BIOSAFETY CABINET

AND
PERSONAL PROTECTIVE EQUIPMENT
 MEMBERS

Jan Leigh Luis Matthew Kaye Anne Karylle Casandra Gabrielle Antonio
Ljorman Musni
Calantuan Cagadoc Bayan Marquez Cruz
Biological hazards, or biohazards, are substances that
pose a threat to human health, including medical
waste, microorganism samples, viruses, or toxins from
biological sources. Biological hazards can come from a
variety of contaminants and pathogens. In order for us
to gain knowledge and study about these dangerous
organisms, several equipment to maintain biosafety
were made.

Biosafety cabinets (BSCs) are one type of

biocontainment equipment used in biological
laboratories to provide personnel, environmental, and
product protection. Most BSCs use high efficiency
particulate air (HEPA) filters in both the exhaust and
supply system to prevent exposure to biohazards.
 Scenario Regarding Biosafety Cabinets
Handling hazardous chemicals.

Let's say you're working on a project

involving the synthesis of a new drug
compound. Unfortunately, one
of the intermediate compounds used
in the reaction process is highly toxic
and poses a significant risk to lab
personnel if not handled properly.

Instruments Equipment Protection and security

 In this case, using a biosafety cabinet
provides an additional layer of protection
against exposure to these hazardous
chemicals. By containing the substances
within the cabinet, you reduce the
chances of accidents occurring and keep
yourself and others safer.
Biosafety cabinets (BSCs)
 CLASSES

Biosafety Class I BSC

cabinets
(BSCs) Class II BSC
Type A1, A2, B1, B2
Biosafety cabinets (BSCs)
are classified into three
primary types: Class I,
Class II, and Class III.
Class III BSC
Class I BSC
Class II biosafety cabinets are most common and widely used in biological research laboratories and are
differentiated into types such as A1, A2, B1, or B2.

CLASS II TYPE A1

intended for routine microbiological work without the

generation of chemical vapors. Can be room re-
circulated or air gap type
 CLASS II TYPE A2

the most common type of Biosafety Cabinet. A portion of the inflow air is
exhausted while another portion is recirculated within the cabinet. Approximately
30% of its airflow within the cabinet. This cabinet exhausts to the outside through
a duct system.
 CLASS II TYPE B1

B1 BSCs have HEPA-filtered downflow air composed mostly of uncontaminated

recirculated inflow air and exhaust most of the contaminated downflow air
through a dedicated duct after passing through a HEPA filter.
 CLASS II TYPE B2

Type B2 cabinet does not recirculate any air within the cabinet. All exhaust
leaves the cabinet through a duct to the outdoors. B2 requires a dedicated
exhaust system and a dedicated remote blower for each cabinet. They have
HEPA-filtered downflow air drawn from the laboratory (not recirculated from the
cabinet exhaust) and exhaust all inflow and downflow air out to the atmosphere
after filtration through a HEPA filter.
Class III BSC
History and Use of 1980s The first cabinets were
introduced in major
Biosafety Cabinets in universities, primarily for
vaccine development and
the Philippines microbiology research.

1990s
The government promoted
their adoption through
training programs and
regulatory frameworks.

Biosafety cabinets are utilized

in various industries, including
Present pharmaceutical manufacturing,
agriculture, and environmental
science.
 Biosafety cabinets are essential laboratory equipment that
protect personnel and samples from biological risks.

Theoretical and Traditional cabinets face issues like limited chemical hazards,
high energy consumption, noise, and reduced airflow.

Technological
Modern designs include ergonomic design, enhanced
Advancements airflow, innovative ventilation systems, and HEPA or high
efficiency particulate air filters.

Regarding Advancements in biosafety cabinet operation include automatic sash

Safety Cabinets positioning, user-friendly controls, and remote monitoring.

Technological advancements in AI, machine learning, sensors, IoT,
self-cleaning, automated cabinets, and virtual reality are enhancing
performance, efficiency, and remote operation.
 Procedures Using Wear protective gear and
follow standard operating
Biosafety Cabinets procedures.

