Biotechnology & Biotechnological Equipment

ISSN: 1310-2818 (Print) 1314-3530 (Online) Journal homepage: https://www.tandfonline.com/loi/tbeq20

Growth Optimization of Plantlets of Paulownia

Tomentosa Moench After In Vitro Regeneration

Z. Garelkova & T. Naydenova

To cite this article: Z. Garelkova & T. Naydenova (1995) Growth Optimization of Plantlets of
Paulownia Tomentosa Moench After In�Vitro Regeneration, Biotechnology & Biotechnological
Equipment, 9:1, 86-88, DOI: 10.1080/13102818.1995.10818829

To link to this article: https://doi.org/10.1080/13102818.1995.10818829

© 1995 Taylor and Francis Group, LLC

Published online: 15 Apr 2014.

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GROWTH OPTIMIZATION OF PLANTLETS OF
PAULOWNIA TOMENTOSA MOENCH AFTER
IN VITRO REGENERATION
Z. Garelkova, T. Naydenova
Forest Research Institute, Sofia, Bulgaria

ABSTRACT
The influence offive combinations of nutritive medium and DMSO on the growth and the
biomass of plantlets of Paulownia tomentosa were investigated. The main purpose was to
optimize the nutritive regime in the pots during the starting phases of its adaptation in vivo.
Best results were achieved when agent Dlv!SO ·in 1q.J o- 4 M was applied alone and in
combination with nutritive solutions. The above growth was vigorous and the under-ground
one was moderately developed.

Introduction This study is a proportion of serial investi-

The adaptation of Paulownia tomentosa gations on optimizing the growth of some
Moench regenerants is the last stage of the in exotic species with respect to their successful
vitro propagation methods, before their es- introduction into our country.
tablishment in the field and their complete Materials and Methods
introduction into this country.
The experiment was carried out with rege-
Recently, together with different rates of
nerated plantlets of P. tomentosa which were
fertilization, some membran agents such as
transferred into a mixture of sand : perlite :
DMSO, manit, and the like widely used in
soil (1 : 1 : 2). They were bred under green-
vitro techniques, have been applied for this
house conditions, with an optimal hy-
purpose.
drothermal regime (2).
Testing various DMSO concentration with
Five combinations of nutritive medium and
respect to their effects on the growth and
development of species such as pea and
uo- 2 M, uo- 4 M, DMSO concentrations,
were investigated. The medium was the one
maze, indicated that rates higher than 1.10-I
ofMurashige-Skoog (1962) widely used in in
M were lethal, yet those about l.I0- 2 M -
vitro methods. It was enriched with hormones.
l.I0- 4 M were optimal (1, 4). It had been
Tested combinations:
established that DMSO influence on the root
1) reference 1 - H2 0
nutrition and plant productivity (5). The ef-
2) uo- 4 M- DMSO
fects of various DMSO concentrations were
3) uo- 2 M- DMSO
tested on Paulownia tomentosa Moench, yet
4) reference 2 nutritive medium
these were interesting with respect to in-
5) 1.10-4 M- DMSO + nutritive medium
creasing the resistance of this Introduced
6) uo- 2 M- DMSO +nutritive medium
species to winter frosts.
The experiment was repeated with rege-
The purpose of the present study was to
nerants in process of adaptation.
test the effects of combinations of DMSO
agents and nutritive media on the produc- Results and Discussion
tivities of adapted regenerants of Paulownia Two groups of plants of considerably diffe-
tomentosa Moench, after in vitro cultivation. rent increment, (i.e. height), were formed.

