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Slides PA
Slides PA
Potentials
Jason Ryan, MD, MPH
Cardiac Action Potential
• Changes in membrane voltage of cell
• Transmit electrical signals through heart
• Triggers contraction of myocytes
Myocyte Action Potential
Atrial/ventricular myocytes
Phase 1
IK+ (out) Phase 2
ICa+ (in) & IK+ (out)
0mv
Phase 3
Phase 0
IK+ (out)
INa+ (in)
-85mv
Phase 4
Phase 4
• Resting potential: about −85mV
• Constant outward leak of K+
• “Inward rectifier channels”
• Na+ and Ca2+ channels are closed
Phase 4 Phase 4
Phase 0 Phase 0
Phase 1
Phase 2
• L-type Ca2+ channels open → inward Ca2+ current
• Contraction trigger: excitation-contraction coupling
• K+ leaks out (down concentration gradient)
• Delayed rectifier K+ channels
• Balanced flow in/out = plateau of membrane charge
• Verapamil/Diltiazem = block L-type Ca channels
Phase 2
Phase 3
• Ca2+ channels inactivated
• Persistent outflow of K+
• Resting potential back to −85 mV
• Class III antiarrhythmic drugs: block K channels
Phase 3
Skeletal Muscle
• No plateau (phase 2)
• No gap junctions
• Each cell has its own NMJ
Na+ K+
Refractory Period
• Phase 0 until next possible depolarization
• Determines how fast myocyte can conduct
• Many antiarrhythmic drugs prolong refractory period
Refractory Period
Myocyte Action Potential
Atrial/ventricular myocytes
0mv
Phase 0
Phase 3
ICa
Threshold
IK
-40mv
Phase 4
-85mv If (Na)
Pacemaker Action Potential
SA node, AV node
• Automaticity
• Do not require stimulation to initiate action potential
• Capable of self-initiated depolarization
• No fast Na+ channel activity
• Fewer inward rectifier K+ channels
• Membrane potential never lower than −60 mV
• Fast Na+ channels need −85 mV to function
Pacemaker Action Potential
SA node, AV node
0mv
Phase 0
ICa
Threshold
-40mv
-85mv
Pacemaker Action Potential
SA node, AV node
0mv
Phase 0
ICa
Threshold
-40mv
-85mv
Pacemaker Action Potential
SA node, AV node
0mv
Phase 0
ICa
Threshold
-40mv
-85mv
Beta Blockers
• Modify slope of phase 4
• Less slope → longer to reach threshold → ↓ HR
Beta Blocker
Normal
Slower phase 4
(less slope)
Beta Blockers
• Also prolong repolarization
• Slow AV node conduction
Beta Blocker
Normal
Slower repolarization
(slows conduction)
Slope of Phase 4
Sinus Node
• Changes in slope modify heart rate
• Decrease slope (slower rise)
• Parasympathetic NS, beta blockers, adenosine
• Increased slope (faster rise)
• Sympathetic NS, sympathomimetic drugs
Phase 4
Pacemakers
• Many cardiac cells capable of automaticity
• SA node normally dominates
• Fastest rise in phase 4
• Controls other pacemaker cells
• Pacemakers: SA Node > AV Node > Bundle of HIS
• SA node (60-100 bpm)
• AV node (40-60 bpm)
• HIS (25-40 bpm)