NOTES:
increased calcium levels.