Biotechnology Principles and Processes, Biotechnology and its

Applications
(1) Anode 24. The DNA fragments separated by
(2) Cathode electrophoresis can be visualized by staining with
(3) Don't move (1) haematoxylin
(4) None (2) Geimsa
17. Negative charge of DNA is due to the presence (3) Gram's stain
of (4) Ethidium bromide
(1) Hydroxyl group (2) Phosphate group 25. DNA stained with ethidium bromide can be
(3) Amino group (4) All of these visualized in
18. Given below are two statements : one is labelled (1) Visible light
as Assertion (A) and the other is labelled as Reason (2) UV light
(R) (3) IR light
Assertion (A) : Alpha- lactalbumin was nutritionally (4) X ray
a less balanced product to human baby. 26. The given below figure is the diagrammatic
Reason (R) : First transgenic cow, Rosie, produces representation of the E.Coli vector pBR 322. Which
human protein-enriched milk. one of the given options correctly identifies its
In the light of the above statements, choose the certain component (s):
correct answer from the options given below :
(1) Both (A) and (R) are correct but (R) is not the correct
explanation of (A)
(2) (A) is correct but (R) is not correct
(3) (A) is not correct but (R) is correct
(4) Both (A) and (R) are correct and (R) is the correct
explanation of (A)
19. In gel electrophoresis DNA fragments separate
according to their
(1) Size (1) Hind III, EcoRI - selectable markers
(2) Number of DNA fragments (2) ampR, tetR - antibiotic resistance genes
(3) Only cathode (3) ori-original restriction enzyme
(4) None of these. (4) rop - reduced osmotic pressure
20. Which of the following is the matrix of DNA gel 27. Process by which E. coli take up foreign DNA
electrophoresis (1) Transcription
(1) Starch (2) Translation
(2) Alum (3) Transformation
(3) Agarose (4) Transfer
(4) Cesium chloride 28. Given below are two statements : one is labelled
21. Agarose is extracted from as Assertion (A) and the other is labelled as Reason
(1) E.coli (R)
(2) S.typhimurium Assertion (A) : Very low concentration of a bacteria
(3) Yeast or virus can be detected by samplification of their
(4) Sea weeds nucleic acid.
22. Agarose is chemically a Reason (R) : ELISA based on the principle of
(1) Monomer antigen-antibody interaction.
(2) Dimer In the light of the above statements, choose the
(3) Polymer correct answer from the options given below :
(4) Oligomer (1) Both (A) and (R) are correct but (R) is not the correct
23. In agarose gel electrophoresis the DNA fragment explanation of (A)
which moves farthest from loading place (2) (A) is correct but (R) is not correct
(1) Largest (3) (A) is not correct but (R) is correct
(2) smallest (4) Both (A) and (R) are correct and (R) is the correct
(3) no information for size can be given explanation of (A)
(4) medium size

By – Anmol Sharma Sir 2|P a g e