MECHANISM

OF
SEMI-CONSERVATIVE REPLICATION
 DNA HELICASE IN REPLICATION

Bases:

G
Complementary
Base Pairing
C

T
DNA HELICASE IN REPLICATION

Hydrogen bonds broken

 DNA HELICASE IN REPLICATION

Topoisomerase
Role:
It breaks and rejoins
the DNA strands
Function:
It enables tension
caused by
DNA Helicase unwinding to be
Breaking the hydrogen bonds as a result released
breaking apart the double helix structure.
DNA HELICASE IN REPLICATION

Single Strand

Single-strand
binding protein
Role:
It binds to separated
Replication DNA strands to
Fork stabilize them.
Function:
It prevents original
strands reforming
complementary base
pairs.

Single Strand
DNA POLYMERASE IN
REPLICATION
ACTIVATED NUCLEOTIDE
 DNA POLYMERASE IN REPLICATION

Exposed unbound Joining platform for

nucleotides new strands to
assemble themselves

Free activated
nucleotides

Pair up with
available
complementary
base pair on
single strand
DNA POLYMERASE IN REPLICATION

Water

The phosphodiester bond is

created between sugars of one

nucleotide and phosphate group

of other in condensation reaction

as it produces H2O.
 DNA POLYMERASE IN REPLICATION

Joining of adjacent Making new

polymers
nucleotides is catalysed
by DNA Ligase and DNA
polymerase turns it into a
polymer.

The new strand is same

as original because of
complementary base
pairing.
DNA POLYMERASE IN REPLICATION
DNA POLYMERASE IN REPLICATION

Blue strand = old strand

Red strand = new strand

Half conserved

Half new