Turn off the UV sterilizer and

The use of a biosafety cabinet can activate the lights on the
lead to contamination and exposure cabinets and blowers.
of personnel to pathogenic
organisms. Proper use of the cabinet
ensures a contamination-free zone
Clean interior surfaces with
by combining air from a HEPA filter
disinfectants.
with recirculating air stream. Before
starting work,

Clean UV light bulbs, disinfect

equipment, and use a UV meter
or monitoring strip to check light
efficiency.
 Criteria for Selecting Biosafety Cabinet

Type and quantity of Accessible components and straightforward

Containment Level maintenance procedures
materials being worked with
 Specification of each class

Class I Biosafety Class II Biosafety Class III Biosafety

Cabinet Cabinet Cabinet

Provides protection to Provides protection to Highest Level of

the environment and the user, environment, Containment.
laboratory personnel. and sample. Suitable for work with
hazardous agents requiring
Biosafety Level 3 or 4.
Guidelines for specific experiments
Biosafety 1 Always wear a lab coat and gloves

Before and after each use,

Cabinet I 2
decontaminate the interior of the
BSC using an approved
disinfectant.

3 Always open the sash to the

correct working height. Adjust it
as needed.

4 Use proper aseptic technique to

prevent contamination
Guidelines for specific experiments
Biosafety Cabinet II
1. Put on PPE accordi ng to your laboratory SOP.
2. Turn UV light OFF (if used).
3. Turn fluorescent light ON.
4. Turn the cabinet ON, allow it to run for 4 minutes to purge the BSC
of particulates.
5. Verify proper sash height and that sash alarm is ON.
6. Adjust stool height and position
7. Disinfect the interior walls and work surface of the biosafety
cabinet with an approved disinfectant.
 Guidelines for specific experiments
Biosafety Cabinet III
1. Conduct a thorough risk assessment
2. Consider the hazardous characteristics of the agent, laboratory procedures, work
practices, safety equipment, and facility safeguards.
3. Follow safe laboratory practices and techniques.
4. Ensure proper aseptic techniques and minimize the generation of aerosols.
5. Use personal protective equipment (PPE) such as gloves, lab coats, and eye
protection.
6. BSL-3 cabinets provide both personnel and environmental protection.
7. Ensure the biosafety cabinet is turned on and wait for 5 minutes before use.
8. Adjust the stool height so that your face is above the lower edge of the sash.
9. Disinfect the interior walls and work surface with an approved disinfectant
10. BSL-3 facilities should have controlled access, negative pressure, and restricted
entry. Design the facility to prevent accidental exposure and minimize contamination.
 Proper Cleaning and
Disinfection

UV Light Exposure
Prevention of
Cross
Contamination

Aseptic Technique
 Minimize Movements

Close Containers and

Prevention of Tubes
Cross
Contamination

Regular Maintenance
Methods of Validating Biosafety Cabinets

HEPA Filter Integrity

Airflow Velocity Testing
Testing
Methods of Validating Biosafety Cabinets

KI Discus Test
Particle Count
 Calibration
and
Certification
of Biosafety
Cabinets

Department of Health
National Sanitation
(DOH)
Foundation Bureau of Quarantine
 Actions to do when an accident happens

Stay Calm
Maintain composure and assess
the situation.
Do not panic; focus on safety.
Exit the BSC Safely
Back out of the BSC carefully.
If possible, close the sash (if it
closes).
Glove Removal and Disposal
Remove and discard your gloves
as biohazardous waste.
Dispose of them according to
your laboratory’s standard
operating procedures (SOP).
Switch Off Equipment
Turn off the alarm (if activated).
Switch off the blower motor to
stop airflow within the cabinet.
Alert Others
Advise other workers in the area
about the cabinet failure.
Ensure everyone is aware of the
situation.
Using PPE when working with biosafety cabinets is essential for
protecting worker safety and preventing the spread of infectious
agents. By wearing appropriate gloves, face shields, lab coats,
gowns, masks, eye protection, and hair restraints, you can
minimize exposure risks and ensure a safer work environment.

Donning is the process of wearing personal protective

equipment (PPE) in a laboratory setting, ensuring tight seams
and no gaps to prevent contamination. It reduces the risk of
accidents.

Doffing involves removing PPE without touching inside surfaces

and washing hands thoroughly to prevent cross-contamination
and compromise biosafety cabinet containment.