Bioteclmol. & Bioteclmol. Eq. 9/1995/l 86

 50
40
,_._
8 4
,_.,,"
...~
~3
.·-
,
'
8
~ 20
.,.,
(j
.5 ,,
10 ' :· -- - -- - - - - --- - - 2
.. ',' __ ........ ------------ 3
27.03 27.04 27.05
I - reference - H 20
2- 1.10-4 M- DMSO
3- 1.10· 2 M- DMSO
4- reference- nutritive solutions (n.s.)
5 -- 1.10·4 M- DMSO + n.s.
6- 1.10- 2 M- DMSO + n.s.
The first one was the group of non fertilized
plants. Generally, it was characterized with
lower values of increment, growth, weight,
and chlorophyll amounts. These values were
higher for the second group. So, the average
height of fertilized plants, without any
DMSO agent (4) was 50.0 em while for the
non-fertilized variants its values were bet-
ween 17.0 and 22.0 em in the experiment
end (Fig. 1, Table).
The references (without DMSO) in each of
the two groups remained with lower values
of the above-mentioned features, which was
indicative of the effects of the two factors in this
case (agent and nutritive medium).
The lower DMSO concentrations with the
non-fertilized variants resulted in more in-
tensive above-ground growth (11.4 g of
green wight versus 9.56 g) and its higher
concentrations - in more intensive under-
ground growth. This is indicative about the
uneffective influence of 1.10·2 M DMSO
with no nutritive solutions available. On the
contrary, with uo- 4 M DMSO the above-
ground parts have been formed with a more
efficient utilization of the this agent that has
resulted in the formation of moderately de-
_6
5
,,
1
27.06 10.08 date
Fig. 2. Growth of seedlings on the different variants.
veloped root system.
Similar regularity was found to exist the
fertilized variants as well. With 1.10·4 M
DMSO a more vigorous above-ground
biomass (25.8 g of green weight versus 20.33
g) was formed, compared with the case of
uo- 2 M DMSO. Besides the root system
was more moderately developed in the first
case (20.3 g versus 21.46 g) which again re-
vealed the better effect of this concentration
on the even distribution of biomass.
Literature references about maze, report a
lethal rate of DMSO of 1.10· 1 M (which
inhibits root development), and an optimal
one - 1.10·2 M for both maze and peas (3).
The optimization of the effect of DMSO con-
centration, while diminishing its values
(down to 1.10 M), which has been noticed at
our experiments, confirms these references.
Grading the effects of kay factors it is pos-
sible to conclude that the most important
factor was irrigation (as under its constant
influence the plants growth), next followed
by the combination of DMSO agent with
nutritive solutions, and the third was the
DMSO concentration of 1.104 M that provided
an optimal conditions for plant development.
87 Bioteclmol. & Biotechnol. Eq. 9/199511
 TABLE
Average height Inere- Biomass of !...seedling
Variants (em) begining-end ment Average leaf sizes Chlorophyl (fresh green weight Total
of experiment (em) above- under-
(27.03- 8.08) lenght, width a b a+b ground ground
reference-H2 0 12.4 17.0 4.6 8.6 7.2 1.23 0.73 1.98 1.70 0.16 1.86
1.1 0-4M DMSO 12.9 22.0 9.1 10.5 8.1 1.16 0.75 1.91 11.43 15.68 27.11
1.10-2M DMSO 15.6 21.2 5.6 10.0 8.6 1.44 0.85 2.29 9.56 22.41 21.97
reference- 11.0 50.0 39.0 12.2 9.9 1.45 0.95 2.40 8.18 1.97 10.15
nutritive
solution (n.s.)
1.1 0-4M DMSO 15.4 55.5 41.1 11.8 10.2 1.48 0.99 2.47 25.77 20.35 46.12
+n.s.
1.10-2M DMSO 17.0 59.0 42.0 12.2 10.1 1.46 0.91 2.37 20.33 21.46 41.79
+n.s.

The variant with fertilization and sprin-
kling with 1.10·4 M DMSO provided optimal
conditions for intensive growth (in 1 month)
and high increment of the regenerants.
REFERENCES
1. Atanassov At. (1988) Biotechnologia v rastenievod-
stvoto, Zemizdat, Sofia, 122,216.
2. Gantchev P., Garelkova Z., Gyuleva V. (1989)
Biotechnology and bioindustry, 6, 23-26.
3. Ivanov A.F. (1970) Rost drevesnih rastenii i kislotnost
pochvi, p. 37.
4. Kalinin F., Sarnachkaya V. et al. (1980) In vitro
methods in the plant's physiology, p. 371.
5. Gradinarski L. (1984) In: Proceeding of the Second
International Symposium on Plant Nutrition, vol. 4, Sofia,
395-398, 399-402.

Biotechnol. & Biotechnol. Eq. 9/199511 88